Cloned (Comment) | Organism |
---|---|
gene dmmA from cosmid pLM17, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of wild-type and mutants in Escherichia coli strain BL21(DE3) | uncultured marine bacterium |
Crystallization (Comment) | Organism |
---|---|
purified recombinant selenomethionyl and bative DmmAshort and native DmmAlong form, vapor diffusion, for the short form: mixing of equal volumes of protein solution containing 6 mg/mL DmmAshort in 20 mM Tris, pH 7.9, 500 mM NaCl, 10% glycerol, with well solution containing 2.2 M sodium malonate, pH 7.0, 5% glycerol, 20°C, 3-4 days, for the long form: mixing of equal volumes of protein solution containing 14 mg/mL DmmAlong in 20 mM Tris, pH 7.9, 500 mM NaCl, 10% glycerol, with well solution containing 0.6 M lithium sulfate, 27.5% PEG3350, and 0.1 M BisTris, pH 5.5, cryoprotection by 25% PEG400, X-ray diffraction structure determination and analysis at 2.2 A and 2.9 A resolution, respectively | uncultured marine bacterium |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a truncated enzyme form lacking the N-terminus, DmmAshort, from full-length wild-type enzyme, DmmAlong | uncultured marine bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, overview | uncultured marine bacterium | |
0.24 | - |
1,3-dibromopropane | pH and temperature not specified in the publication | uncultured marine bacterium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-dibromopropane + H2O | uncultured marine bacterium | - |
3-bromopropanol + bromide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
no activity in Moorea producta | - |
formerly Lyngbya majuscula | - |
uncultured marine bacterium | - |
gene dmmA, formerly curN, from cosmid pLM17 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | uncultured marine bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-dibromopropane + H2O | - |
uncultured marine bacterium | 3-bromopropanol + bromide | - |
? | |
1,3-dibromopropane + H2O | preferred substrate | uncultured marine bacterium | 3-bromopropanol + bromide | - |
? | |
1,6-dibromohexane + H2O | low activity | uncultured marine bacterium | 6-bromohexanol + bromide | - |
? | |
1,6-dichlorohexane + H2O | low activity | uncultured marine bacterium | 6-chlorohexanol + chloride | - |
? | |
1,6-diiodohexane + H2O | low activity | uncultured marine bacterium | 6-iodohexane + iodide | - |
? | |
bromocyclohexane + H2O | - |
uncultured marine bacterium | cyclohexanol + bromide | - |
? | |
additional information | substrate specificity, overview | uncultured marine bacterium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer or dimer | dynamic equilibrium between monomeric and dimeric forms | uncultured marine bacterium |
More | DmmA adopts the alpha/beta hydrolase fold, consisting of core and lid sub-domains with the active site at the top of the core, which is covered by the lid, quaternary structure comparisons, overview | uncultured marine bacterium |
Synonyms | Comment | Organism |
---|---|---|
DmmA | formerly CurN | uncultured marine bacterium |
haloalkane dehalogenase | - |
uncultured marine bacterium |
HLD | - |
uncultured marine bacterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
1,3-dibromopropane | pH and temperature not specified in the publication | uncultured marine bacterium |
General Information | Comment | Organism |
---|---|---|
evolution | DmmA belongs to the haloalkane dehalogenase family, subfamily-II, structure comparisons, overview | uncultured marine bacterium |
additional information | the active site is formed by a pentad consisting of halide-stabilizing Asn78 and Trp145, nucleophile Asp144, base His315, and acid Glu168. DmmA possesses a larg active site binding pocket allowing to accept a range of linear and cyclic substrates including include chlorinated, brominated, and iodinated alkanes of varying length, structure, overview | uncultured marine bacterium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
1,3-dibromopropane | pH and temperature not specified in the publication | uncultured marine bacterium |