Cloned (Comment) | Organism |
---|---|
expression in Spodoptera frugiperda cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,7-dihydroxy-deamino-DL-kynurenine | mixed inhibition | Homo sapiens | |
D-kynurenine | mixed inhibition | Homo sapiens | |
L-kynurenine | competitive inhibition, at low substrate concentrations; non-competitive inhibition, at higher levels of substrate | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
3-hydroxy-L-kynurenine | pH 7.9, at 37ºC | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52400 | - |
recombinant enzyme, MALDI-TOF MS | Homo sapiens |
52500 | - |
native enzyme, 2 * 52500, disc gel electrophoresis | Homo sapiens |
95000 | - |
native enzyme, disc gel electrophoresis without SDS and mercaptoethanol | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-L-kynurenine + H2O | Homo sapiens | L-tryptophan metabolism, biosynthesis of nicotinamide nucleotides | 3-hydroxyanthranilate + L-alanine | - |
? | |
L-kynurenine + H2O | Homo sapiens | - |
anthranilate + L-alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, ammonium sulfate precipitation, DEAE-Sepharose CL-6B affinity column, hydroxyapatite column, strong anion-exchange column at pH 8.6, strong cation-exchange column at pH 6.0 strong anion-exchange column at pH 6.0, 60fold | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.164 | - |
pH 7.9, 37ºC | Homo sapiens |
Storage Stability | Organism |
---|---|
-80ºC, pyridoxal 5'-phosphate, more than 12 months, no loss of activity | Homo sapiens |
-80ºC, without pyridoxal 5'-phosphate, more than 12 months, no loss of activity | Homo sapiens |
4ºC, pyridoxal 5'-phosphate, up to 2 weeks, stable | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-L-kynurenine + H2O | - |
Homo sapiens | 3-hydroxyanthranilate + L-alanine | - |
? | |
3-hydroxy-L-kynurenine + H2O | L-tryptophan metabolism, biosynthesis of nicotinamide nucleotides | Homo sapiens | 3-hydroxyanthranilate + L-alanine | - |
? | |
L-kynurenine + H2O | - |
Homo sapiens | anthranilate + L-alanine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | native enzyme, 2 * 52500, disc gel electrophoresis | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
L-kynurenine hydrolase | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
- |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
3,7-dihydroxy-deamino-DL-kynurenine | pH 7.9, at 37ºC | Homo sapiens | |
0.012 | - |
D-kynurenine | pH 7.9, at 37ºC | Homo sapiens | |
0.02 | - |
L-kynurenine | pH 7.9, at 37ºC, at low substrate concentrations | Homo sapiens | |
0.055 | - |
L-kynurenine | pH 7.9, at 37ºC, at higher levels of substrate | Homo sapiens |