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Literature summary for 3.6.5.5 extracted from

  • Kirchhausen, T.; Macia, E.; Pelish, H.E.
    Use of dynasore, the small molecule inhibitor of dynamin, in the regulation of endocytosis (2008), Methods Enzymol., 438, 77-93.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
a 6-His N-terminal-tagged human dynamin2, lacking the C-terminal proline-rich domain, is constructed, cloned into the pET28a vector for expression in Escherichia coli BL21DE3 cells Homo sapiens
human dynamin is expressed in SF9 insect cells, using the Bac-to-Bac baculovirus expression system a full-length cDNA encoding human dynamin1 containing a 6His-tag is subcloned into pFastBac, a bacmid is generated after transposition in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
dynasore noncompetitive inhibitor of dynamin GTPase activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Homo sapiens
-
GDP + phosphate
-
ir

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA and hydroxyapatite chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
Homo sapiens GDP + phosphate
-
ir

Synonyms

Synonyms Comment Organism
dynamin
-
Homo sapiens
dynamin1
-
Homo sapiens
dynamin2
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
colorimetric GTPase activity assay at room temperature Homo sapiens
22
-
radioactive assay for the GTPase activity Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
radioactive assay for the GTPase activity Homo sapiens
7.5
-
colorimetric GTPase activity assay Homo sapiens