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Literature summary for 3.8.1.5 extracted from

  • Schindler, J.F.; Naranjo, P.A.; Honaberger, D.A.; Chang, C.H.; Brainard, J.R.; Vanderberg, L.A.; Unkefer, C.J.
    Haloalkane dehalogenases: steady-state kinetics and halide inhibition (1999), Biochemistry, 38, 5772-5778.
    View publication on PubMed
Search for more literature for 3.8.1.5

Protein Variants

Protein Variants Comment Organism
W175F mutant with no inhibition through halide product Xanthobacter autotrophicus
W175Y mutant with no inhibition through halide product Xanthobacter autotrophicus

Inhibitors

Inhibitors Comment Organism Structure
halide product shows uncompetitive, pH-dependent inhibition pattern, maximal inhibition at optimal pH Xanthobacter autotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
secondary haloalkanes + H2O Rhodococcus erythropolis
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis
-
-
-
Xanthobacter autotrophicus
-
 GJ10
-
Xanthobacter autotrophicus GJ10
-
 GJ10
-

Reaction

Reaction Comment Organism Reaction ID
1-haloalkane + H2O = a primary alcohol + halide GJ10, four-step reaction mechanism with covalent ester intermediate Xanthobacter autotrophicus
a
1-haloalkane + H2O = a primary alcohol + halide alpha/beta hydrolase with catalytic triad, i.e. nucleophile (Asp124)-histidine (H289)-acid (Asp260), during catalytic reaction Xanthobacter autotrophicus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dichloroethane + H2O
-
 Xanthobacter autotrophicus 2-chloroethanol + chloride
-
 ?
1,2-dichloroethane + H2O
-
 Xanthobacter autotrophicus GJ10 2-chloroethanol + chloride
-
 ?
1,3-dibromo-2-propanol + H2O
-
 Rhodococcus erythropolis 3-bromo-1,2-propandiol
-
 ?
1,3-dibromo-2-propanol + H2O
-
 Xanthobacter autotrophicus 3-bromo-1,2-propandiol
-
 ?
1,3-dibromo-2-propanol + H2O
-
 Xanthobacter autotrophicus GJ10 3-bromo-1,2-propandiol
-
 ?
1-chlorobutane + H2O
-
 Rhodococcus erythropolis butanol + chloride
-
 ?
1-chlorobutane + H2O
-
 Xanthobacter autotrophicus butanol + chloride
-
 ?
1-chlorobutane + H2O
-
 Xanthobacter autotrophicus GJ10 butanol + chloride
-
 ?
2-bromoethanol + H2O
-
 Rhodococcus erythropolis ethanol + bromide
-
 ?
2-bromoethanol + H2O
-
 Xanthobacter autotrophicus ethanol + bromide
-
 ?
2-bromoethanol + H2O
-
 Xanthobacter autotrophicus GJ10 ethanol + bromide
-
 ?
haloalkane + H2O
-
 Rhodococcus erythropolis alcohol + halide
-
 ?
haloalkane + H2O
-
 Xanthobacter autotrophicus alcohol + halide
-
 ?
haloalkane + H2O
-
 Xanthobacter autotrophicus GJ10 alcohol + halide
-
 ?
secondary haloalkanes + H2O
-
 Rhodococcus erythropolis ?
-
 ?