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Literature summary for 3.7.1.9 extracted from

  • Khajamohiddin, S.; Babu, P.S.; Chakka, D.; Merrick, M.; Bhaduri, A.; Sowdhamini, R.; Siddavattam, D.
    A novel meta-cleavage product hydrolase from Flavobacterium sp. ATCC27551 (2006), Biochem. Biophys. Res. Commun., 351, 675-681.
    View publication on PubMed
Search for more literature for 3.7.1.9

Activating Compound

Activating Compound Comment Organism Structure
additional information EDTA has no effect Flavobacterium sp.

Application

Application Comment Organism
molecular biology orf234, encoding an alpha/beta hydrolase, which is distantly related to the meta-fission product hydrolases such as XylF, PhnD, and CumD Flavobacterium sp.

Cloned(Commentary)

Cloned (Comment) Organism
overexpression as an N-terminal His-tag from pSM12 in Escherichia coli BL21(DE3), or from plasmid pSM13 in Pseudomonas aeruginosa PAO1161 Flavobacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
DEPC inhibits by 82% Flavobacterium sp.
additional information N-ethylmaleimide and p-chloromercuribenzoate show no significant effect Flavobacterium sp.
PMSF inhibits by 75% Flavobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0048
-
 2-hydroxy-6-oxohepta-2,4-dienoate
-
 Flavobacterium sp.
0.0057
-
 2-hydroxymuconic semialdehyde
-
 Flavobacterium sp.
0.0074
-
 2-hydroxy-5-methylmuconic semialdehyde
-
 Flavobacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Fe2+, Ca2+, Cu2+, Mg2+, Mn2+, Co2+, Li2+, and Zn2+ have no effect Flavobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 4 * 30000, SDS-PAGE Flavobacterium sp.
120000
-
 gel filtration Flavobacterium sp.

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
 ATCC27551
-
Flavobacterium sp. ATCC 27551
-
 ATCC27551
-

Purification (Commentary)

Purification (Comment) Organism
by affinity chromatography Flavobacterium sp.

Storage Stability

Storage Stability Organism
-20°C, 20% glycerol, 15 days Flavobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-5-methylmuconic semialdehyde + H2O
-
 Flavobacterium sp. ?
-
 ?
2-hydroxy-5-methylmuconic semialdehyde + H2O
-
 Flavobacterium sp. ATCC 27551 ?
-
 ?
2-hydroxy-6-oxohepta-2,4-dienoate + H2O
-
 Flavobacterium sp. ?
-
 ?
2-hydroxy-6-oxohepta-2,4-dienoate + H2O
-
 Flavobacterium sp. ATCC 27551 ?
-
 ?
2-hydroxymuconic semialdehyde + H2O
-
 Flavobacterium sp. ?
-
 ?
2-hydroxymuconic semialdehyde + H2O
-
 Flavobacterium sp. ATCC 27551 ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 30000, SDS-PAGE Flavobacterium sp.

Synonyms

Synonyms Comment Organism
meta-cleavage product hydrolase
-
 Flavobacterium sp.
MfhA
-
 Flavobacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
 loses just 20% activity after incubation for 2 hours Flavobacterium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 85 above 75°C enzyme activity declines rapidly Flavobacterium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16
-
 2-hydroxy-5-methylmuconic semialdehyde
-
 Flavobacterium sp.
42
-
 2-hydroxymuconic semialdehyde
-
 Flavobacterium sp.
57
-
 2-hydroxy-6-oxohepta-2,4-dienoate
-
 Flavobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Flavobacterium sp.