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Literature summary for 3.6.5.4 extracted from

  • Siu, F.Y.; Spanggord, R.J.; Doudna, J.A.
    SRP RNA provides the physiologically essential GTPase activation function in cotranslational protein targeting (2007), RNA, 13, 240-250.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
4.5S RNA stimulates GTPase activity in the SRP-FtsY complex in vitro and is essential for SRP function in vivo Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information 4.5S RNA tetraloop mutants UUCG, UUUU, CUUC, GUAA and GAAA Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol the SRP targets the ribosome-nascent chain complexes to the inner membrane by interacting with the SRP receptor Escherichia coli 5829
-
inner membrane the SRP targets the ribosome-nascent chain complexes to the inner membrane by interacting with the SRP receptor Escherichia coli
-
-
ribosome the SRP targets the ribosome-nascent chain complexes to the inner membrane by interacting with the SRP receptor Escherichia coli 5840
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Escherichia coli
-
GDP + phosphate
-
ir

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
ribonucleoprotein SRP comprises the Ffh protein and 4.5S RNA, which together recognize hydrophobic signal peptides as they emerge from translating ribosomes Escherichia coli

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
Escherichia coli GDP + phosphate
-
ir

Subunits

Subunits Comment Organism
heterodimer Ffh and FtsY associate into a heterodimer upon GTP binding, upon GTP hydrolysis Ffh and FtsY dissociate Escherichia coli

Synonyms

Synonyms Comment Organism
signal recognition particle
-
Escherichia coli
SRP
-
Escherichia coli
SRP GTPase
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.021
-
GTP maximal rate constant for the GTPase activity of the SRP-FtsY complex, UUUU mutant Escherichia coli
0.037
-
GTP maximal rate constant for the GTPase activity of the SRP-FtsY complex, no RNA Escherichia coli
0.053
-
GTP maximal rate constant for the GTPase activity of the SRP-FtsY complex, UUCG mutant Escherichia coli
0.14
-
GTP maximal rate constant for the GTPase activity of the SRP-FtsY complex, GUAA mutant Escherichia coli
0.182
-
GTP maximal rate constant for the GTPase activity of the SRP-FtsY complex, GAAA mutant Escherichia coli
0.223
-
GTP maximal rate constant for the GTPase activity of the SRP-FtsY complex, wild-type RNA Escherichia coli
0.253
-
GTP maximal rate constant for the GTPase activity of the SRP-FtsY complex, CUUC mutant Escherichia coli