Literature summary for 3.6.5.5 extracted from

Lee, C.S.; Kim, I.S.; Park, J.B.; Lee, M.N.; Lee, H.Y.; Suh, P.G.; Ryu, S.H.
The phox homology domain of phospholipase D activates dynamin GTPase activity and accelerates EGFR endocytosis (2006), Nat. Cell Biol., 8, 477-484.

Search for more literature for 3.6.5.5

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	the GAP function of PLD1-PX stimulates the GTPase activity of dynamin by increasing dynamin self-assembly	Rattus norvegicus
phospholipase D	PLD function as a GTPase activating protein, GAP, through its phox homology domain, PX, which directly activates the GTPase domain of dynamin	Rattus norvegicus

Cloned(Commentary)

Cloned (Comment)	Organism
into the pFLAG-CMV-2 vector, five functional dynamin domains, GTPase, Middle, pleckstrin homology, GTPase effector and proline rich, are subcloned into pGEX-4T2	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
clathrin-coated vesicle	clathrin-coated pit	Rattus norvegicus	30136	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GTP + H2O	Rattus norvegicus	-	GDP + phosphate	-	ir

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
glutathione S-transferase-fused proteins are purified from Escherichia coli strain BL21 using glutathione-Sepharose, full-length dynamin is purified from rat brain	Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.16	-	the specific rate of GTP hydrolysis of dynamin stimulated by the PLDs-PX	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + H2O	-	Rattus norvegicus	GDP + phosphate	-	ir

Subunits

Subunits	Comment	Organism
More	dynamin is a multidomain protein that self-assembles into higher order structures resembling rings during endocytosis	Rattus norvegicus

Synonyms

Synonyms	Comment	Organism
dynamin	-	Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	GTPase assay at room temperature	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	GTPase assay	Rattus norvegicus

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Release 2023.1 (January 2023)