Any feedback?
Literature summary for 3.7.1.1 extracted from
- Cloning and characterization of oah, the gene encoding oxaloacetate hydrolase in Aspergillus niger (2000), Mol. Gen. Genet., 263, 281-286.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned by inverse PCR into Escherichia coli | Aspergillus niger |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
Mn2+ | - |
Aspergillus niger |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
10-12 * 39000, SDS-PAGE | Aspergillus niger |
| 360000 | 440000 | two major bands, native PAGE | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oxaloacetate + H2O | Aspergillus niger | could be involved in pathogenesis and keeping pH low in vicinity of the fungus | oxalate + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
inducible enzyme, expressed only at pH values higher than 4.0 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Phenyl Sepharose, Q-Sepharose | Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 56 | - |
- |
Aspergillus niger |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 50 mM Tris-HCL, pH 7.5, 2 mM MnCl2, 20 mM DTT 5% sucrose, 7 days | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oxaloacetate + H2O | could be involved in pathogenesis and keeping pH low in vicinity of the fungus | Aspergillus niger | oxalate + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | 10-12 * 39000, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Aspergillus niger |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
