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Literature summary for 3.8.1.5 extracted from

  • Nagata, Y.; Miyauchi, K.; Damborsky, J.; Manova, K.; Ansorgova, A.; Takagi, M.
    Purification and characterization of a haloalkane dehalogenase of a new substrate class from a gamma-hexachlorocyclohexane-degrading bacterium, Sphingomonas paucimobilis UT26 (1997), Appl. Environ. Microbiol., 63, 3707-3710.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.8.1.5

Application

Application Comment Organism
agriculture biodegradation of pollutant insecticide gamma-hexachlorocyclohexane (lindane) Sphingomonas paucimobilis

Cloned(Commentary)

Cloned (Comment) Organism
UT26, overexpression in Escherichia coli Sphingomonas paucimobilis

Protein Variants

Protein Variants Comment Organism
additional information UT26, W175 probably naturally exchanged for F or Q explaining substrate specificities Sphingomonas paucimobilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 gel filtration, SDS-PAGE Sphingomonas paucimobilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,3,4,6-tetrachloro-1,4,cyclohexadiene + 2 H2O Sphingomonas paucimobilis
-
 2,5-dichloro-2,5-cyclohexadiene-1,4-diol + 2 chloride
-
 ?
n-haloalkanes + H2O Sphingomonas paucimobilis broad substrate range primary alcohols + halide
-
 ?

Organism

Organism UniProt Comment Textmining
Sphingomonas paucimobilis
-
 e.g. Pseudomonas paucimobilis
-
Sphingomonas paucimobilis
-
 UT26
-
Sphingomonas paucimobilis UT26
-
 UT26
-

Purification (Commentary)

Purification (Comment) Organism
UT26 Sphingomonas paucimobilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dibromoethane + H2O
-
 Sphingomonas paucimobilis 2-bromoethanol + bromide
-
 ?
1,2-dibromoethane + H2O
-
 Sphingomonas paucimobilis UT26 2-bromoethanol + bromide
-
 ?
1,2-dichloroethane + H2O
-
 Sphingomonas paucimobilis 2-chloroethanol + chloride
-
 ?
1,2-dichloroethane + H2O
-
 Sphingomonas paucimobilis UT26 2-chloroethanol + chloride
-
 ?
1,3,4,6-tetrachloro-1,4,cyclohexadiene + 2 H2O
-
 Sphingomonas paucimobilis 2,5-dichloro-2,5-cyclohexadiene-1,4-diol + 2 chloride
-
 ?
1-chloro-2-bromoethane + H2O
-
 Sphingomonas paucimobilis ?
-
 ?
1-chlorobutane + H2O
-
 Sphingomonas paucimobilis butanol + chloride
-
 ?
1-chlorobutane + H2O
-
 Sphingomonas paucimobilis UT26 butanol + chloride
-
 ?
haloalkane + H2O broad specificity Sphingomonas paucimobilis alcohol + halide
-
 ?
haloalkane + H2O alpha,omega-dihalogenated ethanes, preference for long-chain haloalkanes Sphingomonas paucimobilis alcohol + halide
-
 ?
haloalkane + H2O broad specificity Sphingomonas paucimobilis UT26 alcohol + halide
-
 ?
haloalkane + H2O alpha,omega-dihalogenated ethanes, preference for long-chain haloalkanes Sphingomonas paucimobilis UT26 alcohol + halide
-
 ?
n-haloalkanes + H2O broad substrate range Sphingomonas paucimobilis primary alcohols + halide
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 32000, SDS-PAGE Sphingomonas paucimobilis

Synonyms

Synonyms Comment Organism
1,3,4,6,-tetrachloro-1,4-cyclohexadiene halidohydrolase
-
 Sphingomonas paucimobilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
 Sphingomonas paucimobilis