BRENDA - Enzyme Database
show all sequences of 3.7.1.17

Temperature stability of proteins essential for the intracellular survival of Mycobacterium tuberculosis

Lack, N.; Kawamura, A.; Fullam, E.; Laurieri, N.; Beard, S.; Russell, A.; Evangelopoulos, D.; Westwood, I.; Sim, E.; Biochem. J. 418, 369-378 (2009)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Pseudomonas putida strain KT2442
Mycobacterium tuberculosis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.23
-
2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
apparent value, in 100 mM of phosphate buffer (pH 7.4) with 20 mM NaCl, temperature not specified in the publication
Mycobacterium tuberculosis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Mycobacterium tuberculosis
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33800
-
x * 33800, His6-tagged enzyme, estimated from SDS-PAGE
Mycobacterium tuberculosis
132000
-
calculated from amino acid sequence
Mycobacterium tuberculosis
Organism
Organism
UniProt
Commentary
Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Ni-NTA column chromatography, gel filtration
Mycobacterium tuberculosis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
717209
Mycobacterium tuberculosis
?
-
-
-
?
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
717209
Mycobacterium tuberculosis H37Rv
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotetramer
x * 33800, His6-tagged enzyme, estimated from SDS-PAGE
Mycobacterium tuberculosis
Synonyms
Synonyms
Commentary
Organism
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase
-
Mycobacterium tuberculosis
HsaD
-
Mycobacterium tuberculosis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
70
the enzyme retains more than 95% of its activity after incubation at 60°C for 30 min. Hydrolytic activity is significantly decreased after incubation at 70°C
Mycobacterium tuberculosis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.25
-
2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
in 100 mM of phosphate buffer (pH 7.4) with 20 mM NaCl, temperature not specified in the publication
Mycobacterium tuberculosis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Pseudomonas putida strain KT2442
Mycobacterium tuberculosis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.23
-
2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
apparent value, in 100 mM of phosphate buffer (pH 7.4) with 20 mM NaCl, temperature not specified in the publication
Mycobacterium tuberculosis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Mycobacterium tuberculosis
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33800
-
x * 33800, His6-tagged enzyme, estimated from SDS-PAGE
Mycobacterium tuberculosis
132000
-
calculated from amino acid sequence
Mycobacterium tuberculosis
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography, gel filtration
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
717209
Mycobacterium tuberculosis
?
-
-
-
?
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
717209
Mycobacterium tuberculosis H37Rv
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
x * 33800, His6-tagged enzyme, estimated from SDS-PAGE
Mycobacterium tuberculosis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
70
the enzyme retains more than 95% of its activity after incubation at 60°C for 30 min. Hydrolytic activity is significantly decreased after incubation at 70°C
Mycobacterium tuberculosis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.25
-
2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
in 100 mM of phosphate buffer (pH 7.4) with 20 mM NaCl, temperature not specified in the publication
Mycobacterium tuberculosis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.129
-
2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
apparent value, in 100 mM of phosphate buffer (pH 7.4) with 20 mM NaCl, temperature not specified in the publication
Mycobacterium tuberculosis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.129
-
2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
apparent value, in 100 mM of phosphate buffer (pH 7.4) with 20 mM NaCl, temperature not specified in the publication
Mycobacterium tuberculosis
Other publictions for EC 3.7.1.17
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733885
Ryan
Mechanism-based inhibition of ...
Mycobacterium tuberculosis
FEMS Microbiol. Lett.
350
42-47
2014
-
-
1
-
-
-
14
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
1
14
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
717815
Lack
Characterization of a carbon-c ...
Mycobacterium tuberculosis
J. Biol. Chem.
285
434-443
2010
-
-
1
1
1
-
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
2
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
4
-
-
-
-
-
-
-
-
3
3
717209
Lack
Temperature stability of prote ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Biochem. J.
418
369-378
2009
-
-
1
-
-
-
-
1
1
-
2
-
-
172
-
-
1
-
-
-
-
-
2
1
3
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
1
-
-
-
-
2
1
-
-
1
1
-
-
-
-
-
-
-
-
1
1
717030
Lack
Structure of HsaD, a steroid-d ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Acta Crystallogr. Sect. F
64
2-7
2007
-
-
1
1
-
-
-
-
-
-
-
2
-
172
-
-
1
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
718315
Van Der Geize
A gene cluster encoding choles ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Proc. Natl. Acad. Sci. USA
104
1947-1952
2007
-
-
1
-
-
-
-
1
-
-
-
-
-
172
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654270
Horinouchi
A new bacterial steroid degrad ...
Comamonas testosteroni, Comamonas testosteroni TA441
Appl. Environ. Microbiol.
69
4421-4430
2003
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-