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Literature summary for 3.7.1.4 extracted from

  • Minamikawa, T.; Jayasankar, N.P.; Bohm, B.A.; Taylor, I.E.P.; Towers, G.H.N.
    An inducible hydrolase from Aspergillus niger, acting on carbon-carbon bonds, for phlorrhizin and other C-acylated phenols (1970), Biochem. J., 116, 889-897.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.7.1.4

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 0.02 mM Aspergillus niger
Al2(SO4)3 1 mM Aspergillus niger
Cd2+ 1 mM Aspergillus niger
Co2+ 1 mM Aspergillus niger
Cu2+ 1 mM Aspergillus niger
Fe3+ 1 mM Aspergillus niger
Hg2+ 0.02 mM Aspergillus niger
L-cysteine in presence of p-chloromercuribenzoate, 0.05 mM Aspergillus niger
Na2MoO4 1 mM Aspergillus niger
Na2WO4 1 mM Aspergillus niger
p-chloromercuribenzoate 0.05 mM Aspergillus niger
Zn2+ 0.2 mM Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 2-chloro-1-(3-methylphenyl)ethanone
-
 Aspergillus niger
0.3 0.4 phloretin
-
 Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
mycelial felt
-
 Aspergillus niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6
-
-
 Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3,4',6'-tetrahydroxy-4-methoxydihydrochalcone + H2O
-
 Aspergillus niger phloroglucinol + ?
-
 ?
2',3,4',6'-tetrahydroxy-dihydrochalcone + H2O
-
 Aspergillus niger phloroglucinol + ?
-
 ?
2',3,4,4',6'-pentahydroxy-dihydrochalcone 2'-beta-D-glucoside + H2O
-
 Aspergillus niger phloroglucinol beta-D-glucoside + 3-(3,4-dihydroxyphenyl)propanoic acid
-
 ?
2',4'-dihydroxy-4-methoxydihydrochalcone + H2O
-
 Aspergillus niger resorcinol + ?
-
 ?
2',4'-dihydroxypropiophenone + H2O
-
 Aspergillus niger resorcinol
-
 ?
2',4,4',6'-tetrahydroxy-dihydrochalcone + H2O
-
 Aspergillus niger phloroglucinol + ?
-
 ?
2',4,4'-trihydroxyhydrochalcone
-
 Aspergillus niger ?
-
 ?
2',6'-dihydroxy-4,4'-dimethoxydihydrochalcone + H2O
-
 Aspergillus niger 3-methoxyresorcinol + ?
-
 ?
3'-methylchloracetophenone + H2O
-
 Aspergillus niger ?
-
 ?
chloracetophenone + H2O
-
 Aspergillus niger ?
-
 ?
phloretin + H2O
-
 Aspergillus niger phloretate + phloroglucinol
-
 ?
phlorhizin + H2O
-
 Aspergillus niger 3-(4-hydroxyphenyl)propanoate + arbutin
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-20
-
 after 4 weeks loss of 20% activity Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.6
-
-
 Aspergillus niger