Application | Comment | Organism |
---|---|---|
agriculture | degradation of chemically stable and toxic herbicide atrazine in soil and groundwater to non-toxic hydroxyatrazine | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.149 | - |
Atrazine | - |
Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal ions detectable with the enzym expressed in E. coli | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
245000 | - |
gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
atrazine + H2O | Pseudomonas sp. | - |
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.5 | - |
purified enzyme | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
atrazine + H2O | - |
Pseudomonas sp. | 4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl | - |
? | |
desethylatrazine + H2O | - |
Pseudomonas sp. | 2-hydroxy-4-amino-6-(isopropylamino)-1,3,5-triazine + HCl | - |
? | |
simazine + H2O | - |
Pseudomonas sp. | 4,6-diethylamino-1,3,5-triazine + HCl | - |
? |
Subunits | Comment | Organism |
---|---|---|
pentamer | 4 or 5 * 60000-52400, SDS-PAGE and calculation from DNA-sequence | Pseudomonas sp. |
tetramer | 4 or 5 * 60000-52400, SDS-PAGE and calculation from DNA-sequence | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
AtzA | - |
- |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
Atrazine | - |
Pseudomonas sp. |