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Literature summary for 3.6.5.5 extracted from
- Mitotic phosphorylation of dynamin-related GTPase Drp1 participates in mitochondrial fission (2007), J. Biol. Chem., 282, 11521-11529.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the cDNA of rat Drp1 is subcloned into pEGFP-N1 and p3xFLAG-CMV-10 for transfection of HeLa cells, and in pET28a for expression in Escherichia coli | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S126A | mutation in the potential recognition site for Cdk1/cyclin B | Rattus norvegicus |
| S136A | mutation in the potential recognition site for Cdk1/cyclin B | Rattus norvegicus |
| S585A | exogenous expression of unphosphorylated mutant S585A leads to reduced mitotic mitochondrial fragmentation | Rattus norvegicus |
| S71A | mutation in the potential recognition site for Cdk1/cyclin B | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | human Drp1 is knocked down in HeLa cells by the specific siRNA | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | Rattus norvegicus | - |
GDP + phosphate | - |
ir |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | Drp1 is specifically phosphorylated in mitosis by Cdk1/cyclin B on Ser-585 | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-Drp1 proteins are purified by metal-chelating resin and further purified by ion exchange chromatography | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | - |
Rattus norvegicus | GDP + phosphate | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Drp1 | - |
Rattus norvegicus |
| dynamin-retated GTPase Drp1 | - |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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