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Literature summary for 3.8.1.2 extracted from

  • Soda, K.; Kurihara, T.; Liu, J.Q.; Nardi-Dei, V.; Park, C.; Miyagi, M.; Tsunasawa, S.; Esaki, N.
    Bacterial 2-haloacid dehalogenases: structures and catalytic properties (1996), Pure Appl. Chem., 68(11), 2097-2103.
No PubMed abstract available
Search for more literature for 3.8.1.2

Application

Application Comment Organism
synthesis production of optically active 2-hydroxyalkanoic acids and 2-haloalkanoic acids for chiral synthesis in industry Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
p-mercuric chlorobenzoate
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000 28000
-
 Pseudomonas sp.
25000 28000
-
 Rhizobium sp.
25000 28000
-
 Xanthobacter autotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DL-2-haloalkanoic acid + H2O Rhizobium sp.
-
 L-2-hydroxyalkanoic acid + D-2-hydroxyalkanoic acid + halide
-
 ?
L-2-haloalkanoic acid + H2O Pseudomonas sp.
-
 D-2-hydroxyalkanoic acid + halide
-
 ?
L-2-haloalkanoic acid + H2O Rhizobium sp.
-
 D-2-hydroxyalkanoic acid + halide
-
 ?
L-2-haloalkanoic acid + H2O Xanthobacter autotrophicus
-
 D-2-hydroxyalkanoic acid + halide
-
 ?
monochloroacetate + H2O Pseudomonas sp.
-
 glycolate + HCl
-
 ?
monochloroacetate + H2O Rhizobium sp.
-
 glycolate + HCl
-
 ?
monochloroacetate + H2O Xanthobacter autotrophicus
-
 glycolate + HCl
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Rhizobium sp.
-
-
-
Xanthobacter autotrophicus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide Asp10 of the enzyme functions as a catalytic nucleophile: the residue attacks the alpha-carbon of the substrate to form an ester intermediate, which is subsequently hydrolysed to release the product Pseudomonas sp.
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-haloacid + H2O
-
 Pseudomonas sp. (R)-2-hydroxyacid + halide
-
 ?
(S)-2-haloacid + H2O
-
 Rhizobium sp. (R)-2-hydroxyacid + halide
-
 ?
(S)-2-haloacid + H2O
-
 Xanthobacter autotrophicus (R)-2-hydroxyacid + halide
-
 ?
DL-2-haloalkanoic acid + H2O
-
 Rhizobium sp. L-2-hydroxyalkanoic acid + D-2-hydroxyalkanoic acid + halide
-
 ?
L-2-chloropropionic acid + H2O
-
 Pseudomonas sp. D-2-hydroxypropionic acid + HCl
-
 ?
L-2-haloalkanoic acid + H2O
-
 Pseudomonas sp. D-2-hydroxyalkanoic acid + halide
-
 ?
L-2-haloalkanoic acid + H2O
-
 Rhizobium sp. D-2-hydroxyalkanoic acid + halide
-
 ?
L-2-haloalkanoic acid + H2O
-
 Xanthobacter autotrophicus D-2-hydroxyalkanoic acid + halide
-
 ?
monochloroacetate + H2O
-
 Pseudomonas sp. glycolate + HCl
-
 ?
monochloroacetate + H2O
-
 Rhizobium sp. glycolate + HCl
-
 ?
monochloroacetate + H2O
-
 Xanthobacter autotrophicus glycolate + HCl
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Pseudomonas sp.