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Literature summary for 3.8.1.5 extracted from

  • Nagata, Y.; Ohtsubo, Y.; Tsuda, M.
    Properties and biotechnological applications of natural and engineered haloalkane dehalogenases (2015), Appl. Microbiol. Biotechnol., 99, 9865-9881.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
phylogenetic analysis and tree Corynebacterium sp.
phylogenetic analysis and tree Arthrobacter sp.
phylogenetic analysis and tree Rhodococcus sp.
phylogenetic analysis and tree Strongylocentrotus purpuratus
phylogenetic analysis and tree Sphingomonas sp.
phylogenetic analysis and tree Mycobacterium tuberculosis variant bovis
phylogenetic analysis and tree Sphingobium indicum
phylogenetic analysis and tree Xanthobacter autotrophicus
phylogenetic analysis and tree Bradyrhizobium japonicum
phylogenetic analysis and tree Mesorhizobium loti
phylogenetic analysis and tree Sphingobium sp.
phylogenetic analysis and tree Mycobacterium tuberculosis
phylogenetic analysis and tree Rhodococcus rhodochrous
phylogenetic analysis and tree Agrobacterium tumefaciens
phylogenetic analysis and tree Plesiocystis pacifica
phylogenetic analysis and tree Alcanivorax dieselolei
phylogenetic analysis and tree Mycobacterium sp.
phylogenetic analysis and tree Bradyrhizobium elkanii
phylogenetic analysis and tree Mycobacterium avium
phylogenetic analysis and tree Rhodopirellula baltica
phylogenetic analysis and tree Marinobacter sp.
phylogenetic analysis and tree Psychrobacter cryohalolentis
phylogenetic analysis and tree Rhodobacteraceae bacterium UDC319

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure determination and analysis Marinobacter sp.
crystal structure determination and analysis, PDB ID 1CIJ Xanthobacter autotrophicus
crystal structure determination and analysis, PDB ID 1CIJ Rhodobacteraceae bacterium UDC319
crystal structure determination and analysis, PDB ID 1CQW Rhodococcus rhodochrous
crystal structure determination and analysis, PDB ID 1CV2/2BFN Sphingobium indicum
crystal structure determination and analysis, PDB ID 2QVB Mycobacterium tuberculosis
crystal structure determination and analysis, PDB ID 2XT0 Plesiocystis pacifica
crystal structure determination and analysis, PDB ID 3WI7 for the wild-type and PDB ID 3WIB for the mutant enzyme Agrobacterium tumefaciens
crystal structure determination and analysis, PDB ID 4H77 Sphingobium sp.
crystal structure determination and analysis, PDB ID 4K2A Bradyrhizobium elkanii
crystal structure determination and analysis, PDB IDs 3A2M and 3AFI for te wild-type, and PDB ID 3A2L for the mutant Bradyrhizobium japonicum
crystal structure determination and analysis, PDB IDs 4BRZ and 4C6H Psychrobacter cryohalolentis
mutant crystal structure determination and analysis, PDB IDs 4FWB and 3SK0 Mycobacterium sp.

Protein Variants

Protein Variants Comment Organism
C176Y/Y273F site-directed mutagenesis, the mutant enzyme is 3.5fold higher activity toward TCP than DhaA wild-type Mycobacterium sp.
H139A site-directed mutagenesis Bradyrhizobium japonicum
additional information construction of deletion mutant DbeADELTACl, mutation in the second halide-binding site Bradyrhizobium elkanii
additional information construction of deletion mutants DbjADELTA and DbjADELTA H139A Bradyrhizobium japonicum
additional information construction of engineered enzyme variants: the more thermostable Dhla5/Dhla8, the more active DhaAM2(C176Y/Y273F), the DhaA31 mutant that is 32fold higher activity toward TCP than DhaA wild-type, te mutant DhaA12 mimicking DbjA active (insertion of ERB), and the DhaA31 mutant that has higher enantioselectivity toward TCP than the wild-type Mycobacterium sp.
additional information construction of LinB variants, that mimick the DmtA active site, shows higher activity toward beta- and delta-HCH than LinBMI. Mutant 4H7D/4H7E/4H7F/4H7H/4H7I/4H7J/4H7K are intermediates between LinBUT and LinBMI Sphingobium sp.
additional information construction of LinBUT variants, 4H7D/4H7E/4H7F/4H7H/4H7I/4H7J/4H7K are intermediates between LinBUT and LinBMI Sphingobium indicum
additional information engineered enzyme variants are constructed for in vivo evolution for activity toward 1-chlorohexane, more activity toward 1,6-dichlorohexane than DhlA wild-type, and increased activity toward 1,2-dibromoethane/1-bromobutane the wild-type Xanthobacter autotrophicus
Y109W site-directed mutagenesis, mutant DatA01 Agrobacterium tumefaciens

