Cloned (Comment) | Organism |
---|---|
phylogenetic analysis and tree | Corynebacterium sp. |
phylogenetic analysis and tree | Arthrobacter sp. |
phylogenetic analysis and tree | Rhodococcus sp. |
phylogenetic analysis and tree | Strongylocentrotus purpuratus |
phylogenetic analysis and tree | Sphingomonas sp. |
phylogenetic analysis and tree | Mycobacterium tuberculosis variant bovis |
phylogenetic analysis and tree | Sphingobium indicum |
phylogenetic analysis and tree | Xanthobacter autotrophicus |
phylogenetic analysis and tree | Bradyrhizobium japonicum |
phylogenetic analysis and tree | Mesorhizobium loti |
phylogenetic analysis and tree | Sphingobium sp. |
phylogenetic analysis and tree | Mycobacterium tuberculosis |
phylogenetic analysis and tree | Rhodococcus rhodochrous |
phylogenetic analysis and tree | Agrobacterium tumefaciens |
phylogenetic analysis and tree | Plesiocystis pacifica |
phylogenetic analysis and tree | Alcanivorax dieselolei |
phylogenetic analysis and tree | Mycobacterium sp. |
phylogenetic analysis and tree | Bradyrhizobium elkanii |
phylogenetic analysis and tree | Mycobacterium avium |
phylogenetic analysis and tree | Rhodopirellula baltica |
phylogenetic analysis and tree | Marinobacter sp. |
phylogenetic analysis and tree | Psychrobacter cryohalolentis |
phylogenetic analysis and tree | Rhodobacteraceae bacterium UDC319 |
Crystallization (Comment) | Organism |
---|---|
crystal structure determination and analysis | Marinobacter sp. |
crystal structure determination and analysis, PDB ID 1CIJ | Xanthobacter autotrophicus |
crystal structure determination and analysis, PDB ID 1CIJ | Rhodobacteraceae bacterium UDC319 |
crystal structure determination and analysis, PDB ID 1CQW | Rhodococcus rhodochrous |
crystal structure determination and analysis, PDB ID 1CV2/2BFN | Sphingobium indicum |
crystal structure determination and analysis, PDB ID 2QVB | Mycobacterium tuberculosis |
crystal structure determination and analysis, PDB ID 2XT0 | Plesiocystis pacifica |
crystal structure determination and analysis, PDB ID 3WI7 for the wild-type and PDB ID 3WIB for the mutant enzyme | Agrobacterium tumefaciens |
crystal structure determination and analysis, PDB ID 4H77 | Sphingobium sp. |
crystal structure determination and analysis, PDB ID 4K2A | Bradyrhizobium elkanii |
crystal structure determination and analysis, PDB IDs 3A2M and 3AFI for te wild-type, and PDB ID 3A2L for the mutant | Bradyrhizobium japonicum |
crystal structure determination and analysis, PDB IDs 4BRZ and 4C6H | Psychrobacter cryohalolentis |
mutant crystal structure determination and analysis, PDB IDs 4FWB and 3SK0 | Mycobacterium sp. |
Protein Variants | Comment | Organism |
---|---|---|
C176Y/Y273F | site-directed mutagenesis, the mutant enzyme is 3.5fold higher activity toward TCP than DhaA wild-type | Mycobacterium sp. |
H139A | site-directed mutagenesis | Bradyrhizobium japonicum |
additional information | construction of deletion mutant DbeADELTACl, mutation in the second halide-binding site | Bradyrhizobium elkanii |
additional information | construction of deletion mutants DbjADELTA and DbjADELTA H139A | Bradyrhizobium japonicum |
additional information | construction of engineered enzyme variants: the more thermostable Dhla5/Dhla8, the more active DhaAM2(C176Y/Y273F), the DhaA31 mutant that is 32fold higher activity toward TCP than DhaA wild-type, te mutant DhaA12 mimicking DbjA active (insertion of ERB), and the DhaA31 mutant that has higher enantioselectivity toward TCP than the wild-type | Mycobacterium sp. |
additional information | construction of LinB variants, that mimick the DmtA active site, shows higher activity toward beta- and delta-HCH than LinBMI. Mutant 4H7D/4H7E/4H7F/4H7H/4H7I/4H7J/4H7K are intermediates between LinBUT and LinBMI | Sphingobium sp. |
additional information | construction of LinBUT variants, 4H7D/4H7E/4H7F/4H7H/4H7I/4H7J/4H7K are intermediates between LinBUT and LinBMI | Sphingobium indicum |
