Protein Variants | Comment | Organism |
---|---|---|
C254S | 1% of the activity of wild-type enzyme, expressed at lower levels than the other mutant enzymes | Pseudomonas putida |
D228A | activity below detection | Pseudomonas putida |
D65V | activity below detection. Unstable, decreases in cellular extrects after 10 min | Pseudomonas putida |
F108M | 18% of the activity of wild-type enzyme, enzyme displays greater thermostability than the wild-type enzyme | Pseudomonas putida |
H249A | 26% of the activity of wild-type enzyme, expressed at lower levels than the other mutant enzymes, severe decrease in protein stability compared to wild-type enzyme | Pseudomonas putida |
H256A | activity below detection | Pseudomonas putida |
H36A | 15% of the activity of wild-type enzyme, severe decrease in protein stability over that of the wild-type enzyme | Pseudomonas putida |
S107A | activity below detection | Pseudomonas putida |
S107C | 0.44% of the activity of wild-type enzyme | Pseudomonas putida |
S152A | 80% of the activity of wild-type enzyme, severe decrease in protein stability over that of the wild-type enzyme | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-dichloroisocoumarin | - |
Pseudomonas putida | |
diethyl dicarbonate | - |
Pseudomonas putida | |
diisopropyl fluorophosphate | - |
Pseudomonas putida | |
NEM | - |
Pseudomonas putida | |
phenylmethylsulfonyl fluoride | - |
Pseudomonas putida | |
tosyl-Phe chloromethyl ketone | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0075 | - |
3-methylcatechol ring-fission product | mutant F108M | Pseudomonas putida | |
0.0076 | - |
3-methylcatechol ring-fission product | mutant S152A | Pseudomonas putida | |
0.0134 | - |
3-methylcatechol ring-fission product | mutant H249A | Pseudomonas putida | |
0.0136 | - |
3-methylcatechol ring-fission product | - |
Pseudomonas putida | |
0.023 | - |
3-methylcatechol ring-fission product | mutant H36A | Pseudomonas putida | |
0.085 | - |
catechol ring-fission product | - |
Pseudomonas putida | |
0.14 | - |
catechol ring-fission product | mutant H36A | Pseudomonas putida | |
0.155 | - |
catechol ring-fission product | mutant S152A | Pseudomonas putida | |
0.231 | - |
catechol ring-fission product | mutant H249A | Pseudomonas putida | |
0.35 | - |
catechol ring-fission product | mutant F108M | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas putida | enzyme of the hydrolytic branch of the meta-cleavage pathway for the catabolism of catechol and alkyl-substituted catechols | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylcatechol ring-fission product + H2O | - |
Pseudomonas putida | ? | - |
? | |
catechol ring-fission product + H2O | - |
Pseudomonas putida | ? | - |
? | |
additional information | enzyme of the hydrolytic branch of the meta-cleavage pathway for the catabolism of catechol and alkyl-substituted catechols | Pseudomonas putida | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
wild-type enzyme loses 50% of its activity after 15 min, mutant enzyme H36A loses 50% of its initial activity after 1.6 min, mutant enzyme F108M loses 60% of its initial activity after 60 min, mutant enzyme S152A loses 50% of its initial activity after 2.2 min, mutant enzyme H249A loses 50% of its initial activity after 1.1 min | Pseudomonas putida |