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Literature summary for 3.8.1.2 extracted from

  • Kurihara, T.
    A mechanistic analysis of enzymatic degradation of organohalogen compounds (2011), Biosci. Biotechnol. Biochem., 75, 189-198.
    View publication on PubMed
Search for more literature for 3.8.1.2

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant S175A in complex with chloroacetate, 2-L-chlorobutyrate, 2-L-chloro-3-methylbutyrate, 2-L-chloro-4-methylvalerate, and L-2-chloropropionamide, all substrates bound have D-configuration, except for chloroacetate Pseudomonas sp.

Protein Variants

Protein Variants Comment Organism
D180A site-directed mutagenesis of a residue that strongly interacts with the substrate Pseudomonas sp.
K151A site-directed mutagenesis of a residue that strongly interacts with the substrate Pseudomonas sp.
S175A site-directed mutagenesis of a residue that strongly interacts with the substrate Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-2-chloropropionic acid + H2O Pseudomonas sp.
-
 (R)-2-hydroxypropionic acid + chloride
-
 ?
(S)-2-chloropropionic acid + H2O Pseudomonas sp. 113
-
 (R)-2-hydroxypropionic acid + chloride
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Reaction

Reaction Comment Organism Reaction ID
(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide reaction mechanism, overview Pseudomonas sp.
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-chloropropionic acid + H2O
-
 Pseudomonas sp. (R)-2-hydroxypropionic acid + chloride
-
 ?
(S)-2-chloropropionic acid + H2O the substrate interacts strongly with Asp10, Arg41, Lys151, and Asp180 Pseudomonas sp. (R)-2-hydroxypropionic acid + chloride
-
 ?
(S)-2-chloropropionic acid + H2O
-
 Pseudomonas sp. 113 (R)-2-hydroxypropionic acid + chloride
-
 ?
additional information the enzyme reaction mechanism proceeds via an ester intermediate and a nucleophilic aspartate attacking the C-atom of the substrate to displace the halo-atom, mass spectrometral analysis of the structural changes during catalysis, overview Pseudomonas sp. ?
-
 ?
additional information the enzyme reaction mechanism proceeds via an ester intermediate and a nucleophilic aspartate attacking the C-atom of the substrate to displace the halo-atom, mass spectrometral analysis of the structural changes during catalysis, overview Pseudomonas sp. 113 ?
-
 ?

Subunits

Subunits Comment Organism
More docking and structure analysis of enzyme mutants K151A and D180A in complex with substrate (S)-2-chloropropionic acid, molecular dynamics, overview Pseudomonas sp.

Synonyms

Synonyms Comment Organism
L-2-haloacid dehalogenase
-
 Pseudomonas sp.
L-DEX YL
-
 Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information single turnover Pseudomonas sp.

General Information

General Information Comment Organism
evolution the enzyme belongs to the HAD superfamily Pseudomonas sp.