additional information | engineered enzyme variants are constructed for in vivo evolution for activity toward 1-chlorohexane, more activity toward 1,6-dichlorohexane than DhlA wild-type, and increased activity toward 1,2-dibromoethane/1-bromobutane the wild-type | Xanthobacter autotrophicus |
Y109W | site-directed mutagenesis, mutant DatA01 | Agrobacterium tumefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | E2RV69 | gene datA | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | E2RV69 | gene datA | - |
Alcanivorax dieselolei | - |
- |
- |
Alcanivorax dieselolei B-5 | - |
- |
- |
Arthrobacter sp. | - |
gene dhaA | - |
Bradyrhizobium elkanii | E2RV62 | - |
- |
Bradyrhizobium elkanii USDA94 | E2RV62 | - |
- |
Bradyrhizobium japonicum | P59337 | - |
- |
Bradyrhizobium japonicum USDA 110 | P59337 | - |
- |
Corynebacterium sp. | - |
gene dhaA | - |
Marinobacter sp. | A3JB27 | a psychrophilic organism | - |
Mesorhizobium loti | Q98C03 | - |
- |
Mesorhizobium loti MAFF303099 | Q98C03 | - |
- |
Mycobacterium avium | Q93K00 | gene dhmA | - |
Mycobacterium avium N85 | Q93K00 | gene dhmA | - |
Mycobacterium sp. | Q9ZER0 | gene dhaAF | - |
Mycobacterium sp. GP1 | Q9ZER0 | gene dhaAF | - |
Mycobacterium tuberculosis | P9WMR9 | dhaA | - |
Mycobacterium tuberculosis H37Rv | P9WMR9 | dhaA | - |
Mycobacterium tuberculosis variant bovis | A4Q9R7 | gene dmbC | - |
Mycobacterium tuberculosis variant bovis | Q6EUU8 | gene dmbB | - |
Mycobacterium tuberculosis variant bovis | Q9XB14 | gene dhaA | - |
Mycobacterium tuberculosis variant bovis 5033/66 | A4Q9R7 | gene dmbC | - |
Mycobacterium tuberculosis variant bovis 5033/66 | Q6EUU8 | gene dmbB | - |
Mycobacterium tuberculosis variant bovis 5033/66 | Q9XB14 | gene dhaA | - |
Plesiocystis pacifica | A6G7B1 | - |
- |
Psychrobacter cryohalolentis | Q1QBB9 | gene dhmA, a psychrophilic organism | - |
Psychrobacter cryohalolentis K5 | Q1QBB9 | gene dhmA, a psychrophilic organism | - |
Rhodobacteraceae bacterium UDC319 | A0A023I2Y1 | - |
- |
Rhodococcus rhodochrous | P0A3G2 | gene dhaA | - |
Rhodococcus rhodochrous NCIMB 13064 | P0A3G2 | gene dhaA | - |
Rhodococcus sp. | - |
gene dhaA | - |
Rhodopirellula baltica | G3XCP3 | gene drbA | - |
Rhodopirellula baltica SH1 | G3XCP3 | gene drbA | - |
Sphingobium indicum | - |
- |
- |
Sphingobium indicum | P51698 | gene linB | - |
Sphingobium indicum B90A | - |
- |
- |
Sphingobium indicum UT26 | P51698 | gene linB | - |
Sphingobium sp. | A4PEU6 | - |
- |
Sphingobium sp. MI1205 | A4PEU6 | - |
- |
Sphingomonas sp. | - |
- |
- |
Sphingomonas sp. BHC-A | - |
- |
- |
Strongylocentrotus purpuratus | - |
- |
- |
Xanthobacter autotrophicus | P22643 | gene dhlA | - |
Xanthobacter autotrophicus GJ10 | P22643 | gene dhlA | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with chlorinated compounds | Plesiocystis pacifica | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DadB | - |
Alcanivorax dieselolei |
DatA | - |
Agrobacterium tumefaciens |
DbeA | - |
Bradyrhizobium elkanii |
DbjA | - |
Bradyrhizobium japonicum |
DhaA | - |
Corynebacterium sp. |
DhaA | - |
Arthrobacter sp. |
DhaA | - |
Rhodococcus sp. |
DhaA | - |
Mycobacterium tuberculosis variant bovis |
DhaA | - |
Mycobacterium tuberculosis |
DhaA | - |
Rhodococcus rhodochrous |
DhaB | - |
Mycobacterium tuberculosis variant bovis |
DhaC | - |
Mycobacterium tuberculosis variant bovis |
DhAf | - |
Mycobacterium sp. |
DhlA | - |
Xanthobacter autotrophicus |
DhmA | - |
Mycobacterium avium |
DhmA | - |
Psychrobacter cryohalolentis |
DmaA | - |
Marinobacter sp. |
dmbA | - |
Mycobacterium tuberculosis variant bovis |
DmbB | - |
Mycobacterium tuberculosis variant bovis |
DmbC | - |
Mycobacterium tuberculosis variant bovis |
dmlA | - |
Mesorhizobium loti |
DmtA | - |
Mycobacterium tuberculosis |
DmxA | - |
Marinobacter sp. |
DpcA | - |
Psychrobacter cryohalolentis |
DppA | - |
Plesiocystis pacifica |
DrbA | - |
Rhodopirellula baltica |
DspA | - |
