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Literature summary for 3.7.1.9 extracted from

  • Jun, S.; Fushinobu, S.; Nojiri, H.; Omori, T.; Shoun, H.; Wakagi, T.
    Improving the catalytic efficiency of a meta-cleavage product hydrolase (CumD) from Pseudomonas fluorescens IP01 (2006), Biochim. Biophys. Acta, 1764, 1159-1166.
    View publication on PubMed
Search for more literature for 3.7.1.9

Crystallization (Commentary)

Crystallization (Comment) Organism
A129V mutant, at 1.65 A resolution, crystals of the A129V mutant isomorphous with the S103A crystals Pseudomonas fluorescens

Protein Variants

Protein Variants Comment Organism
A129V improved catalytic efficiency than the wild type enzyme, possesses a more appropiate binding pocket for substrates with smaller C6 substituents Pseudomonas fluorescens
A129V/I199V/V227I combination of mutations incompatible for correct folding Pseudomonas fluorescens
F104M mutation does not have any significant effect on enzyme characteristics Pseudomonas fluorescens
I199V improved catalytic efficiency than the wild type enzyme Pseudomonas fluorescens
I199V/V2271 combination of mutations incompatible for correct folding Pseudomonas fluorescens
S34A lower Km and turnover rates compared to wild-type Pseudomonas fluorescens
S34G lower Km and turnover rates compared to wild-type Pseudomonas fluorescens
V227I improved catalytic efficiency than the wild type enzyme Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00045
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate mutant I199V Pseudomonas fluorescens
0.0008
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant S34G Pseudomonas fluorescens
0.0014
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant I199V Pseudomonas fluorescens
0.0017
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate mutant A129/I199V Pseudomonas fluorescens
0.0018
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant A129V Pseudomonas fluorescens
0.002
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant V227I Pseudomonas fluorescens
0.0022
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate wild-type Pseudomonas fluorescens
0.0023
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant I199V Pseudomonas fluorescens
0.0024
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant A129/V227I Pseudomonas fluorescens
0.0025
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant S34A Pseudomonas fluorescens
0.0026
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant S34G Pseudomonas fluorescens
0.0027
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant F104M Pseudomonas fluorescens
0.0028
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant V227I Pseudomonas fluorescens
0.0031
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant S34A Pseudomonas fluorescens
0.0035
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant A129/I199V Pseudomonas fluorescens
0.0035
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant I199V Pseudomonas fluorescens
0.0039
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant F104M Pseudomonas fluorescens
0.004
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant F104M Pseudomonas fluorescens
0.0042
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant S34G Pseudomonas fluorescens
0.0048
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant A129/I199V Pseudomonas fluorescens
0.0049
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate mutant V227I Pseudomonas fluorescens
0.0049
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant V227I Pseudomonas fluorescens
0.005
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant A129/I199V Pseudomonas fluorescens
0.005
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant S34A Pseudomonas fluorescens
0.0055
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant A129/V227I Pseudomonas fluorescens
0.0056
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant A129V Pseudomonas fluorescens
0.0058
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate mutant A129/V227I Pseudomonas fluorescens
0.0078
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate wild-type Pseudomonas fluorescens
0.0089
-
 2-hydroxy-6-oxohepta-2,4-dienoate wild-type Pseudomonas fluorescens
0.0098
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant A129V Pseudomonas fluorescens
0.01
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant A129/V227I Pseudomonas fluorescens
0.01
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate mutant A129V Pseudomonas fluorescens
0.011
-
 2-hydroxy-6-oxoocta-2,4-dienoate wild-type Pseudomonas fluorescens
0.015
-
 2-hydroxy-6-oxoocta-2,4-dienoate
-
 Pseudomonas putida
0.017
-
 2-hydroxy-6-oxohepta-2,4-dienoate
-
 Pseudomonas putida
0.022
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate
-
 Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
 IP01
-
Pseudomonas fluorescens IP01
-
 IP01
-
Pseudomonas putida
-
 F1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 Pseudomonas fluorescens ?
-
 ?
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 Pseudomonas fluorescens IP01 ?
-
 ?
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate + H2O
-
 Pseudomonas fluorescens ?
-
 ?
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate + H2O
-
 Pseudomonas putida ?
-
 ?
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate + H2O
-
 Pseudomonas fluorescens IP01 ?
-
 ?
2-hydroxy-6-oxohepta-2,4-dienoate + H2O
-
 Pseudomonas fluorescens ?
-
 ?
2-hydroxy-6-oxohepta-2,4-dienoate + H2O
-
 Pseudomonas putida ?
-
 ?
2-hydroxy-6-oxohepta-2,4-dienoate + H2O
-
 Pseudomonas fluorescens IP01 ?
-
 ?
2-hydroxy-6-oxoocta-2,4-dienoate + H2O
-
 Pseudomonas fluorescens ?
-
 ?
2-hydroxy-6-oxoocta-2,4-dienoate + H2O
-
 Pseudomonas putida ?
-
 ?
2-hydroxy-6-oxoocta-2,4-dienoate + H2O
-
 Pseudomonas fluorescens IP01 ?
-
 ?
additional information can not hydrolyze 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate Pseudomonas fluorescens ?
-
 ?
additional information can not hydrolyze 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate Pseudomonas putida ?
-
 ?
additional information can not hydrolyze 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate Pseudomonas fluorescens IP01 ?
-
 ?

