Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | inhibitory above 50 mM due to binding of the chloride anion in close neighborhood to the thiamine diphosphate cofactor | Azoarcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Azoarcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
2 * 59000, SDS-PAGE, 2 * 64500, calculated including FAD, thiamine diphosphate and Mg2+ | Azoarcus sp. |
64500 | - |
2 * 59000, SDS-PAGE, 2 * 64500, calculated including FAD, thiamine diphosphate and Mg2+ | Azoarcus sp. |
120000 | - |
gel filtration | Azoarcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azoarcus sp. | - |
- |
- |
Azoarcus sp. 22Lin | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cyclohexane-1,2-dione + H2O = 6-oxohexanoate | reaction starts with the monohydrated ketone of cyclohexane-1,2-dione and the thiamine diphosphate-ylide which undergoes a nucleophilic attack on the carbonyl group of cyclohexane-1,2-dione. The subsequent cleavage of the CĀC bond yields an alpha-carbanion, which is in equilibrium with its corresponding enamine. In the following step, the carbonic acid protonates the alpha-carbanion yielding 6-hydroxyhexanoate-thiamine diphosphate. Finally, the product 6-oxohexanoate is released | Azoarcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclohexane-1,2-dione + H2O | conversion of the monohydrated ketone form of cyclohexane-1,2-dione, rather than the monoenol form | Azoarcus sp. | 6-oxohexanoate | enzyme additionally catalyzes NAD+-dependent oxidation of 6-oxohexanoate to adipate | ? | |
cyclohexane-1,2-dione + H2O | conversion of the monohydrated ketone form of cyclohexane-1,2-dione, rather than the monoenol form | Azoarcus sp. 22Lin | 6-oxohexanoate | enzyme additionally catalyzes NAD+-dependent oxidation of 6-oxohexanoate to adipate | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 59000, SDS-PAGE, 2 * 64500, calculated including FAD, thiamine diphosphate and Mg2+ | Azoarcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Azoarcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Azoarcus sp. | |
thiamine diphosphate | - |
Azoarcus sp. |