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Literature summary for 3.6.5.5 extracted from
- Dynamin2 GTPase contributes to invadopodia formation in invasive bladder cancer cells (2016), Biochem. Biophys. Res. Commun., 480, 409-414 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene DNM2, enzyme expression analysis | Homo sapiens |
| gene DNM2, enzyme expression analysis, recombinant expression of GFP-tagged isozyme dynamin2 in human T24 cells | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | gene DNM2 knockout by RNAi in T24 cells | Homo sapiens |
| additional information | truncation of the proline/arginine-rich domain (PRD) of isozyme dynamin2 | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dynasore | dynamin specific inhibitor | Homo sapiens |  |
| dynasore | dynamin specific inhibitor | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| invadopodium | isozyme dynamin2 only | Rattus norvegicus | 71437 | - |
| invadopodium | isozyme dynamin2 only, localization in bladder cancer cells, overview | Homo sapiens | 71437 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Rattus norvegicus | |
| Mg2+ | required | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | Rattus norvegicus | - |
GDP + phosphate | - |
? | |
| GTP + H2O | Homo sapiens | - |
GDP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P50570 | - |
- |
| Rattus norvegicus | P39052 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | there are three dynamin isoforms in mammalian cells: dynamin1 (neuronal isoform), dynamin2 (ubiquitous isoform) and dynamin3 (a testis, lung and brain isoform) | Rattus norvegicus | - |
| additional information | there are three dynamin isoforms in mammalian cells: dynamin1 (neuronal isoform), dynamin2 (ubiquitous isoform) and dynamin3 (a testis, lung and brain isoform) | Homo sapiens | - |
| T-24 cell | - |
Homo sapiens | - |
| urinary bladder cancer cell | invasive bladder cancer cell | Rattus norvegicus | - |
| urinary bladder cancer cell | invasive bladder cancer cell | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | - |
Rattus norvegicus | GDP + phosphate | - |
? | |
| GTP + H2O | - |
Homo sapiens | GDP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | all dynamin isoforms share similar domain structures aligned from N-terminus to C-terminus: a GTPase domain, a middle domain, a PH domain, a GTPase effector domain (GED) and PRD domain | Homo sapiens |
| More | all dynamin isoforms share similar domain structures aligned from N-terminus to C-terminus: a GTPase domain, a middle domain, a PH domain, a GTPase effector domain (GED) and proline/arginine-rich domain (PRD) domain | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DNM2 | - |
Rattus norvegicus |
| DNM2 | - |
Homo sapiens |
| dynamin2 | - |
Rattus norvegicus |
| dynamin2 | - |
Homo sapiens |
| dynamin2 GTPase | - |
Rattus norvegicus |
| dynamin2 GTPase | - |
Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | there are three dynamin isoforms in mammalian cells: dynamin1 (neuronal isoform), dynamin2 (ubiquitous isoform) and dynamin3 (a testis, lung and brain isoform). All the dynamin isoforms share similar domain structures aligned from N-terminus to C-terminus: a GTPase domain, a middle domain, a PH domain, a GTPase effector domain (GED) and PRD domain | Rattus norvegicus |
| evolution | there are three dynamin isoforms in mammalian cells: dynamin1 (neuronal isoform), dynamin2 (ubiquitous isoform) and dynamin3 (a testis, lung and brain isoform). All the dynamin isoforms share similar domain structures aligned from N-terminus to C-terminus: a GTPase domain, a middle domain, a PH domain, a GTPase effector domain (GED) and PRD domain | Homo sapiens |
| malfunction | inhibition of dynamin2 function, using either RNA interference (RNAi) or the dynamin specific inhibitor Dynasore, causes defects in invadopodia formation and suppresses invasive activity of T24 bladder cancer cells | Rattus norvegicus |
| malfunction | inhibition of dynamin2 function, using either RNA interference (RNAi) or the dynamin specific inhibitor Dynasore, causes defects in invadopodia formation and suppresses invasive activity of T24 bladder cancer cells | Homo sapiens |
| additional information | structure-function analysis using dynamin2 deletion fragments identifies the proline/arginine-rich domain (PRD) of dynamin2 as indispensable for invadopodia formation and invasiveness of cancer cells | Rattus norvegicus |
| additional information | structure-function analysis using dynamin2 deletion fragments identifies the proline/arginine-rich domain (PRD) of dynamin2 as indispensable for invadopodia formation and invasiveness of T24 cells | Homo sapiens |
| physiological function | dynamin is a large GTPase essential for membrane fission in clathrin-mediated endocytosis. There are three dynamin isoforms in mammalian cells. Isozyme dynamin2 GTPase contributes to invadopodia formation in invasive bladder cancer cells. Cancer cell invasion is mediated by actin-based membrane protrusions termed invadopodia. Invadopodia consist of core F-actin bundles associated with adhesive and proteolytic machineries promoting cell invasion by degrading extracellular matrix (ECM). Formation of the F-actin core in invadopodia is regulated by various actin-binding proteins including Arp2/3 complex and cortactin. Dynamin GTPase localizes to the invadopodia and is implicated in cancer cell invasion. Dynamin2 contributes to bladder cancer invasion by controlling invadopodia formation in bladder cancer cells | Rattus norvegicus |
| physiological function | dynamin is a large GTPase essential for membrane fission in clathrin-mediated endocytosis. There are three dynamin isoforms in mammalian cells. Isozyme dynamin2 GTPase contributes to invadopodia formation in invasive bladder cancer cells. Cancer cell invasion is mediated by actin-based membrane protrusions termed invadopodia. Invadopodia consist of core F-actin bundles associated with adhesive and proteolytic machineries promoting cell invasion by degrading extracellular matrix (ECM). Formation of the F-actin core in invadopodia is regulated by various actin-binding proteins including Arp2/3 complex and cortactin. Dynamin GTPase localizes to the invadopodia and is implicated in cancer cell invasion. Dynamin2 contributes to bladder cancer invasion by controlling invadopodia formation in bladder cancer cells | Homo sapiens |
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