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Literature summary for 3.7.1.13 extracted from

  • Habe, H.; Morii, K.; Fushinobu, S.; Nam, J.W.; Ayabe, Y.; Yoshida, T.; Wakagi, T.; Yamane, H.; Nojiri, H.; Omori, T.
    Crystal structure of a histidine-tagged serine hydrolase involved in the carbazole degradation (CarC enzyme) (2003), Biochem. Biophys. Res. Commun., 303, 631-639.
    View publication on PubMed
Search for more literature for 3.7.1.13

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas resinovorans
expression in Escherichia coli Janthinobacterium sp. J3

Crystallization (Commentary)

Crystallization (Comment) Organism
to 1.86 A resolution, space group I422. The subunit of ht-CarCJ3 is divided into two domains, i.e., the core domain with residues 15-144 and 209-282, and the lid domain with residues 145-208. The invisible 10 amino acid residues Ile146-Asn155 correspond to the helix alpha4, which is the putative first alpha-helix in the lid domain. Sidechains of the active-site residues, Ser114 and His261, are clearly shown in the electron density map Janthinobacterium sp. J3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00251
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate pH 7.5, 25°C Pseudomonas resinovorans
0.00273
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate pH 7.5, 25°C Janthinobacterium sp. J3

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 2 * 33000, SDS-PAGE Pseudomonas resinovorans
33000
-
 2 * 33000, SDS-PAGE Janthinobacterium sp. J3
70000
-
 gel filtration Pseudomonas resinovorans
70000
-
 gel filtration Janthinobacterium sp. J3

Organism

Organism UniProt Comment Textmining
Janthinobacterium sp. J3 Q84II3
-
-
Pseudomonas resinovorans
-
-
-
Pseudomonas resinovorans CA10
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Janthinobacterium sp. J3
recombinantv enzyme Pseudomonas resinovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 Pseudomonas resinovorans ?
-
 ?
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 Janthinobacterium sp. J3 ?
-
 ?
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 Pseudomonas resinovorans CA10 ?
-
 ?
2-hydroxy-6-oxohepta-2,4-dienoic acid + H2O 0.38% of the activity with 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate Pseudomonas resinovorans ?
-
 ?
2-hydroxy-6-oxohepta-2,4-dienoic acid + H2O 0.56% of the activity with 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate Janthinobacterium sp. J3 ?
-
 ?
2-hydroxy-6-oxohepta-2,4-dienoic acid + H2O 0.38% of the activity with 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate Pseudomonas resinovorans CA10 ?
-
 ?
2-hydroxymuconic semialdehyde + H2O 0.49% of the activity with 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate Janthinobacterium sp. J3 ?
-
 ?
2-hydroxymuconic semialdehyde + H2O 0.54% of the activity with 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate Pseudomonas resinovorans ?
-
 ?
2-hydroxymuconic semialdehyde + H2O 0.54% of the activity with 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate Pseudomonas resinovorans CA10 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 33000, SDS-PAGE Pseudomonas resinovorans
dimer 2 * 33000, SDS-PAGE Janthinobacterium sp. J3

Synonyms

Synonyms Comment Organism
CarC
-
 Pseudomonas resinovorans
CarC
-
 Janthinobacterium sp. J3

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.99
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate pH 7.5, 25°C Janthinobacterium sp. J3
2.14
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate pH 7.5, 25°C Pseudomonas resinovorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
730
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate pH 7.5, 25°C Janthinobacterium sp. J3
852
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate pH 7.5, 25°C Pseudomonas resinovorans