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Literature summary for 3.8.1.8 extracted from
- Catalytic improvement and structural analysis of atrazine chlorohydrolase by site-saturation mutagenesis (2016), Biosci. Biotechnol. Biochem., 80, 1336-1343 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 (DE3) | Haematococcus lacustris |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D30G/G147D/G162E/A170V/H406P/V466A | specific acitivity of the mutant enzyme is 3.2fold higher than activity of wild-type enzyme. The kcat/Km-value is 3.0fold higher than the wild-type value | Haematococcus lacustris |
| D30G/I393P/L395S/N429S/V466A | specific acitivity of the mutant enzyme is 3.5fold higher than activity of wild-type enzyme. The kcat/Km-value is 3.2 fold higher than the wild-type value | Haematococcus lacustris |
| D30G/Q71G/M315I/R389C/H399Q/N429S/V466A | specific acitivity of the mutant enzyme is 2.9fold higher than activity of wild-type enzyme. The kcat/Km-value is 2.6fold higher than the wild-type value | Haematococcus lacustris |
| D30G/Q71P/R271C/L288Q/M337T/R389C/H399Q/N429S | specific acitivity of the mutant enzyme is 2.5fold higher than activity of wild-type enzyme. The kcat/Km-value is 2.6fold higher than the wild-type value | Haematococcus lacustris |
| D30G/Q91M/T195A/N429S/M99T/R290H | specific acitivity of the mutant enzyme is 1.2fold higher than activity of wild-type enzyme. The kcat/Km-value is 1.4fold higher than the wild-type value | Haematococcus lacustris |
| G55S/I393W/L395R/M315I/E433K | specific acitivity of the mutant enzyme is 4.0fold higher than activity of wild-type enzyme. The kcat/Km-value is 3.3 fold higher than the wild-type value | Haematococcus lacustris |
| Q71P/D128Y/I194V/T195A/M315I/R389C/H399Q/A470T | specific acitivity of the mutant enzyme is 2.3fold higher than activity of wild-type enzyme. The kcat/Km-value is 2.7fold higher than the wild-type value | Haematococcus lacustris |
| Q71R/V58A/R389C/I209M/E317G | specific acitivity of the mutant enzyme is 2.4fold higher than activity of wild-type enzyme. The kcat/Km-value is 2.9fold higher than the wild-type value | Haematococcus lacustris |
| Q7R/V12A/S307P/M337T/I393R/L395R/S438G/R462W | specific acitivity of the mutant enzyme is 3.5fold higher than activity of wild-type enzyme. The kcat/Km-value is 3.3fold higher than the wild-type value | Haematococcus lacustris |
| V12A/Q71A/M315I/M341V | specific acitivity of the mutant enzyme is 5.2fold higher than activity of wild-type enzyme. The kcat/Km-value is 3.9fold higher than the wild-type value | Haematococcus lacustris |
| V12A/Q71T/A173V/M315I/H399Q/N429S/V466A | specific acitivity of the mutant enzyme is 2.5fold higher than activity of wild-type enzyme. The kcat/Km-value is 2.7fold higher than the wild-type value | Haematococcus lacustris |
| V12A/Q71T/M315I/H399Q/N429S/V466A | specific acitivity of the mutant enzyme is 2.4fold higher than activity of wild-type enzyme. The kcat/Km-value is 2.6fold higher than the wild-type value | Haematococcus lacustris |
| V12A/Q71T/T121A/A173V/M315I/H399Q/N429S/V466A | specific acitivity of the mutant enzyme is 2.9fold higher than activity of wild-type enzyme. The kcat/Km-value is 3.0fold higher than the wild-type value | Haematococcus lacustris |
| V12C/A139P/M315I/R389C/I393E/L395R/F439L | specific acitivity of the mutant enzyme is 2.2fold higher than activity of wild-type enzyme. The kcat/Km-value is 2.6fold higher than the wild-type value | Haematococcus lacustris |
| V12F/D30G/V58A/T121A/I393S/L395H | specific acitivity of the mutant enzyme is fold 2.4higher than activity of wild-type enzyme. The kcat/Km-value is 2.7fold higher than the wild-type value | Haematococcus lacustris |
