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Literature summary for 3.7.1.19 extracted from

  • Sachelaru, P.; Schiltz, E.; Igloi, G.L.; Brandsch, R.
    An alpha/beta-fold C-C bond hydrolase is involved in a central step of nicotine catabolism by Arthrobacter nicotinovorans (2005), J. Bacteriol., 187, 8516-8519.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli XL-1 Blue cells Paenarthrobacter nicotinovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
2,6-dihydroxypseudooxynicotine in 75 mM phosphate buffer, pH 7.5, temperature not specified in the publication Paenarthrobacter nicotinovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
gel filtration Paenarthrobacter nicotinovorans
43555
-
1 * 43555, calculated from amino acid sequence Paenarthrobacter nicotinovorans

Organism

Organism UniProt Comment Textmining
Paenarthrobacter nicotinovorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-chelating Sepharose column chromatography Paenarthrobacter nicotinovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dihydroxypseudooxynicotine + H2O
-
Paenarthrobacter nicotinovorans 2,6-dihydroxypyridine + 4-methylaminobutanoate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 43000, SDS-PAGE Paenarthrobacter nicotinovorans
monomer 1 * 43555, calculated from amino acid sequence Paenarthrobacter nicotinovorans

Synonyms

Synonyms Comment Organism
2,6-dihydroxypseudooxynicotine hydrolase
-
Paenarthrobacter nicotinovorans
DHPONH
-
Paenarthrobacter nicotinovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
792
-
2,6-dihydroxypseudooxynicotine in 75 mM phosphate buffer, pH 7.5, temperature not specified in the publication Paenarthrobacter nicotinovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Paenarthrobacter nicotinovorans

pH Range

pH Minimum pH Maximum Comment Organism
5 10
-
Paenarthrobacter nicotinovorans