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Literature summary for 3.6.5.5 extracted from

  • Reubold, T.F.; Eschenburg, S.; Becker, A.; Leonard, M.; Schmid, S.L.; Vallee, R.B.; Kull, F.J.; Manstein, D.J.
    Crystal structure of the GTPase domain of rat dynamin 1 (2005), Proc. Natl. Acad. Sci. USA, 102, 13093-13098.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
lipid tubule stimulate GTPase activity Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop method, 1.9 A crystal structure of the nucleotide-free GTPase domain of dynamin 1 Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
R59A kcat is 1.5fold lower than wild-type value, KM is 5.2fold higher than wild-type value Rattus norvegicus
R59K kcat is 3fold lower than wild-type value, KM is 2fold higher than wild-type value Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.046
-
GTP 37°C, wild-type enzyme Rattus norvegicus
0.096
-
GTP 37°C, mutant enzyme R59K Rattus norvegicus
0.241
-
GTP 37°C, mutant enzyme R59A Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Rattus norvegicus
-
GDP + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
Rattus norvegicus GDP + phosphate
-
?

Synonyms

Synonyms Comment Organism
dynamin 1
-
Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0079
-
GTP 37°C, mutant enzyme R59K Rattus norvegicus
0.016
-
GTP 37°C, mutant enzyme R59A Rattus norvegicus
0.024
-
GTP 37°C, wild-type enzyme Rattus norvegicus