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Literature summary for 3.8.1.2 extracted from

  • Liao, L.; Sun, X.; Zeng, Y.; Luo, W.; Yu, Y.; Chen, B.
    A new L-haloacid dehalogenase from the Arctic psychrotrophic Pseudoalteromonas sp. BSW20308 (2015), Polar Biol., 38, 1161-1169.
No PubMed abstract available
Search for more literature for 3.8.1.2

Application

Application Comment Organism
additional information the L-haloacid dehalogenase from a psychrotrophic Pseudoalteromonas from the Arctic revealed its potential in industrial applications such as in synthetic chemistry and environmental protection Pseudoalteromonas sp.

Cloned(Commentary)

Cloned (Comment) Organism
phylogenetic analysis, recombinant expression of N-terminally His- and S-tagged enzyme in Escherichia coli strain BMRosetta Pseudoalteromonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 monobromoacetic acid pH 8.2, 25°C Pseudoalteromonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27800
-
 x * 27800, about, sequence calculation, x * 25600-35600, recombinant tagged enzyme, SDS-PAGE, x * 32500, about, recombinant tagged enzyme, sequence calculation Pseudoalteromonas sp.
32500
-
 x * 27800, about, sequence calculation, x * 25600-35600, recombinant tagged enzyme, SDS-PAGE, x * 32500, about, recombinant tagged enzyme, sequence calculation Pseudoalteromonas sp.

Organic Solvent Stability

Organic Solvent Comment Organism
acetonitrile 20-40% of activity remains after incubation in 40% of DMSO Pseudoalteromonas sp.
DMSO about 92% of activity remains after incubation in 40% of DMSO Pseudoalteromonas sp.
Ethanol 20-40% of activity remains after incubation in 40% of DMSO Pseudoalteromonas sp.
Methanol 20-40% of activity remains after incubation in 40% of DMSO Pseudoalteromonas sp.

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas sp.
-
 isolated from the Arctic marine environment
-
Pseudoalteromonas sp. BSW20308
-
 isolated from the Arctic marine environment
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His- and S-tagged enzyme from Escherichia coli strain BMRosetta by affinity purification followed by gel filtration, to homogeneity Pseudoalteromonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-bromopropionic acid + H2O 41% activity compared to monobromoacetic acid Pseudoalteromonas sp. (R)-2-hydroxypropionic acid + bromide
-
 ?
(S)-2-bromopropionic acid + H2O 41% activity compared to monobromoacetic acid Pseudoalteromonas sp. BSW20308 (R)-2-hydroxypropionic acid + bromide
-
 ?
(S)-2-chlorobutyric acid + H2O below 10% activity compared to monobromoacetic acid Pseudoalteromonas sp. (R)-2-hydroxybutyric acid + chloride
-
 ?
(S)-2-chlorobutyric acid + H2O below 10% activity compared to monobromoacetic acid Pseudoalteromonas sp. BSW20308 (R)-2-hydroxybutyric acid + chloride
-
 ?
(S)-2-chloropropionic acid + H2O 14% activity compared to monobromoacetic acid Pseudoalteromonas sp. (R)-2-hydroxypropionic acid + chloride
-
 ?
(S)-2-chloropropionic acid + H2O 14% activity compared to monobromoacetic acid Pseudoalteromonas sp. BSW20308 (R)-2-hydroxypropionic acid + chloride
-
 ?
dichloroacetic acid + H2O below 10% activity compared to monobromoacetic acid Pseudoalteromonas sp. ?
-
 ?
dichloroacetic acid + H2O below 10% activity compared to monobromoacetic acid Pseudoalteromonas sp. BSW20308 ?
-
 ?
monobromoacetic acid + H2O best substrate Pseudoalteromonas sp. acetic acid + bromide
-
 ?
monobromoacetic acid + H2O best substrate Pseudoalteromonas sp. BSW20308 acetic acid + bromide
-
 ?

Subunits

Subunits Comment Organism
? x * 27800, about, sequence calculation, x * 25600-35600, recombinant tagged enzyme, SDS-PAGE, x * 32500, about, recombinant tagged enzyme, sequence calculation Pseudoalteromonas sp.

Synonyms

Synonyms Comment Organism
2-HAD
-
 Pseudoalteromonas sp.
2-haloacid dehalogenase
-
 Pseudoalteromonas sp.
2-haloalkanoate dehalogenase
-
 Pseudoalteromonas sp.
HADIIBSW
-
 Pseudoalteromonas sp.
L-HAD
-
 Pseudoalteromonas sp.
L-haloacid dehalogenase
-
 Pseudoalteromonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pseudoalteromonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 65 activity range, 20% of maximal activity at 10°C, about 80% at 60°C, inactivation above 65°C Pseudoalteromonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 purified recombinant enzyme, 2 h, completely stable up to Pseudoalteromonas sp.
60
-
 purified recombinant enzyme, 2 h, 40% activity remaining Pseudoalteromonas sp.
65
-
 purified recombinant enzyme, 2 h, inactivation Pseudoalteromonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
 assay at Pseudoalteromonas sp.

General Information

General Information Comment Organism
evolution 2-HADs can be classified into two phylogenetically distinct groups according to their substrate specificity. Group II consists of only L-HADs, which selectively act on L-2-haloalkanoic acids to produce D-2-hydroxyacids. HADIIBSW is evolutionarily distinct from previously characterized L-HADs, sharing only 34% identity with L-HAD (DehRhb) from a Rhodobacteraceae family bacterium UDC319 as the closest relative Pseudoalteromonas sp.