Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | dynamin can self-assemble forming higher order structures such as rings and spirals that exhibit up to 100fold stimulated GTPase activity, conformational changes in the active site accompany self-assembly | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene for dynamin1, expression in HEK293-cre4 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | effect of dynamin GTPase domain mutations on GTP binding, GTP hydrolysis and clathrin-mediated endocytosis | Homo sapiens |
S61A | mutation lowers the rate of assembly-stimulated GTP hydrolysis without altering the Km for GTP, kinetic data | Homo sapiens |
S61D | kinetic data | Homo sapiens |
T141A | mutation increases the rate of assembly-stimulated GTP hydrolysis without altering the Km for GTP, kinetic data | Homo sapiens |
T141D | kinetic data | Homo sapiens |
T65A | mutation dramatically lowers both the rate of assembly-stimulated GTP hydrolysis and the affinity for GTP, kinetic data | Homo sapiens |
T65D | mutation dramatically lowers both the rate of assembly-stimulated GTP hydrolysis and the affinity for GTP, kinetic data | Homo sapiens |
T65H | mutation dramatically lowers both the rate of assembly-stimulated GTP hydrolysis and the affinity for GTP, kinetic data | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data, temperature-dependent effects on kinetic parameters, self-assembly alters the Km for GTP | Homo sapiens | |
0.0034 | - |
GTP | 22°C, wild-type dynamin, basal GTPase activity | Homo sapiens | |
0.0053 | - |
GTP | 22°C, wild-type dynamin, lipid tubule stimulated GTPase activity | Homo sapiens | |
0.037 | - |
GTP | 37°C, wild-type dynamin, lipid tubule stimulated GTPase activity | Homo sapiens | |
0.041 | - |
GTP | 37°C, S61A mutant dynamin: lipid tubule stimulated GTPase activity, T65D mutant dynamin: basal GTPase activity | Homo sapiens | |
0.056 | - |
GTP | 37°C, S61D and T141A mutant dynamin, lipid tubule stimulated GTPase activity | Homo sapiens | |
0.067 | - |
GTP | 37°C, S61A mutant dynamin, basal GTPase activity | Homo sapiens | |
0.068 | - |
GTP | 37°C, S61D mutant dynamin, basal GTPase activity | Homo sapiens | |
0.102 | - |
GTP | 37°C, wild-type dynamin, basal GTPase activity | Homo sapiens | |
0.149 | - |
GTP | 37°C, T65H mutant dynamin, basal GTPase activity | Homo sapiens | |
0.174 | - |
GTP | 37°C, T141A mutant dynamin, basal GTPase activity | Homo sapiens | |
0.513 | - |
GTP | 37°C, T65D mutant dynamin, lipid tubule stimulated GTPase activity | Homo sapiens | |
0.763 | - |
GTP | 37°C, T141D mutant dynamin, basal GTPase activity | Homo sapiens | |
0.88 | - |
GTP | 37°C, T65A mutant dynamin, basal GTPase activity | Homo sapiens | |
0.988 | - |
GTP | 37°C, T65H mutant dynamin, lipid tubule stimulated GTPase activity | Homo sapiens | |
1.94 | - |
GTP | 37°C, T141D mutant dynamin, lipid tubule stimulated GTPase activity | Homo sapiens | |
2.115 | - |
GTP | 37°C, T65A mutant dynamin, lipid tubule stimulated GTPase activity | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Homo sapiens | GTP hydrolysis is required for clathrin-mediated endocytosis in vivo | GDP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
recombinant dynamin1 | - |
Purification (Comment) | Organism |
---|---|
recombinant dynamin-1 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | GTP hydrolysis is required for clathrin-mediated endocytosis in vivo | Homo sapiens | GDP + phosphate | - |
? | |
GTP + H2O | conformational changes in the active site accompany self-assembly, dynamin domain structure, Thr-65 plays an important role in catalysis, Thr-141 has roles in both GTP binding and hydrolysis | Homo sapiens | GDP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | dynamin can self-assemble forming higher order structures such as rings and spirals that exhibit up to 100fold stimulated GTPase activity | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
dynamin 1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 37 | both the basal and assembly-stimulated rates of GTP hydrolysis for wild-type dynamin are 10fold lower when assayed at 22°C compared with 37°C, effect of these temperatures on the Km | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data, temperature-dependent effects on kinetic parameters | Homo sapiens | |
0.0003 | - |
GTP | 37°C, T65D and T65H mutant dynamin, basal GTPase activity | Homo sapiens | |
0.0013 | - |
GTP | 37°C, T65A mutant dynamin, basal GTPase activity | Homo sapiens | |
0.0025 | - |
GTP | 37°C, T141D mutant dynamin, basal GTPase activity | Homo sapiens | |
0.0032 | - |
GTP | 22°C, wild-type dynamin, basal GTPase activity | Homo sapiens | |
0.0072 | - |
GTP | 37°C, S61D mutant dynamin, basal GTPase activity | Homo sapiens | |
0.016 | - |
GTP | 37°C, T65D and T65H mutant dynamin, lipid tubule stimulated GTPase activity | Homo sapiens | |
0.02 | - |
GTP | 37°C, T65A mutant dynamin, lipid tubule stimulated GTPase activity | Homo sapiens | |
0.025 | - |
GTP | 37°C, T141D mutant dynamin, lipid tubule stimulated GTPase activity | Homo sapiens | |
0.032 | - |
GTP | 37°C, S61A mutant dynamin, basal GTPase activity | Homo sapiens | |
0.043 | - |
GTP | 37°C, wild-type dynamin, basal GTPase activity | Homo sapiens | |
0.112 | - |
GTP | 37°C, T141A mutant dynamin, basal GTPase activity | Homo sapiens | |
0.13 | - |
GTP | 22°C, wild-type dynamin, lipid tubule stimulated GTPase activity | Homo sapiens | |
0.44 | - |
GTP | 37°C, S61D mutant dynamin, lipid tubule stimulated GTPase activity | Homo sapiens | |
1.43 | - |
GTP | 37°C, S61A mutant dynamin, lipid tubule stimulated GTPase activity | Homo sapiens | |
1.75 | - |
GTP | 37°C, wild-type dynamin, lipid tubule stimulated GTPase activity | Homo sapiens | |
3.08 | - |
GTP | 37°C, T141A mutant dynamin, lipid tubule stimulated GTPase activity | Homo sapiens |