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Literature summary for 3.7.1.17 extracted from

  • Lack, N.; Kawamura, A.; Fullam, E.; Laurieri, N.; Beard, S.; Russell, A.; Evangelopoulos, D.; Westwood, I.; Sim, E.
    Temperature stability of proteins essential for the intracellular survival of Mycobacterium tuberculosis (2009), Biochem. J., 418, 369-378.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pseudomonas putida strain KT2442 Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid apparent value, in 100 mM of phosphate buffer (pH 7.4) with 20 mM NaCl, temperature not specified in the publication Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Mycobacterium tuberculosis 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33800
-
x * 33800, His6-tagged enzyme, estimated from SDS-PAGE Mycobacterium tuberculosis
132000
-
calculated from amino acid sequence Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
Mycobacterium tuberculosis ?
-
?
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
Mycobacterium tuberculosis H37Rv ?
-
?

Subunits

Subunits Comment Organism
homotetramer x * 33800, His6-tagged enzyme, estimated from SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase
-
Mycobacterium tuberculosis
HsaD
-
Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 70 the enzyme retains more than 95% of its activity after incubation at 60°C for 30 min. Hydrolytic activity is significantly decreased after incubation at 70°C Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.25
-
2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid in 100 mM of phosphate buffer (pH 7.4) with 20 mM NaCl, temperature not specified in the publication Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.129
-
2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid apparent value, in 100 mM of phosphate buffer (pH 7.4) with 20 mM NaCl, temperature not specified in the publication Mycobacterium tuberculosis