Application | Comment | Organism |
---|---|---|
environmental protection | potential enzyme resource for the biodegradation of biphenyl, bioremediation of the environmental pollution caused by biphenyl/polychlorinated biphenyls | Arthrobacter sp. YC-RL1 |
Cloned (Comment) | Organism |
---|---|
heterologously expressed in Escherichia coli BL21 (DE3) | Arthrobacter sp. YC-RL1 |
Protein Variants | Comment | Organism |
---|---|---|
D247A | mutant enzyme retains 29.93% enzyme activity | Arthrobacter sp. YC-RL1 |
H275A | mutant enzyme retains 10.25% enzyme activity | Arthrobacter sp. YC-RL1 |
S115A | mutant enzyme retains 28.92% enzyme activity | Arthrobacter sp. YC-RL1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CHAPS | - |
Arthrobacter sp. YC-RL1 | |
DEPC | - |
Arthrobacter sp. YC-RL1 | |
DTT | - |
Arthrobacter sp. YC-RL1 | |
additional information | insensitive to EDTA | Arthrobacter sp. YC-RL1 | |
NBS | - |
Arthrobacter sp. YC-RL1 | |
PMSF | - |
Arthrobacter sp. YC-RL1 | |
SDS | - |
Arthrobacter sp. YC-RL1 | |
Trition X-100 | - |
Arthrobacter sp. YC-RL1 | |
Tween 20 | - |
Arthrobacter sp. YC-RL1 | |
Tween 80 | - |
Arthrobacter sp. YC-RL1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
2-hydroxy-6-oxo-6-(2-hydroxyphenyl)-hexa-2,4-dienoate | pH 7.0, 30°C | Arthrobacter sp. YC-RL1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-6-oxo-6-(2-hydroxyphenyl)-hexa-2,4-dienoate + H2O | Arthrobacter sp. YC-RL1 | the enzyme is involved in the biodegradation of biphenyl | 2-oxopent-4-enoate + salicylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. YC-RL1 | A0A0H0ZQK0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Arthrobacter sp. YC-RL1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-6-oxo-6-(2-hydroxyphenyl)-hexa-2,4-dienoate + H2O | - |
Arthrobacter sp. YC-RL1 | 2-oxopent-4-enoate + salicylate | - |
? | |
2-hydroxy-6-oxo-6-(2-hydroxyphenyl)-hexa-2,4-dienoate + H2O | the enzyme is involved in the biodegradation of biphenyl | Arthrobacter sp. YC-RL1 | 2-oxopent-4-enoate + salicylate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-hydroxy-6-oxo-6-phenylhexa-2, 4-dienoate hydrolase | - |
Arthrobacter sp. YC-RL1 |
BphD | - |
Arthrobacter sp. YC-RL1 |
HOPDA hydrolase | - |
Arthrobacter sp. YC-RL1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 50 | - |
Arthrobacter sp. YC-RL1 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 55 | 15°C: about 45% of maximal activity, 55°C: about 70% of maximal activity | Arthrobacter sp. YC-RL1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
1 h, the enzyme retains more than 60% residual activity | Arthrobacter sp. YC-RL1 |
60 | - |
1 h, the enzyme retains less than 60% residual activity | Arthrobacter sp. YC-RL1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.61 | - |
2-hydroxy-6-oxo-6-(2-hydroxyphenyl)-hexa-2,4-dienoate | pH 7.0, 30°C | Arthrobacter sp. YC-RL1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Arthrobacter sp. YC-RL1 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | pH 5.0: about 65% of maximal activity, pH 9.0: about 85% of maximal activity, pH 10.0: about 35 % of maximal activity | Arthrobacter sp. YC-RL1 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | more than 60% of the activity is retained after incubation at pH 5.0-9.0 at 30°C for 10 min | Arthrobacter sp. YC-RL1 |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the biodegradation of biphenyl | Arthrobacter sp. YC-RL1 |