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Literature summary for 3.8.1.5 extracted from

  • Hesseler, M.; Bogdanovic, X.; Hidalgo, A.; Berenguer, J.; Palm, G.J.; Hinrichs, W.; Bornscheuer, U.T.
    Cloning, functional expression, biochemical characterization, and structural analysis of a haloalkane dehalogenase from Plesiocystis pacifica SIR-1 (2011), Appl. Microbiol. Biotechnol., 91, 1049-1060.
    View publication on PubMed
Search for more literature for 3.8.1.5

Cloned(Commentary)

Cloned (Comment) Organism
gene dppA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21-Gold(DE3) Plesiocystis pacifica

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, hanging-drop vapor-diffusion method, 8 mg/ml protein in 10 mM Tris-HCl buffer, pH 8.0, is mixed with a reservoir solution containing 0.1 M Na-MES, pH 6.5, 1.8 M (NH4)2SO4, and 5% PEG 400, 20°C, X-ray structure determination and analysis at 1.95 A resolution Plesiocystis pacifica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.75
-
 1-bromoheptane pH 8.2, 35°C Plesiocystis pacifica
1.57
-
 1,2-Dibromoethane pH 8.2, 35°C Plesiocystis pacifica
1.7
-
 1-bromohexane pH 8.2, 35°C Plesiocystis pacifica
1.96
-
 1,2-dibromopropane pH 8.2, 35°C Plesiocystis pacifica
2.51
-
 1-bromopentane pH 8.2, 35°C Plesiocystis pacifica
24
-
 1-bromobutane pH 8.2, 35°C Plesiocystis pacifica
45.2
-
 1-Bromopropane pH 8.2, 35°C Plesiocystis pacifica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32600
-
 1 * 32600, SDS-PAGE Plesiocystis pacifica

Organism

Organism UniProt Comment Textmining
Plesiocystis pacifica A6G7B1 DSM14875, gene dppA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Plesiocystis pacifica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dibromoethane + H2O
-
 Plesiocystis pacifica 2-bromo-1-ethanol + bromide
-
 ?
1,2-dibromopropane + H2O
-
 Plesiocystis pacifica 2-bromo-1-propanol + bromide
-
 ?
1-bromobutane + H2O preferred substrate Plesiocystis pacifica 1-butanol + bromide
-
 ?
1-bromoheptane + H2O
-
 Plesiocystis pacifica 1-heptanol + bromide
-
 ?
1-bromohexane + H2O
-
 Plesiocystis pacifica 1-hexanol + bromide
-
 ?
1-bromononane + H2O
-
 Plesiocystis pacifica 1-nonanol + bromide
-
 ?
1-bromooctane + H2O
-
 Plesiocystis pacifica 1-octanol + bromide
-
 ?
1-bromopentane + H2O
-
 Plesiocystis pacifica 1-pentanol + bromide
-
 ?
1-bromopropane + H2O
-
 Plesiocystis pacifica 1-propanol + bromide
-
 ?
2-bromobutane + H2O
-
 Plesiocystis pacifica 2-butanol + bromide
-
 ?
additional information DppA shows high preference for 1-bromobutane and does not accept chlorinated alkanes, haloacids, or haloalcohols, substrate specificity, overview Plesiocystis pacifica ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 32600, SDS-PAGE Plesiocystis pacifica
More the enzyme is composed of an alpha/beta-hydrolase fold and a cap domain and the overall fold is similar to other known haloalkane dehalogenases. DppA possesses a large and open active site pocket, docking, overview Plesiocystis pacifica

Synonyms

Synonyms Comment Organism
DppA
-
 Plesiocystis pacifica
HLD
-
 Plesiocystis pacifica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
33 37
-
 Plesiocystis pacifica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.38
-
 1,2-Dibromoethane pH 8.2, 35°C Plesiocystis pacifica
1.39
-
 1-bromoheptane pH 8.2, 35°C Plesiocystis pacifica
1.97
-
 1,2-dibromopropane pH 8.2, 35°C Plesiocystis pacifica
2.69
-
 1-bromohexane pH 8.2, 35°C Plesiocystis pacifica
5.85
-
 1-Bromopropane pH 8.2, 35°C Plesiocystis pacifica
8.08
-
 1-bromobutane pH 8.2, 35°C Plesiocystis pacifica
9.94
-
 1-bromopentane pH 8.2, 35°C Plesiocystis pacifica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
 Plesiocystis pacifica

General Information

General Information Comment Organism
evolution DppA is a member of the haloalkane dehalogenase subfamily HLD-I Plesiocystis pacifica
additional information active site residues are Asp123, His278, and Asp249, and Trp124 and Trp163 as halide-stabilizing residues, and a catalytic triad Asp-His-Asp Plesiocystis pacifica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.13
-
 1-Bromopropane pH 8.2, 35°C Plesiocystis pacifica
0.24
-
 1,2-Dibromoethane pH 8.2, 35°C Plesiocystis pacifica
0.34
-
 1-bromobutane pH 8.2, 35°C Plesiocystis pacifica
1
-
 1,2-dibromopropane pH 8.2, 35°C Plesiocystis pacifica
1.58
-
 1-bromohexane pH 8.2, 35°C Plesiocystis pacifica
1.84
-
 1-bromoheptane pH 8.2, 35°C Plesiocystis pacifica
3.96
-
 1-bromopentane pH 8.2, 35°C Plesiocystis pacifica