BRENDA - Enzyme Database show
show all sequences of 3.7.1.17

Structure of HsaD, a steroid-degrading hydrolase, from Mycobacterium tuberculosis

Lack, N.; Lowe, E.; Liu, J.; Eltis, L.; Noble, M.; Sim, E.; Westwood, I.; Acta Crystallogr. Sect. F 64, 2-7 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Pseudomonas putida strain KT4224
Mycobacterium tuberculosis
Crystallization (Commentary)
Crystallization
Organism
sitting drop vapor diffusion method, using 30% (w/v) PEG 3000, 0.1?M CHES, pH 9.5
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid + H2O
Mycobacterium tuberculosis
high specificity
?
-
-
?
4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid + H2O
Mycobacterium tuberculosis H37Rv
high specificity
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-
Purification (Commentary)
Commentary
Organism
Ni affinity column chromatography
Mycobacterium tuberculosis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid + H2O
high specificity
717030
Mycobacterium tuberculosis
?
-
-
-
?
4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid + H2O
high specificity
717030
Mycobacterium tuberculosis H37Rv
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
x-ray crystallography
Mycobacterium tuberculosis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Pseudomonas putida strain KT4224
Mycobacterium tuberculosis
Crystallization (Commentary) (protein specific)
Crystallization
Organism
sitting drop vapor diffusion method, using 30% (w/v) PEG 3000, 0.1?M CHES, pH 9.5
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid + H2O
Mycobacterium tuberculosis
high specificity
?
-
-
?
4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid + H2O
Mycobacterium tuberculosis H37Rv
high specificity
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni affinity column chromatography
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid + H2O
high specificity
717030
Mycobacterium tuberculosis
?
-
-
-
?
4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid + H2O
high specificity
717030
Mycobacterium tuberculosis H37Rv
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
x-ray crystallography
Mycobacterium tuberculosis
Other publictions for EC 3.7.1.17
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733885
Ryan
Mechanism-based inhibition of ...
Mycobacterium tuberculosis
FEMS Microbiol. Lett.
350
42-47
2014
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1
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14
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1
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1
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1
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1
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1
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1
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1
14
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1
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1
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1
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-
717815
Lack
Characterization of a carbon-c ...
Mycobacterium tuberculosis
J. Biol. Chem.
285
434-443
2010
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1
1
1
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3
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1
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1
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3
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4
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1
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1
1
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3
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1
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3
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4
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3
3
717209
Lack
Temperature stability of prote ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Biochem. J.
418
369-378
2009
-
-
1
-
-
-
-
1
1
-
2
-
-
152
-
-
1
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-
-
-
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2
1
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-
1
1
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1
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1
1
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2
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1
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-
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2
1
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-
1
1
-
-
-
-
-
-
-
-
1
1
717030
Lack
Structure of HsaD, a steroid-d ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Acta Crystallogr. Sect. F
64
2-7
2007
-
-
1
1
-
-
-
-
-
-
-
2
-
152
-
-
1
-
-
-
-
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2
1
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1
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1
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2
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1
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2
1
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718315
Van Der Geize
A gene cluster encoding choles ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Proc. Natl. Acad. Sci. USA
104
1947-1952
2007
-
-
1
-
-
-
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1
-
-
-
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152
-
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-
-
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4
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1
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1
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4
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654270
Horinouchi
A new bacterial steroid degrad ...
Comamonas testosteroni, Comamonas testosteroni TA441
Appl. Environ. Microbiol.
69
4421-4430
2003
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4
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2
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2
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