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Literature summary for 3.8.1.10 extracted from

  • Motosugi, K; Esahi, N.; Soda, K.
    Purification and properties of a new enzyme, DL-2-haloacid dehalogenase, from Pseudomonas sp. (1982), J. Bacteriol., 150, 522-527.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.8.1.10

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ HgCl2 Pseudomonas sp.
HgCl2
-
 Pseudomonas sp.
Mn2+ MnSO4 Pseudomonas sp.
additional information not: thiol reagents Pseudomonas sp.
thiol reagents not Pseudomonas sp.
Zn2+ ZnSO4 Pseudomonas sp.
ZnSO4
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 L-2-chloropropionate
-
 Pseudomonas sp.
3.2
-
 DL-2-chloropropionate
-
 Pseudomonas sp.
4.8
-
 D-2-chloropropionate
-
 Pseudomonas sp.
5
-
 Monochloroacetate pH 9.5, 30°C Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000, SDS-PAGE Pseudomonas sp.
68000
-
 sedimentation equilibrium Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-2-haloacid + H2O Pseudomonas sp.
-
 (S)-2-hydroxyacid + halide
-
 ?
(S)-2-haloacid + H2O Pseudomonas sp.
-
 (R)-2-hydroxyacid + halide
-
 ?
(S)-2-haloacid + H2O Pseudomonas sp. 113
-
 (R)-2-hydroxyacid + halide
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. 113
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Storage Stability

Storage Stability Organism
-20°C, 25 mM potassium phosphate buffer containing 50% glycerol, pH 7.5, 1 year Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-haloacid + H2O
-
 Pseudomonas sp. (S)-2-hydroxyacid + halide
-
 ?
(S)-2-haloacid + H2O
-
 Pseudomonas sp. (R)-2-hydroxyacid + halide
-
 ?
(S)-2-haloacid + H2O
-
 Pseudomonas sp. 113 (R)-2-hydroxyacid + halide
-
 ?
D-2-monochloropropionate + H2O
-
 Pseudomonas sp. L-lactate + HCl
-
 ?
DL-2-chloropropionate + H2O
-
 Pseudomonas sp. DL-lactate + HCl
-
 ?
monobromoacetate + H2O
-
 Pseudomonas sp. glycolate + HBr
-
 ?
monobromoacetate + H2O
-
 Pseudomonas sp. 113 glycolate + HBr
-
 ?
monochloroacetate + H2O
-
 Pseudomonas sp. glycolate + HCl
-
 ?
monochloroacetate + H2O
-
 Pseudomonas sp. 113 glycolate + HCl
-
 ?
monoiodoacetate + H2O
-
 Pseudomonas sp. glycolate + HI
-
 ?
monoiodoacetate + H2O
-
 Pseudomonas sp. 113 glycolate + HI
-
 ?
additional information not: chloroacetamide, chloroacetaldehyde, 3-chloropropionate, best substrate: 2-halopropionate, followed by monohaloacetate, 2-halobutyrate, and 2-halovalerate in that order Pseudomonas sp. ?
-
 ?
additional information not: chloroacetamide, chloroacetaldehyde, 3-chloropropionate, best substrate: 2-halopropionate, followed by monohaloacetate, 2-halobutyrate, and 2-halovalerate in that order Pseudomonas sp. 113 ?
-
 ?
trichloroacetate + H2O
-
 Pseudomonas sp. ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 35000, SDS-PAGE Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 10
-
 Pseudomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.28
-
 HgCl2 pH 9.5, 30°C Pseudomonas sp.
0.51
-
 ZnSO4 pH 9.5, 30°C Pseudomonas sp.