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Literature summary for 3.8.1.10 extracted from

  • Liu, J.Q.; Kurihara, T.; Hasan, A.K.M.Q.; Nardi-Dei, V.; Koshikawa, H.; Esaki, N.; Soda, K.
    Purification and characterization of thermostable and nonthermostable 2-haloacid dehalogenases with different stereospecificities from Pseudomonas sp. strain YL (1994), Appl. Environ. Microbiol., 60, 2389-2393.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.8.1.10

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ HgSO4 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-2-haloacid + H2O Pseudomonas sp.
-
 (S)-2-hydroxyacid + halide
-
 ?
(S)-2-haloacid + H2O Pseudomonas sp.
-
 (R)-2-hydroxyacid + halide
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-haloacid + H2O
-
 Pseudomonas sp. (S)-2-hydroxyacid + halide
-
 ?
(S)-2-haloacid + H2O
-
 Pseudomonas sp. (R)-2-hydroxyacid + halide
-
 ?
D-2-monochloropropionate + H2O
-
 Pseudomonas sp. L-lactate + HCl
-
 ?
L-2-monochloropropionate + H2O
-
 Pseudomonas sp. D-lactate + HCl
-
 ?
monobromoacetate + H2O
-
 Pseudomonas sp. glycolate + HBr
-
 ?
monochloroacetate + H2O
-
 Pseudomonas sp. glycolate + HCl
-
 ?
monoiodoacetate + H2O
-
 Pseudomonas sp. glycolate + HI
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 36000 SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 30 min 80% loss of activity Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
-
 Pseudomonas sp.