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Literature summary for 3.6.5.6 extracted from
- Inhibition of tubulin self-assembly and tubulin-colchicine GTPase activity by guanosine 5'-(gamma-fluorotriphosphate) (1987), Biochemistry, 26, 6091-6099.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Colchicine | induces GTPase activity | Bos taurus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| GTP | inhibitory effect produced at concentrations above 3 mM, above 5 mM leads to depolymerization of microtubules | Bos taurus | |
| guanosine 5'-(gamma-fluorotriphosphate) | reversible competitive dead-end inhibitor | Bos taurus | |
| guanosine 5'-(gamma-thiotriphosphate) | inhibits both tubulin polymerization and GTP hydrolysis | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.009 | - |
GTP | - |
Bos taurus | |
| 1.2 | 4.8 | GTP | - |
Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
- |
Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | Bos taurus | - |
GDP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | - |
Bos taurus | GDP + phosphate | - |
? | |
| additional information | ATP, UTP and CTP are no substrates | Bos taurus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tubulin-colchicine GTPase | - |
Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000233 | - |
GTP | - |
Bos taurus | |
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