Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.8.1.5 extracted from

  • Buryska, T.; Babkova, P.; Vavra, O.; Damborsky, J.; Prokop, Z.
    A haloalkane dehalogenase from a marine microbial consortium possessing exceptionally broad substrate specificity (2018), Appl. Environ. Microbiol., 84, e01684-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.8.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Lyngbya majuscula

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.38
-
 1-Chlorobutane 37°C, pH 8.6 Lyngbya majuscula
0.43
-
 1-Iodobutane 37°C, pH 8.6 Lyngbya majuscula
0.86
-
 1-Iodopropane 37°C, pH 8.6 Lyngbya majuscula
0.9
-
 4-bromobutyronitrile 37°C, pH 8.6 Lyngbya majuscula
2.3
-
 1,2-Dibromoethane 37°C, pH 8.6 Lyngbya majuscula

Organism

Organism UniProt Comment Textmining
Lyngbya majuscula Q6DND9
-
-

Purification (Commentary)

Purification (Comment) Organism
metallo-affinity chromatography Lyngbya majuscula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2,3-tribromopropane + H2O about 10% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 2,3-dibromo-1-propanol + bromide
-
 ?
1,2-dibromo-3-chloropropane about 35% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 2-bromo-3-chloro-1-propanol + bromide
-
 ?
1,2-dibromoethane + H2O about 45% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 2-bromoethanol + bromide
-
 ?
1,2-dibromopropane + H2O about 15% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 2-bromo-1-propanol + bromide
-
 ?
1,3-dibromobutane + H2O about 20% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 3-bromo-1-propanol + bromide
-
 ?
1,3-diiodopropane + H2O about 25% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 3-iodo-1-propanol + iodide
-
 ?
1-bromo-2-chloroethane about 50% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 2-chloro-1-ethanol + bromide
-
 ?
1-bromo-3-chloropropane + H2O about 25% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 3-chloro-1-propanol + bromide
-
 ?
1-bromobutane + H2O about 10% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 1-butanol + bromide
-
 ?
1-chlorobutane + H2O less than 5% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 1-butanol + chloride
-
 ?
1-iodobutane + H2O about 5% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 1-butanol + iodide
-
 ?
1-iodopropane + H2O about 10% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 1-propanol + iodide
-
 ?
2,3-dichloroprop-1-ene + H2O about 15% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula ?
-
 ?
3-chloro-2-methylprop-1-ene + H2O about 5% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula ?
-
 ?
4-bromobutyronitrile + H2O best substrate Lyngbya majuscula 4-hydroxybutyronitrile + bromide
-
 ?
bromocyclohexane + H2O about 20% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula cyclohexanol + bromide
-
 ?
chlorocyclopentane + H2O about 10% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula cyclopentanol + chloride
-
 ?
additional information the enzyme prefers brominated substrates over chlorinated and iodinated substrates. Moderate to high enantioselectivity with a preference for the (R) enantiomer is observed for brominated esters with the highest E value of 106 achieved with ethyl-2-bromopropionate. Negligible enantioselectivity is observed with either of the tested brominated n-alkanes Lyngbya majuscula ?
-
-

Synonyms

Synonyms Comment Organism
DmmA
-
 Lyngbya majuscula

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Lyngbya majuscula

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 45 20°C: about 30% of maximal activity, 45°C: about 40% of maximal activity Lyngbya majuscula

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 half-life: 3.0 h Lyngbya majuscula

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.17
-
 1-Chlorobutane 37°C, pH 8.6 Lyngbya majuscula
0.21
-
 1-Iodobutane 37°C, pH 8.6 Lyngbya majuscula
0.43
-
 1-Iodopropane 37°C, pH 8.6 Lyngbya majuscula
1.2
-
 4-bromobutyronitrile 37°C, pH 8.6 Lyngbya majuscula
1.8
-
 1,2-Dibromoethane 37°C, pH 8.6 Lyngbya majuscula

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7
-
-
 Lyngbya majuscula

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.2 at least 90% of maximal activity within a pH range of 7.5 to 9.2 and more than 20% at pH values of 6.2 to 11.5 Lyngbya majuscula

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.45
-
 1-Chlorobutane 37°C, pH 8.6 Lyngbya majuscula
0.49
-
 1-Iodobutane 37°C, pH 8.6 Lyngbya majuscula
0.5
-
 1-Iodopropane 37°C, pH 8.6 Lyngbya majuscula
0.8
-
 1,2-Dibromoethane 37°C, pH 8.6 Lyngbya majuscula
1.3
-
 4-bromobutyronitrile 37°C, pH 8.6 Lyngbya majuscula