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Literature summary for 3.8.1.5 extracted from

  • Kotik, M.; Vanacek, P.; Kunka, A.; Prokop, Z.; Damborsky, J.
    Metagenome-derived haloalkane dehalogenases with novel catalytic properties (2017), Appl. Microbiol. Biotechnol., 101, 6385-6397 .
    View publication on PubMed
Search for more literature for 3.8.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Star (DE3) cells uncultured bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37400
-
 gel filtration, enzyme form eHLD-C uncultured bacterium
37900
-
 gel filtration, enzyme form eHLD-B uncultured bacterium

Organism

Organism UniProt Comment Textmining
uncultured bacterium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 uncultured bacterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.86
-
 substrate: 1,3-dibromopropane, enzyme form eHLD-B, pH and temperature not specified in the publication uncultured bacterium
12.2
-
 substrate: 1,2-dibromoethane, enzyme form eHLD-C, pH and temperature not specified in the publication uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1-bromomethyl)cyclohexane + H2O 2.5% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B uncultured bacterium ?
-
 ?
(1-bromomethyl)cyclohexane + H2O less than 1% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium ?
-
 ?
1,2,3-tribromopropane + H2O about 50% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium 2,3-dibromo-1-propanol + bromide
-
 ?
1,2-dibromo-3-chloropropane + H2O about 40% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium 2-bromo-3-chloro-1-propanol + bromide
-
 ?
1,2-dibromo-3-chloropropane + H2O about 5% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B uncultured bacterium 2-bromo-3-chloro-1-propanol + bromide
-
 ?
1,2-dibromoethane + H2O about 25% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B uncultured bacterium 2-bromo-1-ethanol + bromide
-
 ?
1,2-dibromoethane + H2O the highest specific activity of enzyme form eHLD-C is determined for 1,2-dibromoethane and 1-bromo-2-chloroethane uncultured bacterium 2-bromo-1-ethanol + bromide
-
 ?
1,2-dibromopropane + H2O about 65% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium 2-bromo-1-propanol + bromide
-
 ?
1,2-dichloroethane + H2O less than 1% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium 2-chloro-1-ethanol + chloride
-
 ?
1,2-dichloropropane + H2O less than 1% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium 2-chloro-1-propanol + chloride
-
 ?
1,3-dibromopropane + H2O about 40% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium 3-bromo-1-propanol + bromide
-
 ?
1,3-dibromopropane + H2O the highest specific activity of enzyme form eHLD-B is determined for 1,3-dibromopropane and 1-bromo-3-chloropropane uncultured bacterium 3-bromo-1-propanol + bromide
-
 ?
1,3-dichloropropane + H2O about 10% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium 3-chloro-1-propanol + chloride
-
 ?
1,3-diiodopropane + H2O 4% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium 3-iodo-1-propanol + iodide
-
 ?
1,3-diiodopropane + H2O about 35% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B uncultured bacterium 3-iodo-1-propanol + iodide
-
 ?
1,5-dichloropentane + H2O 3% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium 5-chloro-1-pentanol + chloride
-
 ?
1,5-dichloropentane + H2O about 45% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B uncultured bacterium 5-chloro-1-pentanol + chloride
-
 ?
1-bromo-2-chloroethane + H2O about 10% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B uncultured bacterium 2-chloro-1-ethanol + bromide
-
 ?
1-bromo-2-chloroethane + H2O about 85% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium 2-chloro-1-ethanol + bromide
-
 ?
1-bromo-3-chloropropane + H2O about 75% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium 3-chloro-1-propanol + bromide
-
 ?
1-bromo-3-chloropropane + H2O about 80% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B uncultured bacterium 3-chloro-1-propanol + bromide
-
 ?
1-bromobutane + H2O about 35% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium 1-butanol + bromide
-
 ?
1-bromobutane + H2O about 60% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B uncultured bacterium 1-butanol + bromide
-
 ?
1-bromohexane + H2O 4% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium 1-hexanol + bromide
-
 ?
1-bromohexane + H2O about 20% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B uncultured bacterium 1-hexanol + bromide
-
 ?
1-chlorobutane + H2O less than 1% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium 1-butanol + chloride
-
 ?
1-chlorohexane + H2O about 20% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B uncultured bacterium 1-hexanol + chloride
-
 ?
1-chlorohexane + H2O less than 1% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium 1-hexanol + chloride
-
 ?
1-iodobutane + H2O 4% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium 1-butanol + iodide
-
 ?
1-iodobutane + H2O about 25% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B uncultured bacterium 1-butanol + iodide
-
 ?
1-iodohexane + H2O 2.5% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B uncultured bacterium 1-hexanol + iodide
-
 ?
1-iodohexane + H2O less than 1% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium 1-hexanol + iodide
-
 ?
1-iodopropane + H2O 5% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium 1-propanol + iodide
-
 ?
1-iodopropane + H2O about 15% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B uncultured bacterium 1-propanol + iodide
-
 ?
2,3-dichloropropene + H2O about 15% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium ?
-
 ?
2,3-dichloropropene + H2O about 5% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B uncultured bacterium ?
-
 ?
2-bromo-1-chloropropane + H2O about 50% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B uncultured bacterium 2-bromo-1-propanol + chloride
-
 ?
2-bromo-1-chloropropane + H2O about 60% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium 2-bromo-1-propanol + chloride
-
 ?
2-iodobutane + H2O 3% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium 2-butanol + iodide
-
 ?
2-iodobutane + H2O 7.5% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B uncultured bacterium 2-butanol + iodide
-
 ?
3-chloro-2-methylpropene + H2O about 15% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium ?
-
 ?
3-chloro-2-methylpropene + H2O about 15% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B uncultured bacterium ?
-
 ?
4-bromobutyronitrile + H2O about 25% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium 4-hydroxybutyronitrile + bromide
-
 ?
4-bromobutyronitrile + H2O about 75% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B uncultured bacterium 4-hydroxybutyronitrile + bromide
-
 ?
bis(2-chloroethyl)ether + H2O 4% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium ?
-
 ?
bromocyclohexane + H2O 4% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium cyclohexanol + bromide
-
 ?
chlorocyclopentane + H2O about 10% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C uncultured bacterium cyclopentanol + chloride
-
 ?

Synonyms

Synonyms Comment Organism
eHLD-B
-
 uncultured bacterium
eHLD-C
-
 uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 at pH 8.5, enzyme form eHLD-B uncultured bacterium
55
-
 at pH 6.5, enzyme form eHLD-C uncultured bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
34 35.1 melting temperature, enzyme form eHLD-B uncultured bacterium
55.5 58.7 melting temperature, enzyme form eHLD-C uncultured bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 8.9 broad optimum with a plateau region of maximum activity uncultured bacterium
8.5
-
 enzyme form eHLD-B uncultured bacterium

pH Range

pH Minimum pH Maximum Comment Organism
4.7 10 pH 4.7: about % of maximal activity, pH 10.0: about % of maximal activity, enzyme form eHLD-C uncultured bacterium
6 9.7 pH 6.0: about % of maximal activity, pH 9.7: about % of maximal activity, enzyme form eHLD-B uncultured bacterium