| V12P/D30G/Q71R/H249Y/270V/R389C/I393P/L395P | specific acitivity of the mutant enzyme is 4.2fold higher than activity of wild-type enzyme. The kcat/Km-value is 3.4fold higher than the wild-type value | Haematococcus lacustris |
| V12T/I393G/R389C/L395W/N429S/L446S/V466A | specific acitivity of the mutant enzyme is 3.4fold higher than activity of wild-type enzyme. The kcat/Km-value is 3.8fold higher than the wild-type value | Haematococcus lacustris |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
Atrazine | Q71R/V58A/R389C/I209M/E317G, pH 7.2, 23°C | Haematococcus lacustris |  |
| 0.11 | - |
Atrazine | D30G/Q91M/T195A/N429S/M99T/R290H, pH 7.2, 23°C | Haematococcus lacustris | |
| 0.131 | - |
Atrazine | Q71P/D128Y/I194V/T195A/M315I/R389C/H399Q/A470T, pH 7.2, 23°C | Haematococcus lacustris | |
| 0.132 | - |
Atrazine | V12C/A139P/M315I/R389C/I393E/L395R/F439L, pH 7.2, 23°C | Haematococcus lacustris | |
| 0.138 | - |
Atrazine | D30G/Q71P/R271C/L288Q/M337T/R389C/H399Q/N429S, pH 7.2, 23°C | Haematococcus lacustris | |
| 0.14 | - |
Atrazine | V12T/I393G/R389C/L395W/N429S/L446S/V466A, pH 7.2, 23°C | Haematococcus lacustris | |
| 0.142 | - |
Atrazine | V12A/Q71T/T121A/A173V/M315I/H399Q/N429S/V466A, pH 7.2, 23°C | Haematococcus lacustris | |
| 0.145 | - |
Atrazine | V12A/Q71T/A173V/M315I/H399Q/N429S/V466A, pH 7.2, 23°C | Haematococcus lacustris | |
| 0.148 | - |
Atrazine | D30G/G147D/G162E/A170V/H406P/V466A, pH 7.2, 23°C | Haematococcus lacustris | |
| 0.15 | - |
Atrazine | V12A/Q71T/M315I/H399Q/N429S/V466A, pH 7.2, 23°C | Haematococcus lacustris | |
| 0.15 | - |
Atrazine | wild-type enzyme, pH 7.2, 23°C | Haematococcus lacustris | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| atrazine + H2O | Haematococcus lacustris | - |
hydroxyatrazine + chloride | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haematococcus lacustris | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3 | - |
substrate: atrazine, wild-type enzyme, pH 7.2, 23°C | Haematococcus lacustris |
| 3.6 | - |
substrate: atrazine, D30G/Q91M/T195A/N429S/M99T/R290H, pH 7.2, 23°C | Haematococcus lacustris |
| 6.8 | - |
substrate: atrazine, V12C/A139P/M315I/R389C/I393E/L395R/F439L, pH 7.2, 23°C | Haematococcus lacustris |
| 6.9 | - |
substrate: atrazine, Q71P/D128Y/I194V/T195A/M315I/R389C/H399Q/A470T, pH 7.2, 23°C | Haematococcus lacustris |
| 7.1 | - |
substrate: atrazine, V12A/Q71T/M315I/H399Q/N429S/V466A, pH 7.2, 23°C | Haematococcus lacustris |
| 7.3 | - |
substrate: atrazine, V12F/D30G/V58A/T121A/I393S/L395H, pH 7.2, 23°C | Haematococcus lacustris |
| 7.4 | - |
substrate: atrazine, Q71R/V58A/R389C/I209M/E317G, pH 7.2, 23°C | Haematococcus lacustris |
| 7.6 | - |
substrate: atrazine, D30G/Q71P/R271C/L288Q/M337T/R389C/H399Q/N429S, pH 7.2, 23°C | Haematococcus lacustris |
| 7.6 | - |
substrate: atrazine, V12A/Q71T/A173V/M315I/H399Q/N429S/V466A, pH 7.2, 23°C | Haematococcus lacustris |
| 8.6 | - |
substrate: atrazine, D30G/Q71G/M315I/R389C/H399Q/N429S/V466A, pH 7.2, 23°C | Haematococcus lacustris |
| 8.7 | - |
substrate: atrazine, V12A/Q71T/T121A/A173V/M315I/H399Q/N429S/V466A, pH 7.2, 23°C | Haematococcus lacustris |
| 9.6 | - |
substrate: atrazine, D30G/G147D/G162E/A170V/H406P/V466A, pH 7.2, 23°C | Haematococcus lacustris |
| 10.3 | - |
substrate: atrazine, V12T/I393G/R389C/L395W/N429S/L446S/V466A, pH 7.2, 23°C | Haematococcus lacustris |
| 10.6 | - |
substrate: atrazine, D30G/I393P/L395S/N429S/V466A, pH 7.2, 23°C | Haematococcus lacustris |
| 10.7 | - |
substrate: atrazine, Q7R/V12A/S307P/M337T/I393R/L395R/S438G/R462W, pH 7.2, 23°C | Haematococcus lacustris |
| 12.2 | - |
substrate: atrazine, G55S/I393W/L395R/M315I/E433K, pH 7.2, 23°C | Haematococcus lacustris |
| 12.8 | - |
