Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.7.1.9 extracted from

  • Duggleby, C.J.; Williams, P.A.
    Purification and some properties of the 2-hydroxy-6-oxohepta-2,4-dienoate hydrolase (2-hydroxymuconic semialdehyde hydrolase) encoded by the TOL plasmid pWW0 from Pseudomonas putida mt-2 (1986), J. Gen. Microbiol., 132, 717-726.
No PubMed abstract available
Search for more literature for 3.7.1.9

Inhibitors

Inhibitors Comment Organism Structure
AgNO3
-
 Pseudomonas putida
CuCl2
-
 Pseudomonas putida
iodoacetamide
-
 Pseudomonas putida
PCMB
-
 Pseudomonas putida
sodium arsenite
-
 Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-propylcatechol
-
 Pseudomonas putida
0.01
-
 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-methylcatechol
-
 Pseudomonas putida
0.015
-
 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-ethylcatechol
-
 Pseudomonas putida
0.022
-
 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-ethylcatechol
-
 Pseudomonas putida
0.023
-
 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-propylcatechol
-
 Pseudomonas putida
0.03
-
 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon catechol
-
 Pseudomonas putida
0.036
-
 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-methylcatechol
-
 Pseudomonas putida
0.037
-
 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-allylcatechol
-
 Pseudomonas putida
0.122
-
 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-chlorocatechol
-
 Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas putida enzyme is part of the pathway for the catabolism of toluene and m-xylene and p-xylene ?
-
 ?
additional information Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 enzyme is part of the pathway for the catabolism of toluene and m-xylene and p-xylene ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
48
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-6-oxohepta-2,4-dienoate + H2O
-
 Pseudomonas putida ?
-
 ?
2-hydroxy-6-oxohepta-2,4-dienoate + H2O
-
 Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 ?
-
 ?
2-hydroxymuconate semialdehyde + H2O
-
 Pseudomonas putida formate + 2-oxopent-4-enoate
-
 ?
2-hydroxymuconate semialdehyde + H2O
-
 Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 formate + 2-oxopent-4-enoate
-
 ?
3-allylcatechol ring-fission product + H2O meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-allylcatechol Pseudomonas putida ?
-
 ?
3-ethylcatechol ring-fission product + H2O meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-ethylcatechol Pseudomonas putida ?
-
 ?
3-methylcatechol ring-fission product + H2O meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-methylcatechol Pseudomonas putida ?
-
 ?
3-methylcatechol ring-fission product + H2O meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-methylcatechol Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 ?
-
 ?
3-propylcatechol ring-fission product + H2O meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-propylcatechol Pseudomonas putida ?
-
 ?
4-chlorocatechol ring-fission product + H2O meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-chlorocatechol Pseudomonas putida ?
-
 ?
4-ethylcatechol ring-fission product + H2O meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-ethylcatechol Pseudomonas putida ?
-
 ?
4-methylcatechol ring-fission product + H2O meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-methylcatechol Pseudomonas putida ?
-
 ?
4-propylcatechol ring-fission product + H2O meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-propylcatechol Pseudomonas putida ?
-
 ?
catechol ring-fission product + H2O meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon catechol Pseudomonas putida ?
-
 ?
catechol ring-fission product + H2O meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon catechol Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 ?
-
 ?
meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-allylcatechol + H2O
-
 Pseudomonas putida ?
-
 ?
meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-ethylcatechol + H2O
-
 Pseudomonas putida ?
-
 ?
meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-methylcatechol + H2O
-
 Pseudomonas putida ?
-
 ?
meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-propylcatechol + H2O
-
 Pseudomonas putida ?
-
 ?
meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-chlorocatechol + H2O
-
 Pseudomonas putida ?
-
 ?
meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-ethylcatechol + H2O
-
 Pseudomonas putida ?
-
 ?
meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-methylcatechol + H2O
-
 Pseudomonas putida ?
-
 ?
meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-propylcatechol + H2O
-
 Pseudomonas putida ?
-
 ?
meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon catechol + H2O
-
 Pseudomonas putida ?
-
 ?
additional information enzyme is part of the pathway for the catabolism of toluene and m-xylene and p-xylene Pseudomonas putida ?
-
 ?
additional information enzyme is part of the pathway for the catabolism of toluene and m-xylene and p-xylene Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 31000-33000, SDS-PAGE after denaturation with 2-mercaptoethanol and urea Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
 Pseudomonas putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 8 optimal stability at pH 7-8, rapid loss of activity below pH 5 and above pH 9.5 Pseudomonas putida