Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cpFtsY | chlorplastic cell division protein FtsY homologue, a signal recognition particle receptor protein, also shows GTPase activity | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
gene CPSRP54, recombinant overexpression of His6-tagged signal recognition particle protein cpSRP54 in Escherichia coli strain Rosetta, coexpression with signal recognition particle activator protein cpFtsY forming the cpSRP54-cpFtsY complex | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic measurements are performed using a KinTek stopped-flow apparatus with active GTP-bound cpSRP54-cpFtsY complex | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Arabidopsis thaliana | - |
GDP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P37107 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged signal recognition particle protein cpSRP54 from Escherichia coli strain Rosetta by nickel affinity chromatography, cation exchange chromatography, and anion exchange chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Arabidopsis thaliana | GDP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cpSRP54 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | co-evolution of two GTPases enables efficient protein targeting in an RNA-less chloroplast signal recognition particle pathway | Arabidopsis thaliana |
additional information | the SRP RNAis a universally conserved component of SRP that mediates key interactions between two GTPases in SRP and its receptor, thus enabling rapid delivery of cargo to the target membrane. Notably, this essential RNA is bypassed in the chloroplast (cp) SRP of green plants. The cpSRP and cpSRP receptor GTPases (cpSRP54 and cpFtsY, respectively) interact efficiently by themselves without the SRP RNA, molecular mechanism, overview | Arabidopsis thaliana |
physiological function | the signal recognition particle (SRP) is an essential ribonucleoprotein particle that mediates the co-translational targeting of newly synthesized proteins to cellular membranes | Arabidopsis thaliana |