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Literature summary for 3.8.1.5 extracted from

  • Fung, H.; Gadd, M.; Drury, T.; Cheung, S.; Guss, J.; Coleman, N.; Matthews, J.
    Biochemical and biophysical characterisation of haloalkane dehalogenases DmrA and DmrB in Mycobacterium strain JS60 and their role in growth on haloalkanes (2015), Mol. Microbiol., 97, 439-453 .
    View publication on PubMed
Search for more literature for 3.8.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycolicibacterium rhodesiae

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour diffusion method, crystal structure of selenomethionyl-DmrA is determined to 1.7 A resolution Mycolicibacterium rhodesiae
selenomethionyl-labelled enzyme DmrA, hanging drop vapor diffusion method, using 0.1 M bis-Tris propane, pH 6.5, 0.2 M KSCN, 16% (w/v) PEG 3350 Mycolicibacterium rhodesiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.9
-
 4-bromobutyronitrile at pH 8.0 and 25°C Mycolicibacterium rhodesiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 gel filtration Mycolicibacterium rhodesiae
150000
-
 gel filtration Mycolicibacterium rhodesiae

Organism

Organism UniProt Comment Textmining
Mycolicibacterium rhodesiae G4I2J6
-
-
Mycolicibacterium rhodesiae G4I5P8
-
-
Mycolicibacterium rhodesiae JS60 G4I2J6
-
-
Mycolicibacterium rhodesiae JS60 G4I5P8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycolicibacterium rhodesiae
Ni-NTA agarose column chromatography and Superdex 75 gel filtration Mycolicibacterium rhodesiae
Ni-NTA agarose column chromatography, UNO Q6 anion exchange column chromatography and Superdex 75 gel filtration Mycolicibacterium rhodesiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dibromoethane + H2O
-
 Mycolicibacterium rhodesiae 2-bromoethanol + bromide
-
 ?
1,2-dibromoethane + H2O about 45% of the specific activity compared to 1-bromohexane, enzyme form DmrB Mycolicibacterium rhodesiae 2-bromoethanol + bromide
-
 ?
1,2-dibromoethane + H2O about 90% of the specific activity compared to 1-bromobutane, enzyme form DmrA Mycolicibacterium rhodesiae 2-bromoethanol + bromide
-
 ?
1,2-dibromoethane + H2O strong dehalogenase activity Mycolicibacterium rhodesiae 2-bromoethanol + bromide
-
 ?
1,2-dibromoethane + H2O about 90% of the specific activity compared to 1-bromobutane, enzyme form DmrA Mycolicibacterium rhodesiae JS60 2-bromoethanol + bromide
-
 ?
1,2-dibromoethane + H2O
-
 Mycolicibacterium rhodesiae JS60 2-bromoethanol + bromide
-
 ?
1,2-dibromoethane + H2O about 45% of the specific activity compared to 1-bromohexane, enzyme form DmrB Mycolicibacterium rhodesiae JS60 2-bromoethanol + bromide
-
 ?
1,2-dibromohexane + H2O
-
 Mycolicibacterium rhodesiae 2-bromohexanol + bromide
-
 ?
1,2-dichloroethane + H2O low activity Mycolicibacterium rhodesiae 2-chloroethanol + chloride
-
 ?
1,2-dichloroethane + H2O low activity Mycolicibacterium rhodesiae JS60 2-chloroethanol + chloride
-
 ?
1,6-dibromohexane + H2O
-
 Mycolicibacterium rhodesiae 6-bromohexanol + bromide
-
 ?
1,6-dibromohexane + H2O about 25% of the specific activity compared to 1-bromobutane, enzyme form DmrA Mycolicibacterium rhodesiae 6-bromohexanol + bromide
-
 ?
1,6-dibromohexane + H2O about 45% of the specific activity compared to 1-bromohexane, enzyme form DmrB Mycolicibacterium rhodesiae 6-bromohexanol + bromide
-
 ?
1,6-dibromohexane + H2O about 45% of the specific activity compared to 1-bromohexane, enzyme form DmrB Mycolicibacterium rhodesiae JS60 6-bromohexanol + bromide
-
 ?
1-bromobutane + H2O
-
 Mycolicibacterium rhodesiae 1-butanol + bromide
-
 ?
1-bromobutane + H2O strong dehalogenase activity Mycolicibacterium rhodesiae 1-butanol + bromide
-
 ?
1-bromohexane + H2O
-
 Mycolicibacterium rhodesiae 1-hexanol + bromide
-
 ?
1-bromohexane + H2O about 35% of the specific activity compared to 1-bromobutane, enzyme form DmrA Mycolicibacterium rhodesiae 1-hexanol + bromide
-
 ?
1-bromohexane + H2O clearly preferred substrate Mycolicibacterium rhodesiae 1-hexanol + bromide
-
 ?
1-bromohexane + H2O enzyme form DmrB Mycolicibacterium rhodesiae 1-hexanol + bromide
-
 ?
1-bromopentane + H2O
-
 Mycolicibacterium rhodesiae 1-pentanol + bromide
-
 ?
1-bromopentane + H2O about 15% of the specific activity compared to 1-bromohexane, enzyme form DmrB Mycolicibacterium rhodesiae 1-pentanol + bromide
-
 ?
1-bromopentane + H2O about 85% of the specific activity compared to 1-bromobutane, enzyme form DmrA Mycolicibacterium rhodesiae 1-pentanol + bromide
-
 ?
1-bromopentane + H2O strong dehalogenase activity Mycolicibacterium rhodesiae 1-pentanol + bromide
-
 ?
1-bromopropane + H2O
-
 Mycolicibacterium rhodesiae 1-propanol + bromide
-
 ?
1-bromopropane + H2O about 60% of the specific activity compared to 1-bromobutane, enzyme form DmrA Mycolicibacterium rhodesiae 1-propanol + bromide
-
 ?
1-iodobutane + H2O
-
 Mycolicibacterium rhodesiae 1-butanol + iodide
-
 ?
1-iodobutane + H2O about 50% of the specific activity compared to 1-bromobutane, enzyme form DmrA Mycolicibacterium rhodesiae 1-butanol + iodide
-
 ?
1-iodopropane + H2O
-
 Mycolicibacterium rhodesiae 1-propanol + iodide
-
 ?
1-iodopropane + H2O about 50% of the specific activity compared to 1-bromobutane, enzyme form DmrA Mycolicibacterium rhodesiae 1-propanol + iodide
-
 ?
4-bromobutyronitrile + H2O
-
 Mycolicibacterium rhodesiae 4-hydroxybutyronitrile + bromide
-
 ?
4-bromobutyronitrile + H2O strong dehalogenase activity Mycolicibacterium rhodesiae 4-hydroxybutyronitrile + bromide
-
 ?
4-bromobutyronitrile + H2O about 20% of the specific activity compared to 1-bromohexane, enzyme form DmrB Mycolicibacterium rhodesiae ? + bromide
-
 ?
4-bromobutyronitrile + H2O about 85% of the specific activity compared to 1-bromobutane, enzyme form DmrA Mycolicibacterium rhodesiae ? + bromide
-
 ?
bromocyclohexane + H2O low activity Mycolicibacterium rhodesiae cyclohexanol + bromide
-
 ?
additional information active against C2-C6 haloalkanes, with a preference for brominated linear substrates Mycolicibacterium rhodesiae ?
-
-
additional information active against C2-C6 haloalkanes, with a preference for brominated linear substrates, enzyme form DmrA Mycolicibacterium rhodesiae ?
-
-
additional information enzyme DmrA is active against mono- and dihalogenated alkanes up to six carbons in length and favours brominated species over iodinated or chlorinated ones and linear substrates over cyclic ones Mycolicibacterium rhodesiae ?
-
-
additional information no activity with chloroform Mycolicibacterium rhodesiae ?
-
-
additional information no activity with chloroform and 1,1,2-trichloroethane Mycolicibacterium rhodesiae ?
-
-
additional information active against C2-C6 haloalkanes, with a preference for brominated linear substrates, enzyme form DmrA Mycolicibacterium rhodesiae JS60 ?
-
-
additional information enzyme DmrA is active against mono- and dihalogenated alkanes up to six carbons in length and favours brominated species over iodinated or chlorinated ones and linear substrates over cyclic ones Mycolicibacterium rhodesiae JS60 ?
-
-
additional information no activity with chloroform and 1,1,2-trichloroethane Mycolicibacterium rhodesiae JS60 ?
-
-
additional information active against C2-C6 haloalkanes, with a preference for brominated linear substrates Mycolicibacterium rhodesiae JS60 ?
-
-
additional information no activity with chloroform Mycolicibacterium rhodesiae JS60 ?
-
-

