Application | Comment | Organism |
---|---|---|
synthesis | enzyme DspA is very enantioselective towards 2-bromobutane and may be attractive for biocatalysis | Strongylocentrotus purpuratus |
Cloned (Comment) | Organism |
---|---|
gene dspA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Strongylocentrotus purpuratus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetic analysis, kinetic resolution of 2-bromobutane and 2-bromopentane | Strongylocentrotus purpuratus | |
0.92 | - |
1,3-dibromopropane | pH 8.6, 37°C, recombinant His-tagged enzyme | Strongylocentrotus purpuratus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
DspA, with an expected molecular mass of 36 kDa, existed as a dimer in the absence of the salt, but is predominantly monomeric in the presence of 150 mM NaCl, native PAGE and gel filtration | Strongylocentrotus purpuratus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Strongylocentrotus purpuratus | the enzyme shows a preference for terminally brominated hydrocarbons and enantioselectivity towards beta-brominated alkanes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Strongylocentrotus purpuratus | - |
gene dspA | - |
Purification (Comment) | Organism |
---|---|
gene dspA, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity | Strongylocentrotus purpuratus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(bromomethyl)cyclohexane + H2O | - |
Strongylocentrotus purpuratus | cyclohexylmethanol + bromide | - |
? | |
1,2,3-tribromopropane + H2O | - |
Strongylocentrotus purpuratus | 2,3-dibromopropanol + bromide | - |
? | |
1,2-dibromo-3-chloropropane + H2O | - |
Strongylocentrotus purpuratus | 2-bromo-3-chloropropanol + bromide | - |
? | |
1,2-dibromoethane + H2O | - |
Strongylocentrotus purpuratus | 2-bromoethanol + bromide | - |
? | |
1,2-dibromopropane + H2O | - |
Strongylocentrotus purpuratus | 2-bromopropanol + bromide | - |
? | |
1,3-dibromopropane + H2O | high activity | Strongylocentrotus purpuratus | 3-bromopropanol + bromide | - |
? | |
1,3-diiodopropane + H2O | - |
Strongylocentrotus purpuratus | 3-iodopropanol + iodide | - |
? | |
1,5-dichloropentane + H2O | - |
Strongylocentrotus purpuratus | 5-chloropentanol + chloride | - |
? | |
1-bromo-2-chloroethane + H2O | - |
Strongylocentrotus purpuratus | 2-chloroethanol + bromide | - |
? | |
1-bromo-2-chloroethane + H2O | high activity | Strongylocentrotus purpuratus | 2-chloroethanol + bromide | - |
? | |
1-bromo-3-chloropropane + H2O | - |
Strongylocentrotus purpuratus | 3-chloropropanol + bromide | - |
? | |
1-bromobutane + H2O | - |
Strongylocentrotus purpuratus | 1-butanol + bromide | - |
? | |
1-bromohexane + H2O | best substrate | Strongylocentrotus purpuratus | 1-hexanol + bromide | - |
? | |
1-chloro-2-bromoethane + H2O | - |
Strongylocentrotus purpuratus | 2-bromoethanol + chloride | - |
? | |
1-iodobutane + H2O | - |
Strongylocentrotus purpuratus | butanol + iodide | - |
? | |
1-iodohexane + H2O | - |
Strongylocentrotus purpuratus | hexanol + iodide | - |
? | |
1-iodopropane + H2O | - |
Strongylocentrotus purpuratus | propanol + iodide | - |
? | |
2,3-dichloropropene + H2O | - |
Strongylocentrotus purpuratus | ? | - |
? | |
2-iodobutane + H2O | - |
Strongylocentrotus purpuratus | ? | - |
? | |
3-chloro-2-methylprop-1-ene + H2O | - |
Strongylocentrotus purpuratus | ? | - |
? | |
4-bromobutyronitrile + H2O | - |
Strongylocentrotus purpuratus | ? | - |
? | |
chlorocyclopentane + H2O | - |
Strongylocentrotus purpuratus | cyclopentanol + chloride | - |
? | |
additional information | the enzyme shows a preference for terminally brominated hydrocarbons and enantioselectivity towards beta-brominated alkanes | Strongylocentrotus purpuratus | ? | - |
? | |
additional information | substrate specificity profile of DspA, overview. No activity with 1-chlorobutane, 1-chlorohexane, 1,2-dichloroethane, 1,3-dichloropropane, 1,2-dichloropropane, 1,2,3-trichloropropane, bis(2-chlorethyl)ether, chlorocyclohexane, and bromocyclohexane. Medium enantioselectivity is observed with 2-bromobutane and 2-bromopentane, while none or very low enantioselectivity wi observed with methyl 2-bromobutyrate and ethyl 2-bromobutyrate | Strongylocentrotus purpuratus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36000, DspA, with an expected molecular mass of 36 kDa, existed as a dimer in the absence of the salt, but is predominantly monomeric in the presence of 150 mM NaCl, SDS-PAGE | Strongylocentrotus purpuratus |
monomer | 1 * 36000, DspA, with an expected molecular mass of 36 kDa, existed as a dimer in the absence of the salt, but is predominantly monomeric in the presence of 150 mM NaCl, SDS-PAGE | Strongylocentrotus purpuratus |
Synonyms | Comment | Organism |
---|---|---|
DspA | - |
Strongylocentrotus purpuratus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Strongylocentrotus purpuratus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.57 | - |
1,3-dibromopropane | pH 8.6, 37°C, recombinant His-tagged enzyme | Strongylocentrotus purpuratus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
assay at | Strongylocentrotus purpuratus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily | Strongylocentrotus purpuratus |