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Literature summary for 3.8.1.2 extracted from

  • Kumar, A.; Pillay, B.; Olaniran, A.O.
    L-2-Haloacid dehalogenase from Ancylobacter aquaticus UV5 Sequence determination and structure prediction (2016), Int. J. Biol. Macromol., 83, 216-225 .
    View publication on PubMed
Search for more literature for 3.8.1.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
 Monochloroacetate pH 9.0, 30°C Ancylobacter aquaticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24238
-
 calculated from sequence Ancylobacter aquaticus
25000
-
 SDS-PAGE Ancylobacter aquaticus

Organism

Organism UniProt Comment Textmining
Ancylobacter aquaticus
-
-
-
Ancylobacter aquaticus UV5
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ancylobacter aquaticus

Storage Stability

Storage Stability Organism
-20°C, 50 mM Tris-PO4 buffer (pH 9.0), purified protein retains about 90% activity after storage for 3 months Ancylobacter aquaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2-dichloropropionate + H2O 53% of the activity compared to monochloroacetate Ancylobacter aquaticus propionate + HCl
-
 ?
2,2-dichloropropionate + H2O 53% of the activity compared to monochloroacetate Ancylobacter aquaticus UV5 propionate + HCl
-
 ?
L-2-monochloropropionate + H2O activity with D-2-monochloropropionate is 5% compared to activity with L-2-monochloropropionate Ancylobacter aquaticus D-2-hydroxypropionate + HCl
-
 ?
L-2-monochloropropionate + H2O activity with D-2-monochloropropionate is 5% compared to activity with L-2-monochloropropionate Ancylobacter aquaticus UV5 D-2-hydroxypropionate + HCl
-
 ?
monobromoacetate + H2O 86% of the activity compared to monochloroacetate Ancylobacter aquaticus glycolate + HBr
-
 ?
monobromoacetate + H2O 86% of the activity compared to monochloroacetate Ancylobacter aquaticus UV5 glycolate + HBr
-
 ?
monochloroacetate + H2O
-
 Ancylobacter aquaticus glycolate + HCl
-
 ?
monochloroacetate + H2O
-
 Ancylobacter aquaticus UV5 glycolate + HCl
-
 ?
monoiodoacetate + H2O 73% of the activity compared to monochloroacetate Ancylobacter aquaticus glycolate + HI
-
 ?
monoiodoacetate + H2O 73% of the activity compared to monochloroacetate Ancylobacter aquaticus UV5 glycolate + HI
-
 ?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE Ancylobacter aquaticus

Synonyms

Synonyms Comment Organism
L-2-DhlB
-
 Ancylobacter aquaticus
L-2-haloacid dehalogenase
-
 Ancylobacter aquaticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Ancylobacter aquaticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 25°C: about 45% of maximal activity, 45°C: about 45% of maximal activity Ancylobacter aquaticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, 50% loss of activity Ancylobacter aquaticus
60
-
 rapid inactivation Ancylobacter aquaticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
 Monochloroacetate pH 9.0, 30°C Ancylobacter aquaticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Ancylobacter aquaticus

pH Range

pH Minimum pH Maximum Comment Organism
5 11 pH 5.0: about 55% of maximal activity, pH 11.0: about 50% of maximal activity Ancylobacter aquaticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 10 the enzyme is stable between pH 7-10 retaining 100% of its activity Ancylobacter aquaticus

pI Value

Organism Comment pI Value Maximum pI Value
Ancylobacter aquaticus calculated from sequence
-
 5.18