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Literature summary for 3.7.1.9 extracted from

  • Betz, A.; Facey, S.J.; Hauer, B.; Tshisuaka, B.; Lingens, F.
    Molecular cloning, sequencing, expression, and site-directed mutagenesis of the 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase gene from Arthrobacter spec. Rue61a (2000), J. Basic Microbiol., 40, 7-23.
    View publication on PubMed
Search for more literature for 3.7.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli XL1-Blue M15 Arthrobacter sp.

Protein Variants

Protein Variants Comment Organism
D233A residual activity of 62.6% Arthrobacter sp.
S101A residual activity of 43.1% Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
 1H-3-Hydroxy-4-oxoquinaldine His-tagged HOD Arthrobacter sp.
0.0325
-
 1H-3-Hydroxy-4-oxoquinaldine mutant S101A Arthrobacter sp.
0.044
-
 1H-3-Hydroxy-4-oxoquinaldine mutant D233A Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31840
-
 sequence analysis Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
 Rue61a
-
Arthrobacter sp. Rue61a
-
 Rue61a
-

Purification (Commentary)

Purification (Comment) Organism
His-tagged HOD purified by Ni2+/NTA affinity chromatography Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.1
-
 mutant S101A Arthrobacter sp.
17.6
-
 mutant D233A Arthrobacter sp.
28.1
-
 His-tagged HOD Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1H-3-hydroxy-4-oxoquinaldine + H2O
-
 Arthrobacter sp. ?
-
 ?
1H-3-hydroxy-4-oxoquinaldine + H2O
-
 Arthrobacter sp. Rue61a ?
-
 ?

Synonyms

Synonyms Comment Organism
1H-3-Hydroxy-4-oxoquinaldine 2,4-dioxygenase
-
 Arthrobacter sp.
HOD
-
 Arthrobacter sp.