Cloned (Comment) | Organism |
---|---|
recombinant expression of GST- and His-tagged MxA in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D250N | site-directed mutagenesis, a monomeric mutant, loss of nucleotide binding | Homo sapiens |
D253N | site-directed mutagenesis, a monomeric mutant | Homo sapiens |
G255E | site-directed mutagenesis, a monomeric mutant | Homo sapiens |
K83A | site-directed mutagenesis, the mutant shows reduced dimerization | Homo sapiens |
M527D | site-directed mutagenesis, a monomeric mutant | Homo sapiens |
M527D/D250N | site-directed mutagenesis, a monomeric mutant | Homo sapiens |
M527D/D250N/D253N | site-directed mutagenesis, a monomeric mutant | Homo sapiens |
M527D/D253N | site-directed mutagenesis, a monomeric mutant | Homo sapiens |
M527D/K83A | site-directed mutagenesis, a monomeric mutant | Homo sapiens |
M527D/T103A | site-directed mutagenesis, a monomeric mutant | Homo sapiens |
additional information | analysis of nucleotide binding, dimerization, and GTPase activities of the enzyme mutants, overview | Homo sapiens |
T103A | site-directed mutagenesis, a monomeric mutant | Homo sapiens |
V268M | site-directed mutagenesis, the mutant shows reduced dimerization | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | the enzyme is expressed in the cytoplasm and is partly associated with membranes of the smooth endoplasmic reticulum | Homo sapiens | 5737 | - |
endoplasmic reticulum | the enzyme is expressed in the cytoplasm and is partly associated with membranes of the smooth endoplasmic reticulum | Homo sapiens | 5783 | - |
membrane | the enzyme is expressed in the cytoplasm and is partly associated with membranes of the smooth endoplasmic reticulum | Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Homo sapiens | - |
GDP + phosphate | - |
? | |
additional information | Homo sapiens | MxA recognizes Thogoto virus nucleocapsid proteins independently of nucleotide binding, while both nucleotide binding and hydrolysis are required for the antiviral activity against Thogoto, influenza, and La Crosse viruses. GTP binding facilitates formation of stable MxA assemblies associated with endoplasmic reticulum membranes, whereas nucleotide hydrolysis promotes dynamic redistribution of MxAfrom cellular membranes to viral targets | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P20591 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST- and His-tagged MxA from Escherichia coli strain BL21(DE3) by nickel affinity and glutathione affinity chromatography, and gel filtration | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
GTP + H2O = GDP + phosphate | analysis of the GTPase mechanism of MxA | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Homo sapiens | GDP + phosphate | - |
? | |
additional information | MxA recognizes Thogoto virus nucleocapsid proteins independently of nucleotide binding, while both nucleotide binding and hydrolysis are required for the antiviral activity against Thogoto, influenza, and La Crosse viruses. GTP binding facilitates formation of stable MxA assemblies associated with endoplasmic reticulum membranes, whereas nucleotide hydrolysis promotes dynamic redistribution of MxAfrom cellular membranes to viral targets | Homo sapiens | ? | - |
? | |
additional information | enzyme-nucleotide binding studies, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | dimerization of MxA via a GTPase domain interface is required for GTP hydrolysis and antiviral activity. Residues in the catalytic center of MxA and the nucleotide itself are essential for G domain dimerization and catalytic activation | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
dynamin-like myxovirus resistance protein A | - |
Homo sapiens |
Mx GTPase | - |
Homo sapiens |
MX1 | - |
Homo sapiens |
MxA | - |
Homo sapiens |
myxovirus resistance GTPase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | homology modeling and comparison to human dynamin1 GTPase structure, overview. Most of the residues in the catalytic center are highly conserved between MxA and dynamin, for example the G4 loop mediating specificity for guanine binding | Homo sapiens |
physiological function | myxovirus resistance proteins are key mediators of the interferon-induced innate immune response in vertebrates, role of nucleotide binding and GTPase domain dimerization in dynamin-like myxovirus resistance protein A for GTPase activation and antiviral activity. GTP binding allows GTPase domain dimerization and membrane-associated assembly of MxA, but it is not sufficient to induce a sustained antiviral effect | Homo sapiens |