Application | Comment | Organism |
---|---|---|
molecular biology | the deduced amino acid sequence of CarC shows 30.3, 31.3, and 31.8% identity with meta-cleavage compound hydrolases TodF, XylF, and DmpD, respectively, from other Pseudomonas | Pseudomonas sp. |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas sp. |
Protein Variants | Comment | Organism |
---|---|---|
S114A | ca. 40fold reduction in activity for the His tagged mutant hydrolase relative to similar lysates of His-tagged native CarC | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0046 | - |
2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid | at 25°C | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32231 | - |
2 * 32231, native enzyme, predicted from peptide sequence | Pseudomonas sp. |
55000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
LD2 | - |
Pseudomonas sp. LD2 | - |
LD2 | - |
Purification (Comment) | Organism |
---|---|
by metal affinity chromatography | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2600 | - |
in 0.05 mM 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid, 50 mM Tris, pH 7.5 at 25°C | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid + H2O | - |
Pseudomonas sp. | 2-aminobenzoic acid + 2-hydroxypenta-2,4-dienoic acid | - |
? | |
2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid + H2O | - |
Pseudomonas sp. LD2 | 2-aminobenzoic acid + 2-hydroxypenta-2,4-dienoic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 32231, native enzyme, predicted from peptide sequence | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid hydrolase | - |
Pseudomonas sp. |
CarC | - |
Pseudomonas sp. |
HOPDA hydrolase | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
58 | - |
- |
Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1300 | - |
2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid | - |
Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10.5 | - |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor requirement | Pseudomonas sp. |