substrate: atrazine, V12P/D30G/Q71R/H249Y/270V/R389C/I393P/L395P, pH 7.2, 23°C | Haematococcus lacustris |
| 15.6 | - |
substrate: atrazine, V12A/Q71A/M315I/M341V, pH 7.2, 23°C | Haematococcus lacustris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| atrazine + H2O | - |
Haematococcus lacustris | hydroxyatrazine + chloride | - |
? | |
| atrazine + H2O | - |
Haematococcus lacustris | 2-hydroxyatrazine + chloride | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtzA | - |
Haematococcus lacustris |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.66 | - |
Atrazine | wild-type enzyme, pH 7.2, 23°C | Haematococcus lacustris | |
| 1.67 | - |
Atrazine | D30G/Q91M/T195A/N429S/M99T/R290H, pH 7.2, 23°C | Haematococcus lacustris | |
| 2.87 | - |
Atrazine | Q71R/V58A/R389C/I209M/E317G, pH 7.2, 23°C | Haematococcus lacustris | |
| 3.81 | - |
Atrazine | V12C/A139P/M315I/R389C/I393E/L395R/F439L, pH 7.2, 23°C | Haematococcus lacustris | |
| 3.86 | - |
Atrazine | Q71P/D128Y/I194V/T195A/M315I/R389C/H399Q/A470T, pH 7.2, 23°C | Haematococcus lacustris | |
| 4.06 | - |
Atrazine | D30G/Q71P/R271C/L288Q/M337T/R389C/H399Q/N429S, pH 7.2, 23°C | Haematococcus lacustris | |
| 4.28 | - |
Atrazine | V12A/Q71T/A173V/M315I/H399Q/N429S/V466A, pH 7.2, 23°C | Haematococcus lacustris | |
| 4.32 | - |
Atrazine | V12A/Q71T/M315I/H399Q/N429S/V466A, pH 7.2, 23°C | Haematococcus lacustris | |
| 4.73 | - |
Atrazine | V12A/Q71T/T121A/A173V/M315I/H399Q/N429S/V466A, pH 7.2, 23°C | Haematococcus lacustris | |
| 4.87 | - |
Atrazine | D30G/G147D/G162E/A170V/H406P/V466A, pH 7.2, 23°C | Haematococcus lacustris | |
| 5.86 | - |
Atrazine | V12T/I393G/R389C/L395W/N429S/L446S/V466A, pH 7.2, 23°C | Haematococcus lacustris | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Haematococcus lacustris |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11.1 | - |
Atrazine | wild-type enzyme, pH 7.2, 23°C | Haematococcus lacustris | |
| 15.2 | - |
Atrazine | D30G/Q91M/T195A/N429S/M99T/R290H, pH 7.2, 23°C | Haematococcus lacustris | |
| 28.8 | - |
Atrazine | V12A/Q71T/M315I/H399Q/N429S/V466A, pH 7.2, 23°C | Haematococcus lacustris | |
| 29 | - |
Atrazine | V12C/A139P/M315I/R389C/I393E/L395R/F439L, pH 7.2, 23°C | Haematococcus lacustris | |
| 29.1 | - |
Atrazine | D30G/Q71P/R271C/L288Q/M337T/R389C/H399Q/N429S, pH 7.2, 23°C | Haematococcus lacustris | |
| 29.2 | - |
Atrazine | D30G/Q71G/M315I/R389C/H399Q/N429S/V466A, pH 7.2, 23°C | Haematococcus lacustris | |
| 29.4 | - |
Atrazine | Q71P/D128Y/I194V/T195A/M315I/R389C/H399Q/A470T, pH 7.2, 23°C | Haematococcus lacustris | |
| 29.5 | - |
Atrazine | V12A/Q71T/A173V/M315I/H399Q/N429S/V466A, pH 7.2, 23°C | Haematococcus lacustris | |
| 30 | - |
Atrazine | V12F/D30G/V58A/T121A/I393S/L395H, pH 7.2, 23°C | Haematococcus lacustris | |
| 32 | - |
Atrazine | Q71R/V58A/R389C/I209M/E317G, pH 7.2, 23°C | Haematococcus lacustris | |
| 32.9 | - |
Atrazine | D30G/G147D/G162E/A170V/H406P/V466A, pH 7.2, 23°C | Haematococcus lacustris | |
| 33.2 | - |
Atrazine | V12A/Q71T/T121A/A173V/M315I/H399Q/N429S/V466A, pH 7.2, 23°C | Haematococcus lacustris | |
| 35.4 | - |
Atrazine | D30G/I393P/L395S/N429S/V466A, pH 7.2, 23°C | Haematococcus lacustris | |
| 36.2 | - |
Atrazine | Q7R/V12A/S307P/M337T/I393R/L395R/S438G/R462W, pH 7.2, 23°C | Haematococcus lacustris | |
| 36.5 | - |
Atrazine | G55S/I393W/L395R/M315I/E433K, pH 7.2, 23°C | Haematococcus lacustris | |
| 37.7 | - |
Atrazine | V12P/D30G/Q71R/H249Y/270V/R389C/I393P/L395P, pH 7.2, 23°C | Haematococcus lacustris | |
| 42 | - |
Atrazine | V12T/I393G/R389C/L395W/N429S/L446S/V466A, pH 7.2, 23°C | Haematococcus lacustris | |
| 43.6 | - |
Atrazine | V12A/Q71A/M315I/M341V, pH 7.2, 23°C | Haematococcus lacustris | |
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