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens E2RV69 gene datA
-
Agrobacterium tumefaciens C58 / ATCC 33970 E2RV69 gene datA
-
Alcanivorax dieselolei
-
-
-
Alcanivorax dieselolei B-5
-
-
-
Arthrobacter sp.
-
gene dhaA
-
Bradyrhizobium elkanii E2RV62
-
-
Bradyrhizobium elkanii USDA94 E2RV62
-
-
Bradyrhizobium japonicum P59337
-
-
Bradyrhizobium japonicum USDA 110 P59337
-
-
Corynebacterium sp.
-
gene dhaA
-
Marinobacter sp. A3JB27 a psychrophilic organism
-
Mesorhizobium loti Q98C03
-
-
Mesorhizobium loti MAFF303099 Q98C03
-
-
Mycobacterium avium Q93K00 gene dhmA
-
Mycobacterium avium N85 Q93K00 gene dhmA
-
Mycobacterium sp. Q9ZER0 gene dhaAF
-
Mycobacterium sp. GP1 Q9ZER0 gene dhaAF
-
Mycobacterium tuberculosis P9WMR9 dhaA
-
Mycobacterium tuberculosis H37Rv P9WMR9 dhaA
-
Mycobacterium tuberculosis variant bovis A4Q9R7 gene dmbC
-
Mycobacterium tuberculosis variant bovis Q6EUU8 gene dmbB
-
Mycobacterium tuberculosis variant bovis Q9XB14 gene dhaA
-
Mycobacterium tuberculosis variant bovis 5033/66 A4Q9R7 gene dmbC
-
Mycobacterium tuberculosis variant bovis 5033/66 Q6EUU8 gene dmbB
-
Mycobacterium tuberculosis variant bovis 5033/66 Q9XB14 gene dhaA
-
Plesiocystis pacifica A6G7B1
-
-
Psychrobacter cryohalolentis Q1QBB9 gene dhmA, a psychrophilic organism
-
Psychrobacter cryohalolentis K5 Q1QBB9 gene dhmA, a psychrophilic organism
-
Rhodobacteraceae bacterium UDC319 A0A023I2Y1
-
-
Rhodococcus rhodochrous P0A3G2 gene dhaA
-
Rhodococcus rhodochrous NCIMB 13064 P0A3G2 gene dhaA
-
Rhodococcus sp.
-
gene dhaA
-
Rhodopirellula baltica G3XCP3 gene drbA
-
Rhodopirellula baltica SH1 G3XCP3 gene drbA
-
Sphingobium indicum
-
-
-
Sphingobium indicum P51698 gene linB
-
Sphingobium indicum B90A
-
-
-
Sphingobium indicum UT26 P51698 gene linB
-
Sphingobium sp. A4PEU6
-
-
Sphingobium sp. MI1205 A4PEU6
-
-
Sphingomonas sp.
-
-
-
Sphingomonas sp. BHC-A
-
-
-
Strongylocentrotus purpuratus
-
-
-
Xanthobacter autotrophicus P22643 gene dhlA
-
Xanthobacter autotrophicus GJ10 P22643 gene dhlA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with chlorinated compounds Plesiocystis pacifica ?
-
?

