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Literature summary for 3.8.1.7 extracted from

  • Löffler, F.; Lingens, F.; Muller, R.
    Dehalogenation of 4-chlorobenzoate. Characterisation of 4-chlorobenzoyl-coenzyme A dehalogenase from Pseudomonas sp. CBS3 (1995), Biodegradation, 6, 203-212.
    View publication on PubMed
Search for more literature for 3.8.1.7

General Stability

General Stability Organism
pure enzyme is completely inactive after a single freeze and thawing cycle Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
 Pseudomonas sp.
Ag+ 5 mM Pseudomonas sp.
Co2+ 5 mM Pseudomonas sp.
Cu2+ 5 mM Pseudomonas sp.
Fe2+ 5 mM Pseudomonas sp.
Mn2+ 5 mM Pseudomonas sp.
Ni2+ 5 mM Pseudomonas sp.
SDS 1 mM, 80% inhibition Pseudomonas sp.
Zn2+ 5 mM Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0024 0.0027 4-chlorobenzoyl-CoA
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12000
-
 gel filtration Pseudomonas sp.
31000
-
 4 * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE Pseudomonas sp.
31000
-
 8 * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE Pseudomonas sp.
31000
-
 x * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. CBS-3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.25
-
-
 Pseudomonas sp.

Storage Stability

Storage Stability Organism
-20°C, impure preparations, stable for several months Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-bromobenzoyl-CoA + H2O
-
 Pseudomonas sp. 4-hydroxybenzoyl-CoA + bromide
-
 ?
4-bromobenzoyl-CoA + H2O
-
 Pseudomonas sp. CBS-3 4-hydroxybenzoyl-CoA + bromide
-
 ?
4-chlorobenzoyl-CoA + H2O
-
 Pseudomonas sp. 4-hydroxybenzoyl-CoA + chloride
-
 ?
4-chlorobenzoyl-CoA + H2O
-
 Pseudomonas sp. CBS-3 4-hydroxybenzoyl-CoA + chloride
-
 ?
4-fluorobenzoyl-CoA + H2O no activity Pseudomonas sp. 4-hydroxybenzoyl-CoA + fluoride
-
 ?
4-fluorobenzoyl-CoA + H2O no activity Pseudomonas sp. CBS-3 4-hydroxybenzoyl-CoA + fluoride
-
 ?
4-iodobenzoyl-CoA + H2O
-
 Pseudomonas sp. 4-hydroxybenzoyl-CoA + iodide
-
 ?
4-iodobenzoyl-CoA + H2O
-
 Pseudomonas sp. CBS-3 4-hydroxybenzoyl-CoA + iodide
-
 ?

Subunits

Subunits Comment Organism
multimer x * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE Pseudomonas sp.
octamer 8 * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE Pseudomonas sp.
tetramer 4 * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Pseudomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 65 about 60% of maximal activity at 30°C and at 65°C Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 15 min, stable Pseudomonas sp.
60
-
 15 min, 30% loss of activity Pseudomonas sp.
65
-
 15 min, complete loss of activity Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5
-
 4-chlorobenzoyl-CoA
-
 Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
9 11.5 about 50% of maximal activity at pH 9.0 and at pH 11.5 Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
 10 min, complete loss of activity Pseudomonas sp.
6
-
 60 min, 10% loss of activity Pseudomonas sp.
6.5 11 60 min, stable Pseudomonas sp.
11.5
-
 60 min, 10% loss of activity Pseudomonas sp.
12
-
 10 min, complete loss of activity Pseudomonas sp.