Strongylocentrotus purpuratus |
HanR | - |
Rhodobacteraceae bacterium UDC319 |
HLD | - |
Corynebacterium sp. |
HLD | - |
Arthrobacter sp. |
HLD | - |
Rhodococcus sp. |
HLD | - |
Strongylocentrotus purpuratus |
HLD | - |
Sphingomonas sp. |
HLD | - |
Mycobacterium tuberculosis variant bovis |
HLD | - |
Sphingobium indicum |
HLD | - |
Xanthobacter autotrophicus |
HLD | - |
Bradyrhizobium japonicum |
HLD | - |
Mesorhizobium loti |
HLD | - |
Sphingobium sp. |
HLD | - |
Mycobacterium tuberculosis |
HLD | - |
Rhodococcus rhodochrous |
HLD | - |
Agrobacterium tumefaciens |
HLD | - |
Plesiocystis pacifica |
HLD | - |
Alcanivorax dieselolei |
HLD | - |
Mycobacterium sp. |
HLD | - |
Bradyrhizobium elkanii |
HLD | - |
Mycobacterium avium |
HLD | - |
Rhodopirellula baltica |
HLD | - |
Marinobacter sp. |
HLD | - |
Psychrobacter cryohalolentis |
HLD | - |
Rhodobacteraceae bacterium UDC319 |
LinBMI | - |
Sphingomonas sp. |
LinBMI | - |
Sphingobium indicum |
LinBMI | - |
Sphingobium sp. |
LinBUT | - |
Sphingobium indicum |
Rv2579 | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup I of HLDs | Xanthobacter autotrophicus |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup I of HLDs | Plesiocystis pacifica |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup I of HLDs | Mycobacterium tuberculosis variant bovis |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup I of HLDs | Mycobacterium avium |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup I of HLDs | Psychrobacter cryohalolentis |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs | Corynebacterium sp. |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs | Arthrobacter sp. |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs | Rhodococcus sp. |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs | Strongylocentrotus purpuratus |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs | Sphingomonas sp. |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs | Sphingobium indicum |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs | Bradyrhizobium japonicum |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs | Mesorhizobium loti |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs | Sphingobium sp. |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs | Rhodococcus rhodochrous |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs | Agrobacterium tumefaciens |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs | Alcanivorax dieselolei |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs | Mycobacterium sp. |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs | Bradyrhizobium elkanii |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs | Marinobacter sp. |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs | Rhodobacteraceae bacterium UDC319 |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs. Q293 is the single amino acid difference with enzyme DmtA from Mycobacterium tuberculosis strain H37Rv | Mycobacterium tuberculosis variant bovis |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup II of HLDs. R293 is the single amino acid difference with enzyme DmbA from Mycobacterium bovis strain 5033/66 | Mycobacterium tuberculosis |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup III of HLDs | Mycobacterium tuberculosis variant bovis |
evolution | the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup III of HLDs | Rhodopirellula baltica |
additional information | the enzyme shows higher activity toward beta-HCH than LinBUT from Sphingobium japonicum strain UT26 | Sphingobium indicum |
physiological function | the enzyme is involved in 1,2-dichloroethane degradation | Xanthobacter autotrophicus |
physiological function | the enzyme is involved in 1,2-dichloroethane degradation | Bradyrhizobium elkanii |
physiological function | the enzyme is involved in 1-haloalkanes degradation | Rhodococcus rhodochrous |
physiological function | the enzyme is involved in gamma-HCH degradation | Sphingobium indicum |