Literature summary for 3.8.1.5 extracted from

Gehret, J.J.; Gu, L.; Geders, T.W.; Brown, W.C.; Gerwick, L.; Gerwick, W.H.; Sherman, D.H.; Smith, J.L.
Structure and activity of DmmA, a marine haloalkane dehalogenase (2012), Protein Sci., 21, 239-248.

Cloned(Commentary)

Cloned (Comment)	Organism
gene dmmA from cosmid pLM17, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of wild-type and mutants in Escherichia coli strain BL21(DE3)	uncultured marine bacterium

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant selenomethionyl and bative DmmAshort and native DmmAlong form, vapor diffusion, for the short form: mixing of equal volumes of protein solution containing 6 mg/mL DmmAshort in 20 mM Tris, pH 7.9, 500 mM NaCl, 10% glycerol, with well solution containing 2.2 M sodium malonate, pH 7.0, 5% glycerol, 20°C, 3-4 days, for the long form: mixing of equal volumes of protein solution containing 14 mg/mL DmmAlong in 20 mM Tris, pH 7.9, 500 mM NaCl, 10% glycerol, with well solution containing 0.6 M lithium sulfate, 27.5% PEG3350, and 0.1 M BisTris, pH 5.5, cryoprotection by 25% PEG400, X-ray diffraction structure determination and analysis at 2.2 A and 2.9 A resolution, respectively	uncultured marine bacterium

Crystallization (Comment)

Organism

purified recombinant selenomethionyl and bative DmmAshort and native DmmAlong form, vapor diffusion, for the short form: mixing of equal volumes of protein solution containing 6 mg/mL DmmAshort in 20 mM Tris, pH 7.9, 500 mM NaCl, 10% glycerol, with well solution containing 2.2 M sodium malonate, pH 7.0, 5% glycerol, 20°C, 3-4 days, for the long form: mixing of equal volumes of protein solution containing 14 mg/mL DmmAlong in 20 mM Tris, pH 7.9, 500 mM NaCl, 10% glycerol, with well solution containing 0.6 M lithium sulfate, 27.5% PEG3350, and 0.1 M BisTris, pH 5.5, cryoprotection by 25% PEG400, X-ray diffraction structure determination and analysis at 2.2 A and 2.9 A resolution, respectively

uncultured marine bacterium

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of a truncated enzyme form lacking the N-terminus, DmmAshort, from full-length wild-type enzyme, DmmAlong	uncultured marine bacterium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	steady-state kinetics, overview	uncultured marine bacterium
0.24	-	1,3-dibromopropane	pH and temperature not specified in the publication	uncultured marine bacterium

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1,3-dibromopropane + H2O	uncultured marine bacterium	-	3-bromopropanol + bromide	-	?

Organism

Organism	UniProt	Comment	Textmining
no activity in Moorea producta	-	formerly Lyngbya majuscula	-
uncultured marine bacterium	-	gene dmmA, formerly curN, from cosmid pLM17	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration	uncultured marine bacterium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
1,3-dibromopropane + H2O	-	uncultured marine bacterium	3-bromopropanol + bromide	-	?
1,3-dibromopropane + H2O	preferred substrate	uncultured marine bacterium	3-bromopropanol + bromide	-	?
1,6-dibromohexane + H2O	low activity	uncultured marine bacterium	6-bromohexanol + bromide	-	?
1,6-dichlorohexane + H2O	low activity	uncultured marine bacterium	6-chlorohexanol + chloride	-	?
1,6-diiodohexane + H2O	low activity	uncultured marine bacterium	6-iodohexane + iodide	-	?
bromocyclohexane + H2O	-	uncultured marine bacterium	cyclohexanol + bromide	-	?
additional information	substrate specificity, overview	uncultured marine bacterium	?	-	?

Subunits

Subunits	Comment	Organism
monomer or dimer	dynamic equilibrium between monomeric and dimeric forms	uncultured marine bacterium
More	DmmA adopts the alpha/beta hydrolase fold, consisting of core and lid sub-domains with the active site at the top of the core, which is covered by the lid, quaternary structure comparisons, overview	uncultured marine bacterium

Synonyms

Synonyms	Comment	Organism
DmmA	formerly CurN	uncultured marine bacterium
haloalkane dehalogenase	-	uncultured marine bacterium
HLD	-	uncultured marine bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.4	-	1,3-dibromopropane	pH and temperature not specified in the publication	uncultured marine bacterium

General Information

General Information	Comment	Organism
evolution	DmmA belongs to the haloalkane dehalogenase family, subfamily-II, structure comparisons, overview	uncultured marine bacterium
additional information	the active site is formed by a pentad consisting of halide-stabilizing Asn78 and Trp145, nucleophile Asp144, base His315, and acid Glu168. DmmA possesses a larg active site binding pocket allowing to accept a range of linear and cyclic substrates including include chlorinated, brominated, and iodinated alkanes of varying length, structure, overview	uncultured marine bacterium

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
10	-	1,3-dibromopropane	pH and temperature not specified in the publication	uncultured marine bacterium