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Literature summary for 3.6.5.4 extracted from

  • Wild, K.; Juaire, K.D.; Soni, K.; Shanmuganathan, V.; Hendricks, A.; Segnitz, B.; Beckmann, R.; Sinning, I.
    Reconstitution of the human SRP system and quantitative and systematic analysis of its ribosome interactions (2019), Nucleic Acids Res., 47, 3184-3196 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.5.4

Cloned(Commentary)

Cloned (Comment) Organism
recombinant large-scale production of all human SRP components and the reconstitution of homogeneous signal recognition particle (SRP) and signal recognition particle receptor (SR) complexes Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information two-site binding analysis of the SRP54DELTAC-ribosome interaction. Having a signal sequence fused to SRP54 or presented by the ribosome does not seem to significantly change SRP binding to the ribosome Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane
-
 Homo sapiens 5789
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Homo sapiens
-
 GDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P08240 AND Q9Y5M8 subunits SRalpha and SRbeta
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information separating SRP biogenesis in two parts, a nucleolar and a cytosolic one, is essential for correct SRP assembly Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Homo sapiens GDP + phosphate
-
 ?
additional information both SRalpha and SRbeta are GTPases Homo sapiens ?
-
-

Subunits

Subunits Comment Organism
heterodimer
-
 Homo sapiens

Synonyms

Synonyms Comment Organism
signal recognition particle receptor subunit alpha
-
 Homo sapiens
signal recognition particle receptor subunit beta
-
 Homo sapiens
SRalpha
-
 Homo sapiens
SRbeta
-
 Homo sapiens
SRP GTPase
-
 Homo sapiens
SRPRA gene name, subunit SRalpha Homo sapiens
SRPRB gene name, subunit SRbeta Homo sapiens

General Information

General Information Comment Organism
additional information reconstitution of the human SRP system and quantitative and systematic analysis of its ribosome interactions, as well as interactions within the SRP complex, overview. SRP RNA does not bind to the ribosome, while signal recognition particle (SRP) binds with nanomolar affinity involving a two-step mechanism of the key-player SRP GTPase, SRP54. SRP54 is the major determinant of SRP-ribosome interaction. Modulation of SRP-ribosome interaction by signal recognition particle receptor (SR). As long as SRP54 is not assembled into SRP, ribosome binding is of moderate affinity at most. Separating SRP biogenesis in two parts, a nucleolar and a cytosolic one, is essential for correct SRP assembly. Affinity of the entire recombinant SRPS/SR system to RNCs exposing an SRP-targeting signal Homo sapiens
physiological function co-translational protein targeting to membranes depends on the regulated interaction of two ribonucleoprotein particles (RNPs): the ribosome and the signal recognition particle (SRP). Human SRP is composed of an SRP RNA and six proteins with the SRP GTPase SRP54 forming the targeting complex with the heterodimeric SRP receptor (SRalphabeta) at the endoplasmic reticulum membrane Homo sapiens