Synonyms

Synonyms Comment Organism
CumD
-
 Pseudomonas fluorescens
meta-cleavage product hydrolase
-
 Pseudomonas fluorescens
meta-cleavage product hydrolase
-
 Pseudomonas putida
TodF
-
 Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate mutant A129/V227I Pseudomonas fluorescens
0.01
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate mutant A129/I199V Pseudomonas fluorescens
0.01
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate mutant A129V Pseudomonas fluorescens
0.02
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate mutant I199V Pseudomonas fluorescens
0.02
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate mutant V227I Pseudomonas fluorescens
0.037
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate wild-type Pseudomonas fluorescens
0.3
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant S34A Pseudomonas fluorescens
0.41
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate
-
 Pseudomonas putida
0.73
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant A129/V227I Pseudomonas fluorescens
1.3
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant S34A Pseudomonas fluorescens
1.5
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant S34G Pseudomonas fluorescens
3.3
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant A129/I199V Pseudomonas fluorescens
4.55
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant A129/I199V Pseudomonas fluorescens
6.4
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant F104M Pseudomonas fluorescens
6.5
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant F104M Pseudomonas fluorescens
6.7
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant S34A Pseudomonas fluorescens
8.1
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant S34G Pseudomonas fluorescens
8.4
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant S34G Pseudomonas fluorescens
14
-
 2-hydroxy-6-oxoocta-2,4-dienoate
-
 Pseudomonas putida
15.4
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant F104M Pseudomonas fluorescens
19
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant A129/I199V Pseudomonas fluorescens
19.5
-
 2-hydroxy-6-oxohepta-2,4-dienoate wild-type Pseudomonas fluorescens
20
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant V227I Pseudomonas fluorescens
23.1
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate wild-type Pseudomonas fluorescens
24
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant A129/V227I Pseudomonas fluorescens
25
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant A129V Pseudomonas fluorescens
29
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant A129/V227I Pseudomonas fluorescens
29
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant I199V Pseudomonas fluorescens
35
-
 2-hydroxy-6-oxohepta-2,4-dienoate
-
 Pseudomonas putida
35
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant A129/I199V Pseudomonas fluorescens
37
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant I199V Pseudomonas fluorescens
38
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant I199V Pseudomonas fluorescens
42.4
-
 2-hydroxy-6-oxoocta-2,4-dienoate wild-type Pseudomonas fluorescens
52
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant V227I Pseudomonas fluorescens
75
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant A129V Pseudomonas fluorescens
78
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant V227I Pseudomonas fluorescens
81
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant A129V Pseudomonas fluorescens