Protein Variants | Comment | Organism |
---|---|---|
L177W | naturally occuring mutation, the single mutation in a tunnel to the active site changes the mechanism and kinetics of product release in LinB. Interactions of the bromide ion with the tryptophan increase free energy barrier for its passage, causing the reaction mechanism change | Sphingobium indicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state and transient kinetics, kinetics of bromide ion binding, overview. Two distinct binding phases that suggest a two-step mechanism of bromide ion binding | Sphingobium indicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium indicum | - |
- |
- |
Sphingobium indicum UT26 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
1-haloalkane + H2O = a primary alcohol + halide | reaction mechanism, overview. The bromide release is the rate-limiting step of the catalytic cycle, overview | Sphingobium indicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-haloalkane + H2O | - |
Sphingobium indicum | a primary alcohol + halide | - |
? | |
1-haloalkane + H2O | - |
Sphingobium indicum UT26 | a primary alcohol + halide | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structural modeling using crystal structure, PDB code 1MJ5, overview | Sphingobium indicum |
Synonyms | Comment | Organism |
---|---|---|
LinB | - |
Sphingobium indicum |
General Information | Comment | Organism |
---|---|---|
malfunction | the single mutation in a tunnel to the active site changes the mechanism and kinetics of product release in LinB. Interactions of the bromide ion with the tryptophan increase free energy barrier for its passage, causing the reaction mechanism change | Sphingobium indicum |