Cloned (Comment) | Organism |
---|---|
a 6-His N-terminal-tagged human dynamin2, lacking the C-terminal proline-rich domain, is constructed, cloned into the pET28a vector for expression in Escherichia coli BL21DE3 cells | Homo sapiens |
human dynamin is expressed in SF9 insect cells, using the Bac-to-Bac baculovirus expression system a full-length cDNA encoding human dynamin1 containing a 6His-tag is subcloned into pFastBac, a bacmid is generated after transposition in Escherichia coli | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dynasore | noncompetitive inhibitor of dynamin GTPase activity | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
- |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Homo sapiens | - |
GDP + phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA and hydroxyapatite chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Homo sapiens | GDP + phosphate | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
dynamin | - |
Homo sapiens |
dynamin1 | - |
Homo sapiens |
dynamin2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
colorimetric GTPase activity assay at room temperature | Homo sapiens |
22 | - |
radioactive assay for the GTPase activity | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
radioactive assay for the GTPase activity | Homo sapiens |
7.5 | - |
colorimetric GTPase activity assay | Homo sapiens |