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Literature summary for 3.8.1.5 extracted from
- Ylehd, an epoxide hydrolase with promiscuous haloalkane dehalogenase activity from tropical marine yeast Yarrowia lipolytica is induced upon xenobiotic stress (2017), Sci. Rep., 7, 11887 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 1-5 mM, activates | Yarrowia lipolytica |  |
| dithiothreitol | 1-5 mM, activates | Yarrowia lipolytica | |
| reduced glutathione | 1-5 mM, activates | Yarrowia lipolytica | |
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | potential biocatalyst for bioremediation/biosensing of mixed pollutants | Yarrowia lipolytica |
| environmental protection | potential biocatalyst for bioremediation/biosensing of mixed pollutants | Yarrowia lipolytica |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the recombinant His-tagged enzyme is expressed in Escherichia coli BL21AI | Yarrowia lipolytica |
General Stability
| General Stability | Organism |
|---|---|
| glycerol stabilizes the enzyme | Yarrowia lipolytica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag2+ | 1-5 mM, strong inhibition | Yarrowia lipolytica | |
| Co2+ | 1-5 mM, strong inhibition | Yarrowia lipolytica | |
| Cu2+ | 1-5 mM, strong inhibition | Yarrowia lipolytica | |
| Fe2+ | 1-5 mM, complete inhibition | Yarrowia lipolytica | |
| Fe3+ | 1-5 mM, complete inhibition | Yarrowia lipolytica | |
| Hg2+ | 1-5 mM, strong inhibition | Yarrowia lipolytica | |
| Mn2+ | 1-5 mM, strong inhibition | Yarrowia lipolytica | |
| Ni2+ | 1-5 mM, complete inhibition | Yarrowia lipolytica | |
| Pb2+ | 1-5 mM, strong inhibition | Yarrowia lipolytica | |
| Zn2+ | 1-5 mM, complete inhibition | Yarrowia lipolytica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.24 | - |
1-bromododecane | pH 4.5, 30°C | Yarrowia lipolytica | |
| 4.15 | - |
1-bromodecane | pH 4.5, 30°C | Yarrowia lipolytica | |
| 5.12 | - |
1-Bromopropane | pH 4.5, 30°C | Yarrowia lipolytica | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Yarrowia lipolytica | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
non-denaturing PAGE, SDS-PAGE | Yarrowia lipolytica |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Glycerol | stabilizes the enzyme | Yarrowia lipolytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Yarrowia lipolytica | Q6C598 | - |
- |
| Yarrowia lipolytica CLIB 122 | Q6C598 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Yarrowia lipolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-bromodecane + H2O | - |
Yarrowia lipolytica | 1-decanol + bromide | - |
? | |
| 1-bromodecane + H2O | - |
Yarrowia lipolytica CLIB 122 | 1-decanol + bromide | - |
? | |
| 1-bromododecane + H2O | - |
Yarrowia lipolytica | 1-dodecanol + bromide | - |
? | |
| 1-bromododecane + H2O | - |
Yarrowia lipolytica CLIB 122 | 1-dodecanol + bromide | - |
? | |
| 1-bromopropane + H2O | - |
Yarrowia lipolytica | 1-propanol + bromide | - |
? | |
| 1-bromopropane + H2O | - |
Yarrowia lipolytica CLIB 122 | 1-propanol + bromide | - |
? | |
| additional information | the bifunctioal enzyme also shows epoxidase activity | Yarrowia lipolytica | ? | - |
- |
|
| additional information | the bifunctioal enzyme also shows epoxidase activity | Yarrowia lipolytica CLIB 122 | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 43000, SDS-PAGE | Yarrowia lipolytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HLD | - |
Yarrowia lipolytica |
| protein XP_504164 | - |
Yarrowia lipolytica |
| Ylehd | - |
Yarrowia lipolytica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
1-bromodecane | pH 4.5, 30°C | Yarrowia lipolytica | |
| 0.16 | - |
1-bromododecane | pH 4.5, 30°C | Yarrowia lipolytica | |
| 1.57 | - |
1-Bromopropane | pH 4.5, 30°C | Yarrowia lipolytica | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Yarrowia lipolytica | the expression of Ylehd is highly induced in presence of 1-bromodecane and 1,2-epoxyoctane but not in glucose grown cells. Higher expression in presence of xenobiotics | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | xenobiotic stress mitigation | Yarrowia lipolytica |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.12 | - |
1-Bromopropane | pH 4.5, 30°C | Yarrowia lipolytica | |
| 8.8 | - |
1-bromododecane | pH 4.5, 30°C | Yarrowia lipolytica | |
| 10 | - |
1-bromodecane | pH 4.5, 30°C | Yarrowia lipolytica | |
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