Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.7.1.8 extracted from

  • Riddle, R.R.; Gibbs, P.R.; Willson, R.C.; Benedik, M.J.
    Purification and properties of 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid hydrolase involved in microbial degradation of carbazole (2003), Protein Expr. Purif., 28, 182-189.
    View publication on PubMed

Application

Application Comment Organism
molecular biology the deduced amino acid sequence of CarC shows 30.3, 31.3, and 31.8% identity with meta-cleavage compound hydrolases TodF, XylF, and DmpD, respectively, from other Pseudomonas Pseudomonas sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp.

Protein Variants

Protein Variants Comment Organism
S114A ca. 40fold reduction in activity for the His tagged mutant hydrolase relative to similar lysates of His-tagged native CarC Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0046
-
2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid at 25°C Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32231
-
2 * 32231, native enzyme, predicted from peptide sequence Pseudomonas sp.
55000
-
gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
LD2
-
Pseudomonas sp. LD2
-
LD2
-

Purification (Commentary)

Purification (Comment) Organism
by metal affinity chromatography Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2600
-
in 0.05 mM 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid, 50 mM Tris, pH 7.5 at 25°C Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid + H2O
-
Pseudomonas sp. 2-aminobenzoic acid + 2-hydroxypenta-2,4-dienoic acid
-
?
2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid + H2O
-
Pseudomonas sp. LD2 2-aminobenzoic acid + 2-hydroxypenta-2,4-dienoic acid
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 32231, native enzyme, predicted from peptide sequence Pseudomonas sp.

Synonyms

Synonyms Comment Organism
2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid hydrolase
-
Pseudomonas sp.
CarC
-
Pseudomonas sp.
HOPDA hydrolase
-
Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
58
-
-
Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1300
-
2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid
-
Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10.5
-
Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor requirement Pseudomonas sp.