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Literature summary for 3.8.1.2 extracted from

  • Rye, C.A.; Isupov, M.N.; Lebedev, A.A.; Littlechild, J.A.
    Biochemical and structural studies of a L-haloacid dehalogenase from the thermophilic archaeon Sulfolobus tokodaii (2009), Extremophiles, 13, 179-190.
    View publication on PubMed
Search for more literature for 3.8.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Sulfurisphaera tokodaii

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition with up to 100 mM of D/L-lactate or pyruvate Sulfurisphaera tokodaii

Organic Solvent Stability

Organic Solvent Comment Organism
acetonitrile in acetonitrile the activity is reduced by over 70% for any concentration tested Sulfurisphaera tokodaii
DMSO the enzyme is relatively stable to solvents with 25% activity lost when incubated for 1 h in 20% (v/v) DMSO, at higher concentrations of DMSO the activity is reduced to below 40% of normal Sulfurisphaera tokodaii
Ethanol in 10% (v/v) ethanol activity is still 40% of normal, further increases in ethanol concentration reduce the activity until at 50% (v/v) ethanol less than 10% activity is observed Sulfurisphaera tokodaii
Methanol the activity in 10% (v/v) methanol is only 25% of normal, similar activity is observed for the higher concentrations of methanol Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q96XE7 201 amino acids long hypothetical 2-haloalkanoic acid dehalogenase
-
Sulfurisphaera tokodaii 7 Q96XE7 201 amino acids long hypothetical 2-haloalkanoic acid dehalogenase
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and gel filtration Sulfurisphaera tokodaii

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Sulfurisphaera tokodaii

Synonyms

Synonyms Comment Organism
L-haloacid dehalogenase
-
 Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Sulfurisphaera tokodaii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 90 the enzyme is stable to prolonged exposure to 50°C, losing only 8% activity after 90 min incubation, as the temperature is increased the stability decreases, the enzyme is thermostable with a half-life of over 1 h at 70°C Sulfurisphaera tokodaii