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Literature summary for 3.7.1.8 extracted from

  • Speare, D.M.; Fleming, S.M.; Beckett, M.N.; Li, J.J.; Bugg, T.D.
    Synthetic 6-aryl-2-hydroxy-6-ketohexa-2,4-dienoic acid substrates for C-C hydrolase BphD: investigation of a general base catalytic mechanism (2004), Org. Biomol. Chem., 2, 2942-2950.
    View publication on PubMed
Search for more literature for 3.7.1.8

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli BL21(DE3)(pLysS1)/pAIA51 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information values between 0.00013 and 0.00106 mM for processing of the 6-acryl-2-hydroxy-6-ketohexa-2,4-dienoic acid substrates Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 LB400
-
Pseudomonas sp. LB400
-
 LB400
-

Purification (Commentary)

Purification (Comment) Organism
to more than 90% homogeneitiy Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4
-
 purified enzyme Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dihydroxy-6-phenylhexa-2,4-dienoate
-
 Pseudomonas sp. benzaldehyde + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
2,6-dihydroxy-6-phenylhexa-2,4-dienoate
-
 Pseudomonas sp. LB400 benzaldehyde + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
additional information 6-acryl-2-hydroxy-6-ketohexa-2,4-dienoic acids synthesized as substrates for C-C hydrolase BphD Pseudomonas sp. ?
-
 ?
additional information 6-acryl-2-hydroxy-6-ketohexa-2,4-dienoic acids synthesized as substrates for C-C hydrolase BphD Pseudomonas sp. LB400 ?
-
 ?

Synonyms

Synonyms Comment Organism
BphD
-
 Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information values between 0.88 and 5.85 s-1 for processing of the 6-acryl-2-hydroxy-6-ketohexa-2,4-dienoic acid substrates Pseudomonas sp.