Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.8.1.5 extracted from

  • Chaloupkova, R.; Prokop, Z.; Sato, Y.; Nagata, Y.; Damborsky, J.
    Stereoselectivity and conformational stability of haloalkane dehalogenase DbjA from Bradyrhizobium japonicum USDA110: the effect of pH and temperature (2011), FEBS J., 278, 2728-2738.
    View publication on PubMed
Search for more literature for 3.8.1.5

Application

Application Comment Organism
synthesis unique catalytic activity and structural stability of DbjA in a broad pH range, combined with high enantioselectivity with particular substrates, make this enzyme a very versatile biocatalyst Bradyrhizobium japonicum

Organism

Organism UniProt Comment Textmining
Bradyrhizobium japonicum P59337
-
-
Bradyrhizobium japonicum USDA 110 P59337
-
-

Reaction

Reaction Comment Organism Reaction ID
1-haloalkane + H2O = a primary alcohol + halide Asp103, Glu127 and His280 are involved in the catalytic reaction, and two H-bond donating residues, Asn38 and Trp104, are involved in stabilization of a halogen group of the substrate Bradyrhizobium japonicum
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-iodohexane + H2O
-
 Bradyrhizobium japonicum 1-hexanol + iodide
-
 ?
1-iodohexane + H2O
-
 Bradyrhizobium japonicum USDA 110 1-hexanol + iodide
-
 ?
2-bromobutane + H2O
-
 Bradyrhizobium japonicum 2-butanol + bromide
-
 ?
2-bromobutane + H2O
-
 Bradyrhizobium japonicum USDA 110 2-butanol + bromide
-
 ?
2-bromopentane + H2O
-
 Bradyrhizobium japonicum 2-pentanol + bromide
-
 ?
2-bromopentane + H2O
-
 Bradyrhizobium japonicum USDA 110 2-pentanol + bromide
-
 ?
ethyl 2-bromobutyrate + H2O
-
 Bradyrhizobium japonicum ?
-
 ?
ethyl 2-bromobutyrate + H2O
-
 Bradyrhizobium japonicum USDA 110 ?
-
 ?
methyl 2-bromopropionate + H2O
-
 Bradyrhizobium japonicum ?
-
 ?
additional information the pH does not have any effect on enantiodiscriminination of DbjA, but a decrease in temperature results in significantly enhanced enantioselectivity Bradyrhizobium japonicum ?
-
 ?
additional information the pH does not have any effect on enantiodiscriminination of DbjA, but a decrease in temperature results in significantly enhanced enantioselectivity Bradyrhizobium japonicum USDA 110 ?
-
 ?

Subunits

Subunits Comment Organism
dimer at pH 6.1-6.5, SDS-PAGE and gel filtration Bradyrhizobium japonicum
monomer at pH 5.7-5.9, SDS-PAGE and gel filtration Bradyrhizobium japonicum
More oligomerization of DbjA is strongly pH-dependent: monomer, dimer, tetramer and a high molecular weight cluster of the enzyme occur in solution at different pH conditions, overview Bradyrhizobium japonicum
multimer in solution above pH 9.6 Bradyrhizobium japonicum
tetramer
-
 Bradyrhizobium japonicum

Synonyms

Synonyms Comment Organism
DbjA
-
 Bradyrhizobium japonicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Bradyrhizobium japonicum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 50 highest activity Bradyrhizobium japonicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 different oligomeric states of DbjA possess different thermal stabilities. The enzyme is most stable at 49.1°C and pH 6.5 as a dimer Bradyrhizobium japonicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7 10.4
-
 Bradyrhizobium japonicum

pH Range

pH Minimum pH Maximum Comment Organism
7 11 DbjA activity decreases below pH 7.0 and above pH 10.4 with no activity detected below pH 5.0 and above pH 11.0 Bradyrhizobium japonicum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5
-
 different oligomeric states of DbjA possess different thermal stabilities. The enzyme is most stable at 49.1°C and pH 6.5 as a dimer Bradyrhizobium japonicum

pI Value

Organism Comment pI Value Maximum pI Value
Bradyrhizobium japonicum sequence calculation
-
 5.89