Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | neither EDTA, 2,2'-bipyridyl nor diethyldithiocarbamate have any effect upon enzyme activity. The activity is unaffected by reduced glutathione, 2-mercaptoethanol or L-cysteine (all at 1 mM) | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | Ag+ concentrations in the range 0.001-0.01 mM lower the activity to less than 50% of its uninhibited value | Pseudomonas putida | |
Cu2+ | Cu2+ concentrations in the range 0.001-0.01 mM lower the activity to less than 50% of its uninhibited value | Pseudomonas putida | |
iodoacetamide | 2 mM iodoacetate inhibits the enzyme activity to 50% or less of its uninhibited value after 15 min incubation with the enzyme at 0°C | Pseudomonas putida | |
p-chloromercuribenzoate | 0.2 mM p-chloromercuribenzoate inhibits the enzyme activity to 50% or less of its uninhibited value immediately after incubation with the enzyme at 0°C | Pseudomonas putida | |
sodium arsenite | 0.2 mM sodium arsenite inhibits the enzyme activity to 50% or less of its uninhibited value after 15 min incubation with the enzyme at 0°C | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.036 | - |
(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate | at pH 7.5 and 25°C | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O | Pseudomonas putida | - |
(2Z)-2-hydroxypenta-2,4-dienoate + acetate | - |
? | |
(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O | Pseudomonas putida ATCC 33015 | - |
(2Z)-2-hydroxypenta-2,4-dienoate + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
mt-2 | - |
Pseudomonas putida ATCC 33015 | - |
mt-2 | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, CM-cellulose column chromatography, DEAE-cellulose column chromatography, Sephadex G-100 gel filtration, and QAE-Sephadex A-50 gel filtration | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.58 | - |
cell-free extract, at pH 7.5 and 25°C | Pseudomonas putida |
48 | - |
after 82.8fold purification, at pH 7.5 and 25°C | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O | - |
Pseudomonas putida | (2Z)-2-hydroxypenta-2,4-dienoate + acetate | - |
? | |
(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O | 100% activity | Pseudomonas putida | (2Z)-2-hydroxypenta-2,4-dienoate + acetate | - |
? | |
(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O | - |
Pseudomonas putida ATCC 33015 | (2Z)-2-hydroxypenta-2,4-dienoate + acetate | - |
? | |
(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O | 100% activity | Pseudomonas putida ATCC 33015 | (2Z)-2-hydroxypenta-2,4-dienoate + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 31000-33000, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
2-hydroxymuconic semialdehyde hydrolase | - |
Pseudomonas putida |
HOD hydrolase | - |
Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | the enzyme is fairly stable to pH values between 5.5 to 9.0, with maximum stability at 7.0 to 8.0. Loss of activity is rapid at pH values below 5.0 and above 9.5 | Pseudomonas putida |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas putida | isoelectric focusing | - |
4.7 |