Synonyms

Synonyms Comment Organism
DadB
-
Alcanivorax dieselolei
DatA
-
Agrobacterium tumefaciens
DbeA
-
Bradyrhizobium elkanii
DbjA
-
Bradyrhizobium japonicum
DhaA
-
Corynebacterium sp.
DhaA
-
Arthrobacter sp.
DhaA
-
Rhodococcus sp.
DhaA
-
Mycobacterium tuberculosis variant bovis
DhaA
-
Mycobacterium tuberculosis
DhaA
-
Rhodococcus rhodochrous
DhaB
-
Mycobacterium tuberculosis variant bovis
DhaC
-
Mycobacterium tuberculosis variant bovis
DhAf
-
Mycobacterium sp.
DhlA
-
Xanthobacter autotrophicus
DhmA
-
Mycobacterium avium
DhmA
-
Psychrobacter cryohalolentis
DmaA
-
Marinobacter sp.
dmbA
-
Mycobacterium tuberculosis variant bovis
DmbB
-
Mycobacterium tuberculosis variant bovis
DmbC
-
Mycobacterium tuberculosis variant bovis
dmlA
-
Mesorhizobium loti
DmtA
-
Mycobacterium tuberculosis
DmxA
-
Marinobacter sp.
DpcA
-
Psychrobacter cryohalolentis
DppA
-
Plesiocystis pacifica
DrbA
-
Rhodopirellula baltica
DspA
-
Strongylocentrotus purpuratus
HanR
-
Rhodobacteraceae bacterium UDC319
HLD
-
Corynebacterium sp.
HLD
-
Arthrobacter sp.
HLD
-
Rhodococcus sp.
HLD
-
Strongylocentrotus purpuratus
HLD
-
Sphingomonas sp.
HLD
-
Mycobacterium tuberculosis variant bovis
HLD
-
Sphingobium indicum
HLD
-
Xanthobacter autotrophicus
HLD
-
Bradyrhizobium japonicum
HLD
-
Mesorhizobium loti
HLD
-
Sphingobium sp.
HLD
-
Mycobacterium tuberculosis
HLD
-
Rhodococcus rhodochrous
HLD
-
Agrobacterium tumefaciens
HLD
-
Plesiocystis pacifica
HLD
-
Alcanivorax dieselolei
HLD
-
Mycobacterium sp.
HLD
-
Bradyrhizobium elkanii
HLD
-
Mycobacterium avium
HLD
-
Rhodopirellula baltica
HLD
-
Marinobacter sp.
HLD
-
Psychrobacter cryohalolentis
HLD
-
Rhodobacteraceae bacterium UDC319
LinBMI
-
Sphingomonas sp.
LinBMI
-
Sphingobium indicum
LinBMI
-
Sphingobium sp.
LinBUT
-
Sphingobium indicum
Rv2579
-
Mycobacterium tuberculosis

General Information

General Information Comment Organism
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup I of HLDs Xanthobacter autotrophicus
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup I of HLDs Plesiocystis pacifica
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup I of HLDs Mycobacterium tuberculosis variant bovis
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup I of HLDs Mycobacterium avium
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup I of HLDs Psychrobacter cryohalolentis
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs Corynebacterium sp.
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs Arthrobacter sp.
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs Rhodococcus sp.
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs Strongylocentrotus purpuratus
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs Sphingomonas sp.
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs Sphingobium indicum
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs Bradyrhizobium japonicum
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs Mesorhizobium loti
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs Sphingobium sp.
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs Rhodococcus rhodochrous
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs Agrobacterium tumefaciens
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs Alcanivorax dieselolei
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs Mycobacterium sp.
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs Bradyrhizobium elkanii
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs Marinobacter sp.
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs Rhodobacteraceae bacterium UDC319
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs. Q293 is the single amino acid difference with enzyme DmtA from Mycobacterium tuberculosis strain H37Rv Mycobacterium tuberculosis variant bovis
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs. R293 is the single amino acid difference with enzyme DmbA from Mycobacterium bovis strain 5033/66 Mycobacterium tuberculosis
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup III of HLDs Mycobacterium tuberculosis variant bovis
evolution the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup III of HLDs Rhodopirellula baltica
additional information the enzyme shows higher activity toward beta-HCH than LinBUT from Sphingobium japonicum strain UT26 Sphingobium indicum
physiological function the enzyme is involved in 1,2-dichloroethane degradation Xanthobacter autotrophicus
physiological function the enzyme is involved in 1,2-dichloroethane degradation Bradyrhizobium elkanii
physiological function the enzyme is involved in 1-haloalkanes degradation Rhodococcus rhodochrous
physiological function the enzyme is involved in gamma-HCH degradation Sphingobium indicum