Cloned (Comment) | Organism |
---|---|
expressed in Pseudomonas putida strain KT2442 | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | apparent value, in 100 mM of phosphate buffer (pH 7.4) with 20 mM NaCl, temperature not specified in the publication | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mycobacterium tuberculosis | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33800 | - |
x * 33800, His6-tagged enzyme, estimated from SDS-PAGE | Mycobacterium tuberculosis |
132000 | - |
calculated from amino acid sequence | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O | - |
Mycobacterium tuberculosis | ? | - |
? | |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | x * 33800, His6-tagged enzyme, estimated from SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase | - |
Mycobacterium tuberculosis |
HsaD | - |
Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | the enzyme retains more than 95% of its activity after incubation at 60°C for 30 min. Hydrolytic activity is significantly decreased after incubation at 70°C | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | in 100 mM of phosphate buffer (pH 7.4) with 20 mM NaCl, temperature not specified in the publication | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.129 | - |
2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | apparent value, in 100 mM of phosphate buffer (pH 7.4) with 20 mM NaCl, temperature not specified in the publication | Mycobacterium tuberculosis |