Subunits

Subunits Comment Organism
homotetramer 4 * 37100, multi-angle laser-light scattering analysis Mycolicibacterium rhodesiae
monomer 1 * 35500, multi-angle laser-light scattering analysis Mycolicibacterium rhodesiae
monomer DmrA Mycolicibacterium rhodesiae
tetramer DmrB Mycolicibacterium rhodesiae

Synonyms

Synonyms Comment Organism
DmrA
-
 Mycolicibacterium rhodesiae
DmrB
-
 Mycolicibacterium rhodesiae
HLD
-
 Mycolicibacterium rhodesiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
-
 Mycolicibacterium rhodesiae
38
-
 substrate: 1-bromobutane Mycolicibacterium rhodesiae
38
-
 substrate: 1-bromohexane Mycolicibacterium rhodesiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1
-
 4-bromobutyronitrile at pH 8.0 and 25°C Mycolicibacterium rhodesiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Mycolicibacterium rhodesiae
8
-
 substrate: 1-bromohexane Mycolicibacterium rhodesiae
8.5
-
-
 Mycolicibacterium rhodesiae
8.5
-
 substrate: 1-bromobutane Mycolicibacterium rhodesiae

Expression

Organism Comment Expression
Mycolicibacterium rhodesiae DmrA shows significantly higher levels of upregulation in response to haloalkanes than DmrB up