Cloned (Comment) | Organism |
---|---|
the cDNA of rat Drp1 is subcloned into pEGFP-N1 and p3xFLAG-CMV-10 for transfection of HeLa cells, and in pET28a for expression in Escherichia coli | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
S126A | mutation in the potential recognition site for Cdk1/cyclin B | Rattus norvegicus |
S136A | mutation in the potential recognition site for Cdk1/cyclin B | Rattus norvegicus |
S585A | exogenous expression of unphosphorylated mutant S585A leads to reduced mitotic mitochondrial fragmentation | Rattus norvegicus |
S71A | mutation in the potential recognition site for Cdk1/cyclin B | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | human Drp1 is knocked down in HeLa cells by the specific siRNA | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Rattus norvegicus | - |
GDP + phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | Drp1 is specifically phosphorylated in mitosis by Cdk1/cyclin B on Ser-585 | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
recombinant His-Drp1 proteins are purified by metal-chelating resin and further purified by ion exchange chromatography | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Rattus norvegicus | GDP + phosphate | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
Drp1 | - |
Rattus norvegicus |
dynamin-retated GTPase Drp1 | - |
Rattus norvegicus |