Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL-1 Blue cells | Paenarthrobacter nicotinovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
2,6-dihydroxypseudooxynicotine | in 75 mM phosphate buffer, pH 7.5, temperature not specified in the publication | Paenarthrobacter nicotinovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
gel filtration | Paenarthrobacter nicotinovorans |
43555 | - |
1 * 43555, calculated from amino acid sequence | Paenarthrobacter nicotinovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenarthrobacter nicotinovorans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-chelating Sepharose column chromatography | Paenarthrobacter nicotinovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-dihydroxypseudooxynicotine + H2O | - |
Paenarthrobacter nicotinovorans | 2,6-dihydroxypyridine + 4-methylaminobutanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 43000, SDS-PAGE | Paenarthrobacter nicotinovorans |
monomer | 1 * 43555, calculated from amino acid sequence | Paenarthrobacter nicotinovorans |
Synonyms | Comment | Organism |
---|---|---|
2,6-dihydroxypseudooxynicotine hydrolase | - |
Paenarthrobacter nicotinovorans |
DHPONH | - |
Paenarthrobacter nicotinovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
792 | - |
2,6-dihydroxypseudooxynicotine | in 75 mM phosphate buffer, pH 7.5, temperature not specified in the publication | Paenarthrobacter nicotinovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Paenarthrobacter nicotinovorans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | - |
Paenarthrobacter nicotinovorans |